ODF2 (NM_153440) Human Tagged ORF Clone

CAT#: RG233044

ODF2 (tGFP-tagged) - Homo sapiens outer dense fiber of sperm tails 2 (ODF2), transcript variant 7

Product Images

Circular map for RG233044

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ODF2
• Synonyms: CT134; ODF2/1; ODF2/2; ODF84
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG233044 representing NM_153440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGAAACCGGTACCCTCCTGCCTGCTGGTTTCCATCGTGTGGGAAGAACGGAGTAACGAGTCTCA
  CGCAGAAAAAGGTCTTGAGAGCACCTTGTGGCGCACCCAGTGTAACTGTGACGAAATCTCACAAGCGAGG
  AATGAAAGGGGACACTGTGAATGTGCGGCGGAGTGTCCGGGTGAAAACCAAGAATCCACCTCATTGCCTG
  GAGATCACGCCACCATCTTCAGAAAAGCTGGTCTCAGTGATGCGGTTAAGTGACCTCTCTACAGAAGATG
  ATGACTCAGGTCACTGTAAAATGAACCGTTATGATAAGAAGATTGATAGTCTAATGAATGCGGTTGGTTG
  TCTGAAGTCTGAGGTCAAGATGCAAAAAGGTGAGCGCCAGATGGCCAAAAGGTTCCTGGAGGAACGGAAG
  GAAGAGCTGGAGGAGGTGGCCCACGAACTGGCTGAGACTGAGCACGAGAACACGGTGTTGAGGCACAACA
  TCGAGCGCATGAAGGAGGAGAAGGACTTCACCATACTTCAGAAGAAACACCTACAACAGGAGAAGGAGTG
  CCTCATGTCCAAGCTGGTGGAGGCGGAAATGGATGGGGCTGCGGCTGCCAAGCAGGTCATGGCCTTGAAG
  GATACCATCGGGAAGCTGAAAACGGAGAAACAAATGACCTGCACGGACATCAACACCCTGACAAGGCAGA
  AGGAACTTCTCCTGCAGAAGCTGAGCACATTTGAGGAGACCAACCGCACCCTCCGAGACCTCCTGAGGGA
  ACAGCACTGCAAAGAGGATTCTGAAAGACTAATGGAGCAACAAGGAGCACTGCTGAAACGGCTGGCGGAG
  GCCGACTCAGAGAAAGCGCGCCTGCTGTTACTGCTGCAAGACAAGGACAAGGAGGTGGAAGAGCTCCTTC
  AGGAAATACAATGTGAGAAGGCTCAAGCAAAGACAGCCTCTGAGCTTTCTAAATCCATGGAGTCCATGCG
  TGGGCATTTGCAGGCACAGCTTCGGTCCAAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATTAAGAAT
  CTGGAGCGCAGCGGGAATCAGCATAAGGCAGAAGTGGAGGCCATCATGGAGCAGCTGAAGGAGTTGAAGC
  AGAAGGGAGACCGAGACAAAGAGAGCTTGAAGAAGGCCATCCGAGCCCAGAAGGAGCGAGCCGAGAAGAG
  CGAGGAGTATGCTGAGCAGCTACACGTGCAACTCGCTGACAAGGATCTTTATGTCGCTGAAGCTTTATCC
  ACTCTGGAATCCTGGAGGAGCCGCTACAACCAAGTTGTAAAAGAAAAGGGAGACCTTGAGCTGGAAATTA
  TTGTCCTGAATGACCGGGTAACAGATCTTGTAAACCAACAACAAACCCTGGAGGAGAAGATGCGGGAAGA
  CCGGGATAGCCTGGTGGAGAGACTACACCGTCAGACTGCTGAGTATTCCGCATTCAAGCTGGAGAATGAG
  AGGCTGAAGGCCAGCTTTGCTCCAATGGAGGACAAACTCAACCAGGCACACCTCGAGGTCCAGCAGCTGA
  AGGCCTCAGTGAAGAACTATGAGGGGATGATTGACAACTATAAGAGTCAGGTGATGAAGACCAGATTGGA
  GGCTGATGAAGTAGCTGCCCAGCTAGAACGCTGTGACAAAGAGAACAAGATCCTTAAAGATGAGATGAAC
  AAAGAGATTGAGGCGGCACGAAGGCAGTTCCAGTCTCAGCTGGCTGACCTGCAGCAGCTCCCTGACATCC
  TGAAGATCACGGAGGCGAAGCTGGCTGAGTGCCAAGACCAACTGCAGGGCTATGAGCGGAAGAACATCGA
  CCTCACAGCCATCATATCAGACCTGCGCAGCCGGGTAAGGGACTGGCAGAAAGGGTCCCACGAACTGACC
  CGAGCAGGGGCCCGCATACCAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG233044 representing NM_153440
  Red=Cloning site Green=Tags(s)
  
  MGRNRYPPACWFPSCGKNGVTSLTQKKVLRAPCGAPSVTVTKSHKRGMKGDTVNVRRSVRVKTKNPPHCL
  EITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSEVKMQKGERQMAKRFLEERK
  EELEEVAHELAETEHENTVLRHNIERMKEEKDFTILQKKHLQQEKECLMSKLVEAEMDGAAAAKQVMALK
  DTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCKEDSERLMEQQGALLKRLAE
  ADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQAQLRSKEAENSRLCMQIKN
  LERSGNQHKAEVEAIMEQLKELKQKGDRDKESLKKAIRAQKERAEKSEEYAEQLHVQLADKDLYVAEALS
  TLESWRSRYNQVVKEKGDLELEIIVLNDRVTDLVNQQQTLEEKMREDRDSLVERLHRQTAEYSAFKLENE
  RLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEVAAQLERCDKENKILKDEMN
  KEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAIISDLRSRVRDWQKGSHELT
  RAGARIPR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153440
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153440.1, NP_702918.1
• RefSeq Size: 2171 bp
• RefSeq ORF: 1917 bp
• Locus ID: 4957
• Cytogenetics: 9q34.11
• Gene Summary: The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010]