ATP2C1 (NM_001199180) Human Tagged ORF Clone

CAT#: RG233189

ATP2C1 (tGFP-tagged) - Homo sapiens ATPase, Ca++ transporting, type 2C, member 1 (ATP2C1), transcript variant 6

Product Images

Circular map for RG233189

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP2C1
• Synonyms: ATP2C1A; BCPM; HHD; hSPCA1; PMR1; SPCA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233189 representing NM_001199180
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAGTCTGTTGCCTCCATCTAGATTCTCTTATTTCAAAAAATATCCTCTCCATGCAATTAGGAGAT
  ATTTATCGACGCTGAGAAACCAAAGAGCCGAAGAACAGGTTGCACGTTTTCAAAAAATACCTAATGGTGA
  AAATGAGACAATGATTCCTGTATTGACATCAAAAAAAGCAAGTGAATTACCAGTCAGTGAAGTTGCAAGC
  ATTCTCCAAGCTGATCTTCAGAATGGTCTAAACAAATGTGAAGTTAGTCATAGGCGAGCCTTTCATGGCT
  GGAATGAGTTTGATATTAGTGAAGATGAGCCACTGTGGAAGAAGTATATTTCTCAGTTTAAAAATCCCCT
  TATTATGCTGCTTCTGGCTTCTGCAGTCATCAGTGTTTTAATGCATCAGTTTGATGATGCCGTCAGTATC
  ACTGTGGCAATACTTATCGTTGTTACAGTTGCCTTTGTTCAGGAATATCGTTCAGAAAAATCTCTTGAAG
  AATTGAGTAAACTTGTGCCACCAGAATGCCATTGTGTGCGTGAAGGAAAATTGGAGCATACACTTGCCCG
  AGACTTGGTTCCAGGTGATACAGTTTGCCTTTCTGTTGGGGATAGAGTTCCTGCTGACTTACGCTTGTTT
  GAGGCTGTGGATCTTTCCATTGATGAGTCCAGCTTGACAGGTGAGACAACGCCTTGTTCTAAGGTGACAG
  CTCCTCAGCCAGCTGCAACTAATGGAGATCTTGCATCGAGAAGTAACATTGCCTTTATGGGAACACTGGT
  CAGATGTGGCAAAGCAAAGGGTGTTGTCATTGGAACAGGAGAAAATTCTGAATTTGGGGAGGTTTTTAAA
  ATGATGCAAGCAGAAGAGGCACCAAAAACCCCTCTGCAGAAGAGCATGGACCTCTTAGGAAAACAACTTT
  CCTTTTACTCCTTTGGTATAATAGGAATCATCATGTTGGTTGGCTGGTTACTGGGAAAAGATATCCTGGA
  AATGTTTACTATTAGTGTAAGTTTGGCTGTAGCAGCAATTCCTGAAGGTCTCCCCATTGTGGTCACAGTG
  ACGCTAGCTCTTGGTGTTATGAGAATGGTGAAGAAAAGGGCCATTGTGAAAAAGCTGCCTATTGTTGAAA
  CTCTGGGCTGCTGTAATGTGATTTGTTCAGATAAAACTGGAACACTGACGAAGAATGAAATGACTGTTAC
  TCACATATTTACTTCAGATGGTCTGCATGCTGAGGTTACTGGAGTTGGCTATAATCAATTTGGGGAAGTG
  ATTGTTGATGGTGATGTTGTTCATGGATTCTATAACCCAGCTGTTAGCAGAATTGTTGAGGCGGGCTGTG
  TGTGCAATGATGCTGTAATTAGAAACAATACTCTAATGGGGAAGCCAACAGAAGGGGCCTTAATTGCTCT
  TGCAATGAAGATGGGTCTTGATGGACTTCAACAAGACTACATCAGAAAAGCTGAATACCCTTTTAGCTCT
  GAGCAAAAGTGGATGGCTGTTAAGTGTGTACACCGAACACAGCAGGACAGACCAGAGATTTGTTTTATGA
  AAGGTGCTTACGAACAAGTAATTAAGTACTGTACTACATACCAGAGCAAAGGGCAGACCTTGACACTTAC
  TCAGCAGCAGAGAGATGTGTACCAACAAGAGAAGGCACGCATGGGCTCAGCGGGACTCAGAGTTCTTGCT
  TTGGCTTCTGGTCCTGAACTGGGACAGCTGACATTTCTTGGCTTGGTGGGAATCATTGATCCACCTAGAA
  CTGGTGTGAAAGAAGCTGTTACAACACTCATTGCCTCAGGAGTATCAATAAAAATGATTACTGGAGATTC
  ACAGGAGACTGCAGTTGCAATCGCCAGTCGTCTGGGATTGTATTCCAAAACTTCCCAGTCAGTCTCAGGA
  GAAGAAATAGATGCAATGGATGTTCAGCAGCTTTCACAAATAGTACCAAAGGTTGCAGTATTTTACAGAG
  CTAGCCCAAGGCACAAGATGAAAATTATTAAGTCGCTACAGAAGAACGGTTCAGTTGTAGCCATGACAGG
  AGATGGAGTAAATGATGCAGTTGCTCTGAAGGCTGCAGACATTGGAGTTGCGATGGGCCAGACTGGTACA
  GATGTTTGCAAAGAGGCAGCAGACATGATCCTAGTGGATGATGATTTTCAAACCATAATGTCTGCAATCG
  AAGAGGGTAAAGGGATTTATAATAACATTAAAAATTTCGTTAGATTCCAGCTGAGCACGAGTATAGCAGC
  ATTAACTTTAATCTCATTGGCTACATTAATGAACTTTCCTAATCCTCTCAATGCCATGCAGATTTTGTGG
  ATCAATATTATTATGGATGGACCCCCAGCTCAGAGCCTTGGAGTAGAACCAGTGGATAAAGATGTCATTC
  GTAAACCTCCTCGCAACTGGAAAGACAGCATTTTGACTAAAAACTTGATACTTAAAATACTTGTTTCATC
  AATAATCATTGTTTGTGGGACTTTGTTTGTCTTCTGGCGTGAGCTACGAGACAATGTGATTACACCTCGA
  GACACAACAATGACCTTCACATGCTTTGTGTTTTTTGACATGTTCAATGCACTAAGTTCCAGATCCCAGA
  CCAAGTCTGTGTTTGAGATTGGACTCTGCAGTAATAGAATGTTTTGCTATGCAGTTCTTGGATCCATCAT
  GGGACAATTACTAGTTATTTACTTTCCTCCGCTTCAGAAGGTTTTTCAGACTGAGAGCCTAAGCATACTG
  GATCTGTTGTTTCTTTTGGGTCTCACCTCATCAGTGTGCATAGTGGCAGAAATTATAAAGAAGGTTGAAA
  GGAGCAGGGAAAAGATCCAGAAGCATGTCTGGCTCTGGGAGAGGAGTGGACAGCAGCTGGTTGAGATACA
  TCCCCATCTGGAGACAGGACTGCCACTGACAGAAGATGTGAGCTGTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233189 representing NM_001199180
  Red=Cloning site Green=Tags(s)
  
