ATP2C1 (NM_001199179) Human Tagged ORF Clone

CAT#: RG233175

ATP2C1 (tGFP-tagged) - Homo sapiens ATPase, Ca++ transporting, type 2C, member 1 (ATP2C1), transcript variant 5

Product Images

Circular map for RG233175

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP2C1
• Synonyms: ATP2C1A; BCPM; HHD; hSPCA1; PMR1; SPCA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233175 representing NM_001199179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTTGCACGTTTTCAAAAAATACCTAATGGTGAAAATGAGACAATGATTCCTGTATTGACATCAA
  AAAAAGCAAGTGAATTACCAGTCAGTGAAGTTGCAAGCATTCTCCAAGCTGATCTTCAGAATGGTCTAAA
  CAAATGTGAAGTTAGTCATAGGCGAGCCTTTCATGGCTGGAATGAGTTTGATATTAGTGAAGATGAGCCA
  CTGTGGAAGAAGTATATTTCTCAGTTTAAAAATCCCCTTATTATGCTGCTTCTGGCTTCTGCAGTCATCA
  GTGTTTTAATGCATCAGTTTGATGATGCCGTCAGTATCACTGTGGCAATACTTATCGTTGTTACAGTTGC
  CTTTGTTCAGGAATATCGTTCAGAAAAATCTCTTGAAGAATTGAGTAAACTTGTGCCACCAGAATGCCAT
  TGTGTGCGTGAAGGAAAATTGGAGCATACACTTGCCCGAGACTTGGTTCCAGGTGATACAGTTTGCCTTT
  CTGTTGGGGATAGAGTTCCTGCTGACTTACGCTTGTTTGAGGCTGTGGATCTTTCCATTGATGAGTCCAG
  CTTGACAGGTGAGACAACGCCTTGTTCTAAGGTGACAGCTCCTCAGCCAGCTGCAACTAATGGAGATCTT
  GCATCGAGAAGTAACATTGCCTTTATGGGAACACTGGTCAGATGTGGCAAAGCAAAGGGTGTTGTCATTG
  GAACAGGAGAAAATTCTGAATTTGGGGAGGTTTTTAAAATGATGCAAGCAGAAGAGGCACCAAAAACCCC
  TCTGCAGAAGAGCATGGACCTCTTAGGAAAACAACTTTCCTTTTACTCCTTTGGTATAATAGGAATCATC
  ATGTTGGTTGGCTGGTTACTGGGAAAAGATATCCTGGAAATGTTTACTATTAGTGTAAGTTTGGCTGTAG
  CAGCAATTCCTGAAGGTCTCCCCATTGTGGTCACAGTGACGCTAGCTCTTGGTGTTATGAGAATGGTGAA
  GAAAAGGGCCATTGTGAAAAAGCTGCCTATTGTTGAAACTCTGGGCTGCTGTAATGTGATTTGTTCAGAT
  AAAACTGGAACACTGACGAAGAATGAAATGACTGTTACTCACATATTTACTTCAGATGGTCTGCATGCTG
  AGGTTACTGGAGTTGGCTATAATCAATTTGGGGAAGTGATTGTTGATGGTGATGTTGTTCATGGATTCTA
  TAACCCAGCTGTTAGCAGAATTGTTGAGGCGGGCTGTGTGTGCAATGATGCTGTAATTAGAAACAATACT
  CTAATGGGGAAGCCAACAGAAGGGGCCTTAATTGCTCTTGCAATGAAGATGGGTCTTGATGGACTTCAAC
  AAGACTACATCAGAAAAGCTGAATACCCTTTTAGCTCTGAGCAAAAGTGGATGGCTGTTAAGTGTGTACA
  CCGAACACAGCAGGACAGACCAGAGATTTGTTTTATGAAAGGTGCTTACGAACAAGTAATTAAGTACTGT
  ACTACATACCAGAGCAAAGGGCAGACCTTGACACTTACTCAGCAGCAGAGAGATGTGTACCAACAAGAGA
  AGGCACGCATGGGCTCAGCGGGACTCAGAGTTCTTGCTTTGGCTTCTGGTCCTGAACTGGGACAGCTGAC
  ATTTCTTGGCTTGGTGGGAATCATTGATCCACCTAGAACTGGTGTGAAAGAAGCTGTTACAACACTCATT
  GCCTCAGGAGTATCAATAAAAATGATTACTGGAGATTCACAGGAGACTGCAGTTGCAATCGCCAGTCGTC
  TGGGATTGTATTCCAAAACTTCCCAGTCAGTCTCAGGAGAAGAAATAGATGCAATGGATGTTCAGCAGCT
  TTCACAAATAGTACCAAAGGTTGCAGTATTTTACAGAGCTAGCCCAAGGCACAAGATGAAAATTATTAAG
  TCGCTACAGAAGAACGGTTCAGTTGTAGCCATGACAGGAGATGGAGTAAATGATGCAGTTGCTCTGAAGG
  CTGCAGACATTGGAGTTGCGATGGGCCAGACTGGTACAGATGTTTGCAAAGAGGCAGCAGACATGATCCT
  AGTGGATGATGATTTTCAAACCATAATGTCTGCAATCGAAGAGGGTAAAGGGATTTATAATAACATTAAA
  AATTTCGTTAGATTCCAGCTGAGCACGAGTATAGCAGCATTAACTTTAATCTCATTGGCTACATTAATGA
  ACTTTCCTAATCCTCTCAATGCCATGCAGATTTTGTGGATCAATATTATTATGGATGGACCCCCAGCTCA
  GAGCCTTGGAGTAGAACCAGTGGATAAAGATGTCATTCGTAAACCTCCTCGCAACTGGAAAGACAGCATT
  TTGACTAAAAACTTGATACTTAAAATACTTGTTTCATCAATAATCATTGTTTGTGGGACTTTGTTTGTCT
  TCTGGCGTGAGCTACGAGACAATGTGATTACACCTCGAGACACAACAATGACCTTCACATGCTTTGTGTT
  TTTTGACATGTTCAATGCACTAAGTTCCAGATCCCAGACCAAGTCTGTGTTTGAGATTGGACTCTGCAGT
  AATAGAATGTTTTGCTATGCAGTTCTTGGATCCATCATGGGACAATTACTAGTTATTTACTTTCCTCCGC
  TTCAGAAGGTTTTTCAGACTGAGAGCCTAAGCATACTGGATCTGTTGTTTCTTTTGGGTCTCACCTCATC
  AGTGTGCATAGTGGCAGAAATTATAAAGAAGGTTGAAAGGAGCAGGGAAAAGATCCAGAAGCATGTTAGT
  TCGACATCATCATCTTTTCTTGAAGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233175 representing NM_001199179
  Red=Cloning site Green=Tags(s)
  
