CD99L2 (NM_001242614) Human Tagged ORF Clone

CAT#: RG233608

CD99L2 (tGFP-tagged) - Homo sapiens CD99 molecule-like 2 (CD99L2), transcript variant 5

Product Images

Circular map for RG233608

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CD99L2
• Synonyms: CD99B; MIC2L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233608 representing NM_001242614
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCTGGCGCTCGGCGTTCCTTGTCTGCCTCGCTTTCTCCTTGGCCACCCTGGTCCAGCGAGGAT
  CTGGGGACTTTGATGATTTTAACCTGGAGGATGCAGTGAAAGAAACTTCCTCAGTAAAGCAGCCATGGGA
  CCACACCACCACCACCACAACCAATAGGCCAGGAACCACCAGAGCTCCGGCAAAACCTCCAGGGCCCACT
  GAAGGTAGTGGATTGGACTTGGCTGATGCTTTGGATGATCAAGATGATGGCCGCAGGAAACCGGGTATAG
  GAGGAAGAGAGAGATGGAACCATGTAACCACCACGACCAAGAGGCCAGTAACCACCAGAGCTCCAGCAAA
  TACTTTAGGAAATGATTTTGACTTGGCTGATGCCCTGGATGATCGAAATGATCGAGATGATGGCCGCAGG
  AAACCAATTGCTGGAGGAGGAGGTTTTTCAGACAAGGATCTTGAAGACATAGTAGGGGGTGGAGAATACA
  AACCTGACAAGGGTAAAGGTGATGGCCGGTACGGCAGCAATGACGACCCTGGATCTGGCATGGTGGCAGA
  GCCTGGCACCATTGCCGGGGTGGCCAGCGCCCTGGCCATGGCCCTCATCGGTGCCGTCTCCAGCTACATC
  TCCTACCAGCAGAAGAAGTTCTGCTTCAGCATTCAGCATGCAGCAGCAGGTCAAGAGGGTCTCAACGCAG
  ACTACGTGAAGGGAGAGAACCTGGAAGCCGTGGTATGTGAGGAACCCCAAGTGAAATACTCCACGTTGCA
  CACGCAGTCTGCAGAGCCGCCGCCGCCGCCCGAACCAGCCCGGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233608 representing NM_001242614
  Red=Cloning site Green=Tags(s)
  
  MVAWRSAFLVCLAFSLATLVQRGSGDFDDFNLEDAVKETSSVKQPWDHTTTTTTNRPGTTRAPAKPPGPT
  EGSGLDLADALDDQDDGRRKPGIGGRERWNHVTTTTKRPVTTRAPANTLGNDFDLADALDDRNDRDDGRR
  KPIAGGGGFSDKDLEDIVGGGEYKPDKGKGDGRYGSNDDPGSGMVAEPGTIAGVASALAMALIGAVSSYI
  SYQQKKFCFSIQHAAAGQEGLNADYVKGENLEAVVCEEPQVKYSTLHTQSAEPPPPPEPARI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001242614
• ORF Size: 816 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001242614.1, NP_001229543.1
• RefSeq Size: 3754 bp
• RefSeq ORF: 819 bp
• Locus ID: 83692
• UniProt ID: Q8TCZ2
• Cytogenetics: Xq28
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a cell-surface protein that is similar to CD99. A similar protein in mouse functions as an adhesion molecule during leukocyte extravasation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]