TBC1D7 (NM_001258457) Human Tagged ORF Clone

CAT#: RG232272

TBC1D7 (tGFP-tagged) - Homo sapiens TBC1 domain family, member 7 (TBC1D7), transcript variant 5

Product Images

Circular map for RG232272

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TBC1D7
• Synonyms: MGCPH; PIG51; TBC7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG232272 representing NM_001258457
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAGGACTCTCAGAGAAACTTTCGTTCAGTATATTATGAGAAAGTGGGGTTTCGTGGAGTTGAAG
  AAAAGAAATCATTAGAAATTCTCCTAAAAGATGACCGTCTGGATACTGAGAAACTTTGTACTTTTAGTCA
  GAGGTTCCCTCTCCCGTCCATGTACCGTGCATTGGTATGGAAGGTGCTTCTAGGAATCTTGCCTCCACAC
  CACGAGTCCCATGCCAAGGTGATGATGTATCGTAAGGAGCAGTACTTGGATGTCCTTCATGCCCTGAAAG
  TCGTTCGCTTTGTTAGTGATGCCACACCTCAGGCTGAAGTCTATCTCCGCATGTATCAGCTGGAGTCTGG
  GAAGTTACCTCGAAGTCCCTCTTTTCCACTGCCAAAAGCGTTTGAACAATACTTGAATCTGGAAGATGGC
  AGACTGCTGACTCATCTGAGGATGTGTTCCGCGGCGCCCAAACTTCCTTATGATCTCTGGTTCAAGAGGT
  GCTTTGCGGGATGTTTGCCTGAATCCAGTTTACAGAGGGTTTGGGATAAAGTTGTGAGTGGATCCTGTAA
  GATCCTAGTTTTTGTAGCTGTCGAAATTTTATTAACCTTTAAAATAAAAGTTATGGCACTGAACAGTGCA
  GAGAAGATAACAAAGTTTCTGGAAAATATTCCCCAGGACAGCTCAGACGCGATCGTGAGCAAGGCCATTG
  ACTTGTGGCACAAACACTGTGGGACCCCGGTCCATTCAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG232272 representing NM_001258457
  Red=Cloning site Green=Tags(s)
  
  MTEDSQRNFRSVYYEKVGFRGVEEKKSLEILLKDDRLDTEKLCTFSQRFPLPSMYRALVWKVLLGILPPH
  HESHAKVMMYRKEQYLDVLHALKVVRFVSDATPQAEVYLRMYQLESGKLPRSPSFPLPKAFEQYLNLEDG
  RLLTHLRMCSAAPKLPYDLWFKRCFAGCLPESSLQRVWDKVVSGSCKILVFVAVEILLTFKIKVMALNSA
  EKITKFLENIPQDSSDAIVSKAIDLWHKHCGTPVHSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258457
• ORF Size: 741 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258457.2, NP_001245386.1
• RefSeq Size: 1042 bp
• RefSeq ORF: 744 bp
• Locus ID: 51256
• UniProt ID: Q9P0N9
• Cytogenetics: 6p24.1
• Gene Summary: This gene encodes a member of the TBC-domain containing protein family. The encoded protein functions as a subunit of the tuberous sclerosis TSC1-TSC2 complex which plays a role in the regulation of cellular growth and differentiation. Mutations in this gene have been associated with autosomal recessive megalencephaly. Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between this locus and downstream LOC100130357. [provided by RefSeq, Jan 2016]