  MKVARFQKIPNGENETMIPVLTSKKASELPVSEVASILQADLQNGLNKCEVSHRRAFHGWNEFDISEDEP
  LWKKYISQFKNPLIMLLLASAVISVLMHQFDDAVSITVAILIVVTVAFVQEYRSEKSLEELSKLVPPECH
  CVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSIDESSLTGETTPCSKVTAPQPAATNGDL
  ASRSNIAFMGTLVRCGKAKGVVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGII
  MLVGWLLGKDILEMFTISVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSD
  KTGTLTKNEMTVTHIFTSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNT
  LMGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYC
  TTYQSKGQTLTLTQQQRDVYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLI
  ASGVSIKMITGDSQETAVAIASRLGLYSKTSQSVSGEEIDAMDVQQLSQIVPKVAVFYRASPRHKMKIIK
  SLQKNGSVVAMTGDGVNDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIK
  NFVRFQLSTSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSI
  LTKNLILKILVSSIIIVCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCS
  NRMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSILDLLFLLGLTSSVCIVAEIIKKVERSREKIQKHVS
  STSSSFLEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199179
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199179.2
• RefSeq Size: 5051 bp
• RefSeq ORF: 2760 bp
• Locus ID: 27032
• UniProt ID: P98194
• Cytogenetics: 3q22.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium ions. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]