DLGAP1 (NM_001242763) Human Tagged ORF Clone

CAT#: RG234738

DLGAP1 (tGFP-tagged) - Homo sapiens discs, large (Drosophila) homolog-associated protein 1 (DLGAP1), transcript variant 5

Product Images

Circular map for RG234738

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DLGAP1
• Synonyms: DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1A; DLGAP1B; GKAP; SAPAP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234738 representing NM_001242763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTGAAGACATTAAAACTGTTTAATTCTCAGTTGAGATGTGGTTGGCTCTTATACATTTGGAATA
  AAGCATTTATGGCACATCTCAGGGTTCCACAAGATGAATGGACAGGGTACACCCCACGAGGTAAAGATGA
  TGAAATTCCATGCCGAAGAATGCGGAGTGGCAGTTATATCAAGGCCATGGGGGATGAAGACAGTGGAGAC
  TCAGACACGAGTCCTAAGCCTTCTCCAAAAGTTGCTGCGCGGAGAGAAAGCTATCTCAAGGCTACTCAGC
  CATCCCTTACAGAACTCACCACACTCAAAATCTCCAATGAACACTCACCCAAACTCCAGATCCGGAGTCA
  TAGTTACCTGAGGGCAGTGAGTGAAGTCTCCATCAACCGGAGCCTGGACAGCCTGGACCCTGCAGGCTTG
  CTCACATCACCAAAGTTCCGCTCCAGGAATGAGAGCTACATGCGAGCCATGAGCACCATCAGCCAGGTGA
  GCGAGATGGAAGTGAACGGGCAGTTCGAGTCCGTGTGCGAGTCCGTGTTCAGCGAGCTGGAGTCGCAGGC
  CGTGGAAGCGCTGGACCTGCCCATGCCCGGCTGCTTCCGCATGCGGAGCCACAGCTATGTGCGGGCCATT
  GAGAAAGGCTGCTCCCAGGACGACGAGTGCGTGTCCCTGAGGTCGTCCTCGCCGCCGCGCACCACCACCA
  CCGTTAGGACCATCCAGAGCAGCACGGGTGTCATAAAACTGAGTTCTGCAGTTGAAGTGTCATCTTGCAT
  TACAACATATAAGAAGACACCACCTCCAGTCCCACCCAGAACTACCACGAAACCTTTCATTTCTATCACA
  GCCCAGAGTAGCACAGAGTCAGCCCAGGATGCCTACATGGACGGACAGGGCCAGCGAGGAGATATTATCA
  GCCAGTCTGGACTCAGCAACTCCACCGAGAGCCTGGACAGTATGAAGGCTCTGACAGCCGCCATCGAAGC
  TGCAAACGCCCAGATCCATGGCCCTGCCAGTCAACACATGGGCAATAACACTGCCACCGTCACCACCACG
  ACTACCATAGCCACCGTCACCACGGAGGACAGGAAGAAGGACCACTTTAAGAAAAATCGATGCCTGTCTA
  TCGGGATACAGGTGGATGATGCTGAAGAACCTGACAAAACAGGGGAGAATAAAGCACCCAGTAAGTTCCA
  GTCCGTGGGAGTGCAAGTAGAAGAAGAGAAGTGCTTCCGCAGGTTCACTCGATCCAACAGTGTGACGACA
  GCAGTACAGGCCGACCTGGACTTCCATGATAATCTGGAAAATTCTCTGGAATCTATAGAGGACAATTCGT
  GTCCTGGCCCCATGGCCAGACAGTTCTCCCGCGATGCCAGCACCTCCACAGTCAGCATTCAGGGCTCAGG
  AAACCATTACCATGCCTGTGCCGCCGATGATGACTTTGACACGGATTTTGACCCCTCTATTCTGCCTCCT
  CCGGACCCCTGGATTGACTCTATCACTGAAGACCCTCTGGAGGCCGTGCAAAGGTCAGTGTGCCACCGGG
  ATGGCCACTGGTTCCTGAAGCTTCTCCAGGCAGAGCGAGACCGCATGGAGGGGTGGTGTCAACAGATGGA
  GCGGGAAGAACGGGAAAACAACCTGCCCGAAGACATTCTAGGAAAAATCCGAACCGCAGTGGGCAGTGCC
  CAACTTCTCATGGCCCAGAAATTCTACCAGTTCAGAGAACTGTGTGAAGAAAACCTGAATCCTAATGCTC
  ATCCAAGACCCACCTCCCAGGATTTGGCGGGGTTTTGGGACATGCTGCAGTTGTCCATAGAAAATATTAG
  TATGAAATTTGATGAACTTCATCAGTTAAAGGCCAATAATTGGAAACAGATGGATCCTCTTGACAAGAAG
  GAGAGAAGGGCCCCTCCTCCAGTGCCAAAGAAGCCGGCGAAGGGCCCCGCGCCGCTGATCCGGGAGCGCT
  CGCTGGAGAGCTCGCAGCGCCAGGAGGCCCGCAAGCGCCTGATGGCCGCCAAGCGCGCCGCGTCCGTCCG
  CCAGAACTCGGCCACCGAGAGCGCCGAGAGCATCGAGATCTACATCCCCGAGGCGCAGACCCGGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234738 representing NM_001242763
  Red=Cloning site Green=Tags(s)
  
  MDLKTLKLFNSQLRCGWLLYIWNKAFMAHLRVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKAMGDEDSGD
  SDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSLDSLDPAGL
  LTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMRSHSYVRAI
  EKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTGVIKLSSAVEVSSCITTYKKTPPPVPPRTTTKPFISIT
  AQSSTESAQDAYMDGQGQRGDIISQSGLSNSTESLDSMKALTAAIEAANAQIHGPASQHMGNNTATVTTT
  TTIATVTTEDRKKDHFKKNRCLSIGIQVDDAEEPDKTGENKAPSKFQSVGVQVEEEKCFRRFTRSNSVTT
  AVQADLDFHDNLENSLESIEDNSCPGPMARQFSRDASTSTVSIQGSGNHYHACAADDDFDTDFDPSILPP
  PDPWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDRMEGWCQQMEREERENNLPEDILGKIRTAVGSA
  QLLMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDMLQLSIENISMKFDELHQLKANNWKQMDPLDKK
  ERRAPPPVPKKPAKGPAPLIRERSLESSQRQEARKRLMAAKRAASVRQNSATESAESIEIYIPEAQTRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242763
• ORF Size: 2097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242763.1, NP_001229692.1
• RefSeq Size: 5328 bp
• RefSeq ORF: 2100 bp
• Locus ID: 9229
• UniProt ID: O14490
• Cytogenetics: 18p11.31
• Gene Summary: Part of the postsynaptic scaffold in neuronal cells.[UniProtKB/Swiss-Prot Function]