KLHL3 (NM_001257195) Human Tagged ORF Clone

CAT#: RG232883

KLHL3 (tGFP-tagged) - Homo sapiens kelch-like family member 3 (KLHL3), transcript variant 3

Product Images

Circular map for RG232883

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHL3
• Synonyms: PHA2D
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232883 representing NM_001257195
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAGAGTAAAGCCAAAAAGATAGAAATCAAGGACGTGGATGGGCAGACGCTGAGTAAGCTGATTG
  ACTACATCTATACTGCTGAAATCGAGGTGACTGAAGAGAATGTCCAGGTGCTGCTCCCGGCAGCCAGCTT
  GCTGCAGCTCATGGATGTTCGGCAGAACTGCTGTGACTTCCTGCAGTCTCAGTTGCATCCCACCAATTGC
  CTGGGCATCCGTGCATTTGCAGATGTACACACCTGCACTGACCTTCTGCAGCAGGCCAATGCCTACGCAG
  AGCAGCACTTTCCAGAGGTGATGCTAGGAGAAGAATTTCTTAGCCTGAGTCTGGACCAGGTGTGCAGCTT
  GATATCCAGCGACAAGCTGACCGTTTCTTCAGAAGAGAAGGTGTTTGAAGCTGTGATCTCATGGATCAAT
  TATGAGAAAGAAACCCGTTTAGAGCACATGGCAAAGCTGATGGAACATGTCCGACTTCCTCTCTTACCTA
  GGGACTACCTAGTCCAAACGGTTGAAGAAGAAGCTTTGATAAAGAATAACAACACCTGTAAAGACTTCCT
  CATTGAGGCCATGAAATACCATCTCCTCCCTCTGGATCAGAGACTATTGATTAAGAACCCAAGGACCAAG
  CCCAGGACTCCAGTCAGCCTTCCCAAGGTCATGATTGTGGTTGGCGGCCAGGCACCCAAGGCAATCCGCA
  GTGTGGAGTGCTATGATTTCGAGGAGGACCGGTGGGATCAGATTGCTGAGCTTCCTTCCAGAAGATGCAG
  AGCAGGTGTGGTGTTCATGGCTGGCCACGTGTATGCCGTGGGAGGGTTTAATGGCTCACTGCGGGTGCGG
  ACAGTGGATGTGTATGACGGCGTGAAGGACCAGTGGACGTCCATTGCCAGCATGCAGGAGCGCCGGAGCA
  CACTGGGCGCAGCGGTGCTCAATGACTTGCTCTACGCAGTGGGAGGCTTTGATGGCAGTACTGGCCTAGC
  ATCGGTGGAAGCCTACAGCTACAAGACCAACGAGTGGTTCTTTGTGGCCCCGATGAACACGCGGCGGAGC
  AGTGTGGGTGTGGGCGTTGTGGAGGGGAAGCTATATGCTGTTGGGGGTTATGATGGAGCTTCCCGCCAGT
  GTCTGAGCACTGTGGAGCAGTACAACCCAGCGACCAATGAATGGATATACGTGGCGGACATGAGCACCCG
  CCGCAGTGGCGCAGGGGTTGGAGTGCTTAGCGGACAGCTGTACGCCACAGGTGGGCATGATGGGCCTTTG
  GTGAGGAAGAGCGTTGAGGTTTACGATCCTGGAACAAATACCTGGAAGCAAGTGGCAGACATGAACATGT
  GCCGGCGCAACGCAGGGGTCTGTGCAGTAAATGGGCTCCTGTATGTGGTTGGAGGGGATGATGGATCCTG
  CAACTTGGCTTCGGTGGAGTACTACAATCCTGTCACTGACAAATGGACGCTGCTTCCAACGAACATGAGC
  ACGGGGCGGAGCTATGCAGGTGTTGCCGTGATTCACAAGTCCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232883 representing NM_001257195
  Red=Cloning site Green=Tags(s)
  
  MSESKAKKIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNCCDFLQSQLHPTNC
  LGIRAFADVHTCTDLLQQANAYAEQHFPEVMLGEEFLSLSLDQVCSLISSDKLTVSSEEKVFEAVISWIN
  YEKETRLEHMAKLMEHVRLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPLDQRLLIKNPRTK
  PRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCRAGVVFMAGHVYAVGGFNGSLRVR
  TVDVYDGVKDQWTSIASMQERRSTLGAAVLNDLLYAVGGFDGSTGLASVEAYSYKTNEWFFVAPMNTRRS
  SVGVGVVEGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLSGQLYATGGHDGPL
  VRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPVTDKWTLLPTNMS
  TGRSYAGVAVIHKSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001257195
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001257195.1, NP_001244124.1
• RefSeq Size: 6557 bp
• RefSeq ORF: 1518 bp
• Locus ID: 26249
• UniProt ID: Q9UH77
• Cytogenetics: 5q31.2
• Gene Summary: This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]