TCP11 (NM_001261820) Human Tagged ORF Clone

CAT#: RG232730

TCP11 (tGFP-tagged) - Homo sapiens t-complex 11, testis-specific (TCP11), transcript variant 6

Product Images

Circular map for RG232730

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TCP11
• Synonyms: D6S230E; FPPR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232730 representing NM_001261820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTGTGATTACTACATGGAAGAGAAGGTTTTACCTCCAAGCAGTCTGGAAGGCAAGGTCAAGGAGA
  CAGTGCACAATGCCTTTTGGGACCATCTTAAAGAGCAACTATCAGCAACTCCCCCTGACTTCAGCTGTGC
  TCTTGAACTTCTGAAAGAAATTAAAGAGATCTTGCTATCACTGCTATTACCACGCCAGAACCGCCTGAGA
  ATTGAGATTGAAGAAGCTCTGGACATGGACTTGCTCAAGCAGGAGGCAGAACATGGGGCCCTGAAAGTCC
  TCTATCTCTCTAAGTACGTTCTCAACATGATGGCTTTGCTGTGTGCACCAGTTCGAGATGAAGCAGTGCA
  GAAACTAGAAAACATTACGGATCCTGTTTGGCTACTGAGAGGGATCTTCCAGGTTCTGGGCCGGATGAAA
  ATGGACATGGTGAACTACACTATCCAGAGCCTTCAACCCCACCTGCAGGAACATTCCATTCAGTATGAAC
  GGGCTAAATTCCAGGAACTCCTCAATAAGCAGCCTAGTCTCCTTAATCACACCACCAAATGGCTGACCCA
  AGCAGCAGGAGACCTCACCATGTCACCTCCGACTTGCCCAGACACTTCTGACTCCTCCAGTGTGGCTGGC
  CCCTCTCCCAATGAGGCAGCCAACAACCCAGAGCCCCTCAGCCCCACAATGGTGCTGTGTCAGGGCTTCT
  TGAACCTCCTTCTCTGGGACCTTGAAAATGAAGAGTTCCCTGAGACCCTGCTGATGGACAGAACCCGGCT
  GCAGGAGCTGAAGTCCCAGTTGCACCAGTTAACCGTCATGGCCTCAGTCTTGCTGGTGGCCAGTAGTTTC
  TCCGGCAGTGTTTTGTTTGGCTCACCCCAATTTGTAGATAAACTGAAACGCATAACCAAATCCTTGTTGG
  AAGACTTTCACTCCAGGCCTGAGGAAGCTATACTGACTGTGAGTGAACAGGTATCTCAGGAAATCCATCA
  AAGCCTCAAGAATATGGGCCTTGTTGCTCTAAGCAGTGATAATACAGCATCTCTAATGGGACAGCTCCAG
  AACATTGCCAAGAAGGAGAACTGTGTCTGCAGTGTTATTGATCAGCGGATCCATTTGTTTCTCAAATGCT
  GTTTGGTTCTTGGTGTGCAGCGGTCTCTATTAGACCTTCCTGGAGGCCTTACTCTCATTGAAGCAGAACT
  GGCAGAACTGGGCCAAAAGTTTGTCAACTTGACACATCACAATCAGCAGGTGTTTGGTCCCTACTACACT
  GAGATCCTAAAAACCCTCATTTCCCCAGCCCAGGCACTGGAAACAAAAGTGGAGTCTGTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232730 representing NM_001261820
  Red=Cloning site Green=Tags(s)
  
  MNCDYYMEEKVLPPSSLEGKVKETVHNAFWDHLKEQLSATPPDFSCALELLKEIKEILLSLLLPRQNRLR
  IEIEEALDMDLLKQEAEHGALKVLYLSKYVLNMMALLCAPVRDEAVQKLENITDPVWLLRGIFQVLGRMK
  MDMVNYTIQSLQPHLQEHSIQYERAKFQELLNKQPSLLNHTTKWLTQAAGDLTMSPPTCPDTSDSSSVAG
  PSPNEAANNPEPLSPTMVLCQGFLNLLLWDLENEEFPETLLMDRTRLQELKSQLHQLTVMASVLLVASSF
  SGSVLFGSPQFVDKLKRITKSLLEDFHSRPEEAILTVSEQVSQEIHQSLKNMGLVALSSDNTASLMGQLQ
  NIAKKENCVCSVIDQRIHLFLKCCLVLGVQRSLLDLPGGLTLIEAELAELGQKFVNLTHHNQQVFGPYYT
  EILKTLISPAQALETKVESV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001261820
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001261820.1, NP_001248749.1
• RefSeq Size: 2147 bp
• RefSeq ORF: 1323 bp
• Locus ID: 6954
• UniProt ID: Q8WWU5
• Cytogenetics: 6p21.31
• Gene Summary: Plays a role in the process of sperm capacitation and acrosome reactions. Probable receptor for the putative fertilization-promoting peptide (FPP) at the sperm membrane that may modulate the activity of the adenylyl cyclase cAMP pathway.[UniProtKB/Swiss-Prot Function]