ATP2C1 (NM_001199183) Human Tagged ORF Clone

CAT#: RG233177

ATP2C1 (tGFP-tagged) - Homo sapiens ATPase, Ca++ transporting, type 2C, member 1 (ATP2C1), transcript variant 9

Product Images

Circular map for RG233177

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP2C1
• Synonyms: ATP2C1A; BCPM; HHD; hSPCA1; PMR1; SPCA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233177 representing NM_001199183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCTGTATTGACATCAAAAAAAGCAAGTGAATTACCAGTCAGTGAAGTTGCAAGCATTCTCCAAG
  CTGATCTTCAGAATGGTCTAAACAAATGTGAAGTTAGTCATAGGCGAGCCTTTCATGGCTGGAATGAGTT
  TGATATTAGTGAAGATGAGCCACTGTGGAAGAAGTATATTTCTCAGTTTAAAAATCCCCTTATTATGCTG
  CTTCTGGCTTCTGCAGTCATCAGTGTTTTAATGCATCAGTTTGATGATGCCGTCAGTATCACTGTGGCAA
  TACTTATCGTTGTTACAGTTGCCTTTGTTCAGGAATATCGTTCAGAAAAATCTCTTGAAGAATTGAGTAA
  ACTTGTGCCACCAGAATGCCATTGTGTGCGTGAAGGAAAATTGGAGCATACACTTGCCCGAGACTTGGTT
  CCAGGTGATACAGTTTGCCTTTCTGTTGGGGATAGAGTTCCTGCTGACTTACGCTTGTTTGAGGCTGTGG
  ATCTTTCCATTGATGAGTCCAGCTTGACAGGTGAGACAACGCCTTGTTCTAAGGTGACAGCTCCTCAGCC
  AGCTGCAACTAATGGAGATCTTGCATCGAGAAGTAACATTGCCTTTATGGGAACACTGGTCAGATGTGGC
  AAAGCAAAGGGTGTTGTCATTGGAACAGGAGAAAATTCTGAATTTGGGGAGGTTTTTAAAATGATGCAAG
  CAGAAGAGGCACCAAAAACCCCTCTGCAGAAGAGCATGGACCTCTTAGGAAAACAACTTTCCTTTTACTC
  CTTTGGTATAATAGGAATCATCATGTTGGTTGGCTGGTTACTGGGAAAAGATATCCTGGAAATGTTTACT
  ATTAGTGTAAGTTTGGCTGTAGCAGCAATTCCTGAAGGTCTCCCCATTGTGGTCACAGTGACGCTAGCTC
  TTGGTGTTATGAGAATGGTGAAGAAAAGGGCCATTGTGAAAAAGCTGCCTATTGTTGAAACTCTGGGCTG
  CTGTAATGTGATTTGTTCAGATAAAACTGGAACACTGACGAAGAATGAAATGACTGTTACTCACATATTT
  ACTTCAGATGGTCTGCATGCTGAGGTTACTGGAGTTGGCTATAATCAATTTGGGGAAGTGATTGTTGATG
  GTGATGTTGTTCATGGATTCTATAACCCAGCTGTTAGCAGAATTGTTGAGGCGGGCTGTGTGTGCAATGA
  TGCTGTAATTAGAAACAATACTCTAATGGGGAAGCCAACAGAAGGGGCCTTAATTGCTCTTGCAATGAAG
  ATGGGTCTTGATGGACTTCAACAAGACTACATCAGAAAAGCTGAATACCCTTTTAGCTCTGAGCAAAAGT
  GGATGGCTGTTAAGTGTGTACACCGAACACAGCAGGACAGACCAGAGATTTGTTTTATGAAAGGTGCTTA
  CGAACAAGTAATTAAGTACTGTACTACATACCAGAGCAAAGGGCAGACCTTGACACTTACTCAGCAGCAG
  AGAGATGTGTACCAACAAGAGAAGGCACGCATGGGCTCAGCGGGACTCAGAGTTCTTGCTTTGGCTTCTG
  GTCCTGAACTGGGACAGCTGACATTTCTTGGCTTGGTGGGAATCATTGATCCACCTAGAACTGGTGTGAA
  AGAAGCTGTTACAACACTCATTGCCTCAGGAGTATCAATAAAAATGATTACTGGAGATTCACAGGAGACT
  GCAGTTGCAATCGCCAGTCGTCTGGGATTGTATTCCAAAACTTCCCAGTCAGTCTCAGGAGAAGAAATAG
  ATGCAATGGATGTTCAGCAGCTTTCACAAATAGTACCAAAGGTTGCAGTATTTTACAGAGCTAGCCCAAG
  GCACAAGATGAAAATTATTAAGTCGCTACAGAAGAACGGTTCAGTTGTAGCCATGACAGGAGATGGAGTA
  AATGATGCAGTTGCTCTGAAGGCTGCAGACATTGGAGTTGCGATGGGCCAGACTGGTACAGATGTTTGCA
  AAGAGGCAGCAGACATGATCCTAGTGGATGATGATTTTCAAACCATAATGTCTGCAATCGAAGAGGGTAA
  AGGGATTTATAATAACATTAAAAATTTCGTTAGATTCCAGCTGAGCACGAGTATAGCAGCATTAACTTTA
  ATCTCATTGGCTACATTAATGAACTTTCCTAATCCTCTCAATGCCATGCAGATTTTGTGGATCAATATTA
  TTATGGATGGACCCCCAGCTCAGAGCCTTGGAGTAGAACCAGTGGATAAAGATGTCATTCGTAAACCTCC
  TCGCAACTGGAAAGACAGCATTTTGACTAAAAACTTGATACTTAAAATACTTGTTTCATCAATAATCATT
  GTTTGTGGGACTTTGTTTGTCTTCTGGCGTGAGCTACGAGACAATGTGATTACACCTCGAGACACAACAA
  TGACCTTCACATGCTTTGTGTTTTTTGACATGTTCAATGCACTAAGTTCCAGATCCCAGACCAAGTCTGT
  GTTTGAGATTGGACTCTGCAGTAATAGAATGTTTTGCTATGCAGTTCTTGGATCCATCATGGGACAATTA
  CTAGTTATTTACTTTCCTCCGCTTCAGAAGGTTTTTCAGACTGAGAGCCTAAGCATACTGGATCTGTTGT
  TTCTTTTGGGTCTCACCTCATCAGTGTGCATAGTGGCAGAAATTATAAAGAAGGTTGAAAGGAGCAGGGA
  AAAGATCCAGAAGCATGTCTGGCTCTGGGAGAGGAGTGGACAGCAGCTGGTTGAGATACATCCCCATCTG
  GAGACAGGACTGCCACTGACAGAAGATGTGAGCTGTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233177 representing NM_001199183
  Red=Cloning site Green=Tags(s)
  
