CD6 (NM_001254750) Human Tagged ORF Clone

CAT#: RG234529

CD6 (tGFP-tagged) - Homo sapiens CD6 molecule (CD6), transcript variant 2

Product Images

Circular map for RG234529

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CD6
• Synonyms: TP120
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG234529 representing NM_001254750
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTCTTCTTCGGGATCACTGGATTGCTGACGGCAGCCCTCTCAGGTCATCCATCTCCAGCCCCAC
  CTGACCAGCTCAACACCAGCAGTGCAGAGAGTGAGCTCTGGGAGCCAGGGGAGCGGCTTCCGGTCCGTCT
  GACAAACGGGAGCAGCAGCTGCAGCGGGACGGTGGAGGTGCGGCTCGAGGCGTCCTGGGAGCCCGCGTGC
  GGGGCGCTCTGGGACAGCCGCGCCGCCGAGGCCGTGTGCCGAGCACTGGGCTGCGGCGGGGCGGAGGCCG
  CCTCTCAGCTCGCCCCGCCGACCCCTGAGCTGCCGCCCCCGCCTGCAGCCGGGAACACCAGCGTAGCAGC
  TAATGCCACTCTGGCCGGGGCGCCCGCCCTCCTGTGCAGCGGCGCCGAGTGGCGGCTCTGCGAGGTGGTG
  GAGCACGCGTGCCGCAGCGACGGGAGGCGGGCCCGTGTCACCTGTGCAGAGAACCGCGCGCTGCGCCTGG
  TGGACGGTGGCGGCGCCTGCGCCGGCCGCGTGGAGATGCTGGAGCATGGCGAGTGGGGATCAGTGTGCGA
  TGACACTTGGGACCTGGAGGACGCCCACGTGGTGTGCAGGCAACTGGGCTGCGGCTGGGCAGTCCAGGCC
  CTGCCCGGCTTGCACTTCACGCCCGGCCGCGGGCCTATCCACCGGGACCAGGTGAACTGCTCGGGGGCCG
  AAGCTTACCTGTGGGACTGCCCGGGGCTGCCAGGACAGCACTACTGCGGCCACAAAGAGGACGCGGGCGC
  GGTGTGCTCAGAGCACCAGTCCTGGCGCCTGACAGGGGGCGCTGACCGCTGCGAGGGGCAGGTGGAGGTA
  CACTTCCGAGGGGTCTGGAACACAGTGTGTGACAGTGAGTGGTACCCATCGGAGGCCAAGGTGCTCTGCC
  AGTCCTTGGGCTGTGGAACTGCGGTTGAGAGGCCCAAGGGGCTGCCCCACTCCTTGTCCGGCAGGATGTA
  CTACTCATGCAATGGGGAGGAGCTCACCCTCTCCAACTGCTCCTGGCGGTTCAACAACTCCAACCTCTGC
  AGCCAGTCGCTGGCAGCCAGGGTCCTCTGCTCAGCTTCCCGGAGTTTGCACAATCTGTCCACTCCCGAAG
  TCCCTGCAAGTGTTCAGACAGTCACTATAGAATCTTCTGTGACAGTGAAAATAGAGAACAAGGAATCTCG
  GGAGCTAATGCTCCTCATCCCCTCCATCGTTCTGGGAATTCTCCTCCTTGGCTCCCTCATCTTCATAGCC
  TTCATCCTCTTGAGAATTAAAGGAAAATATGTTTTCATGCTGCCCATCCAGGTCCAGGCCCCGCCCCCTG
  AGGACTCAGACTCTGGCTCGGACTCAGACTATGAGCACTATGACTTCAGCGCCCAGCCTCCTGTGGCCCT
  GACCACCTTCTACAATTCCCAGCGGCATCGGGTCACAGATGAGGAGGTCCAGCAAAGCAGGTTCCAGATG
  CCACCCTTGGAGGAAGGACTTGAAGAGTTGCATGCCTCCCACATCCCAACTGCCAACCCTGGACACTGCA
  TTACAGACCCGCCATCCCTGGGCCCTCAGTATCACCCGAGGAGCAACAGTGAGTCGAGCACCTCTTCAGG
  GGAGGATTACTGCAATAGTCCCAAAAGCAAGCTGCCTCCATGGAACCCCCAGGTGTTTTCTTCAGAGAGG
  AGTTCCTTCCTGGAGCAGCCCCCAAACTTGGAGCTGGCCGGCACCCAGCCAGCCTTTTCAGGGTCCCCCA
  GCCCTCAGCCTGACTCCACCGACAACGATGACTACGATGACATCAGCGCAGCC
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG234529 representing NM_001254750
  Red=Cloning site Green=Tags(s)
  
  MWLFFGITGLLTAALSGHPSPAPPDQLNTSSAESELWEPGERLPVRLTNGSSSCSGTVEVRLEASWEPAC
  GALWDSRAAEAVCRALGCGGAEAASQLAPPTPELPPPPAAGNTSVAANATLAGAPALLCSGAEWRLCEVV
  EHACRSDGRRARVTCAENRALRLVDGGGACAGRVEMLEHGEWGSVCDDTWDLEDAHVVCRQLGCGWAVQA
  LPGLHFTPGRGPIHRDQVNCSGAEAYLWDCPGLPGQHYCGHKEDAGAVCSEHQSWRLTGGADRCEGQVEV
  HFRGVWNTVCDSEWYPSEAKVLCQSLGCGTAVERPKGLPHSLSGRMYYSCNGEELTLSNCSWRFNNSNLC
  SQSLAARVLCSASRSLHNLSTPEVPASVQTVTIESSVTVKIENKESRELMLLIPSIVLGILLLGSLIFIA
  FILLRIKGKYVFMLPIQVQAPPPEDSDSGSDSDYEHYDFSAQPPVALTTFYNSQRHRVTDEEVQQSRFQM
  PPLEEGLEELHASHIPTANPGHCITDPPSLGPQYHPRSNSESSTSSGEDYCNSPKSKLPPWNPQVFSSER
  SSFLEQPPNLELAGTQPAFSGSPSPQPDSTDNDDYDDISAA
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001254750
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001254750.2
• RefSeq Size: 3101 bp
• RefSeq ORF: 1806 bp
• Locus ID: 923
• UniProt ID: P30203
• Cytogenetics: 11q12.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]