  MDSLLPPSRFSYFKKYPLHAIRRYLSTLRNQRAEEQVARFQKIPNGENETMIPVLTSKKASELPVSEVAS
  ILQADLQNGLNKCEVSHRRAFHGWNEFDISEDEPLWKKYISQFKNPLIMLLLASAVISVLMHQFDDAVSI
  TVAILIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLF
  EAVDLSIDESSLTGETTPCSKVTAPQPAATNGDLASRSNIAFMGTLVRCGKAKGVVIGTGENSEFGEVFK
  MMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFTISVSLAVAAIPEGLPIVVTV
  TLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNEMTVTHIFTSDGLHAEVTGVGYNQFGEV
  IVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSS
  EQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYQSKGQTLTLTQQQRDVYQQEKARMGSAGLRVLA
  LASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQETAVAIASRLGLYSKTSQSVSG
  EEIDAMDVQQLSQIVPKVAVFYRASPRHKMKIIKSLQKNGSVVAMTGDGVNDAVALKAADIGVAMGQTGT
  DVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRFQLSTSIAALTLISLATLMNFPNPLNAMQILW
  INIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSILTKNLILKILVSSIIIVCGTLFVFWRELRDNVITPR
  DTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSNRMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSIL
  DLLFLLGLTSSVCIVAEIIKKVERSREKIQKHVWLWERSGQQLVEIHPHLETGLPLTEDVSCV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199180
• ORF Size: 2919 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199180.2
• RefSeq Size: 3702 bp
• RefSeq ORF: 2922 bp
• Locus ID: 27032
• UniProt ID: P98194
• Cytogenetics: 3q22.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]