  MIPVLTSKKASELPVSEVASILQADLQNGLNKCEVSHRRAFHGWNEFDISEDEPLWKKYISQFKNPLIML
  LLASAVISVLMHQFDDAVSITVAILIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLV
  PGDTVCLSVGDRVPADLRLFEAVDLSIDESSLTGETTPCSKVTAPQPAATNGDLASRSNIAFMGTLVRCG
  KAKGVVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFT
  ISVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNEMTVTHIF
  TSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKPTEGALIALAMK
  MGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYQSKGQTLTLTQQQ
  RDVYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQET
  AVAIASRLGLYSKTSQSVSGEEIDAMDVQQLSQIVPKVAVFYRASPRHKMKIIKSLQKNGSVVAMTGDGV
  NDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRFQLSTSIAALTL
  ISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSILTKNLILKILVSSIII
  VCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSNRMFCYAVLGSIMGQL
  LVIYFPPLQKVFQTESLSILDLLFLLGLTSSVCIVAEIIKKVERSREKIQKHVWLWERSGQQLVEIHPHL
  ETGLPLTEDVSCV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199183
• ORF Size: 2769 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199183.2
• RefSeq Size: 3575 bp
• RefSeq ORF: 2772 bp
• Locus ID: 27032
• UniProt ID: P98194
• Cytogenetics: 3q22.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]