ODF2 (NM_153436) Human Tagged ORF Clone

CAT#: RG231359

ODF2 (tGFP-tagged) - Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 4

Product Images

Circular map for RG231359

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ODF2
• Synonyms: CT134; ODF2/1; ODF2/2; ODF84
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231359 representing NM_153436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCAGCAAGGACCTCATCAGAATAGGTTTCCATCGTGTGGGAAGAACGGAGTAACGAGTCTCA
  CGCAGAAAAAGGTCTTGAGAGCACCTTGTGGCGCACCCAGTGTAACTGTGACGAAATCTCACAAGCGAGG
  AATGAAAGGGGACACTGTGAATGTGCGGCGGAGTGTCCGGGTGAAAACCAAGGTACCTTGGATGCCCCCT
  GGAAAATCATCTGCCCGGCCTGTGGGATGCAAGTGGGAGAATCCACCTCATTGCCTGGAGATCACGCCAC
  CATCTTCAGAAAAGCTGGTCTCAGTGATGCGGTTAAGTGACCTCTCTACAGAAGATGATGACTCAGGTCA
  CTGTAAAATGAACCGTTATGATAAGAAGATTGATAGTCTAATGAATGCGGTTGGTTGTCTGAAGTCTGAG
  GTCAAGATGCAAAAAGGTGAGCGCCAGATGGCCAAAAGGTTCCTGGAGGAACGGAAGGAAGAGCTGGAGG
  AGGTGGCCCACGAACTGGCTGAGACTGAGCACGAGAACACGGTGTTGAGGCACAACATCGAGCGCATGAA
  GGAGGAGAAGGACTTCACCATACTTCAGAAGAAACACCTACAACAGGAGAAGGAGTGCCTCATGTCCAAG
  CTGGTGGAGGCGGAAATGGATGGGGCTGCGGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGA
  AGCTGAAAACGGAGAAACAAATGACCTGCACGGACATCAACACCCTGACAAGGCAGAAGGAACTTCTCCT
  GCAGAAGCTGAGCACATTTGAGGAGACCAACCGCACCCTCCGAGACCTCCTGAGGGAACAGCACTGCAAA
  GAGGATTCTGAAAGACTAATGGAGCAACAAGGAGCACTGCTGAAACGGCTGGCGGAGGCCGACTCAGAGA
  AAGCGCGCCTGCTGTTACTGCTGCAAGACAAGGACAAGGAGGTGGAAGAGCTCCTTCAGGAAATACAATG
  TGAGAAGGCTCAAGCAAAGACAGCCTCTGAGCTTTCTAAATCCATGGAGTCCATGCGTGGGCATTTGCAG
  GCACAGCTTCGGTCCAAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATTAAGAATCTGGAGCGCAGCG
  GGAATCAGCATAAGGCAGAAGTGGAGGCCATCATGGAGCAGCTGAAGGAGTTGAAGCAGAAGGGAGACCG
  AGACAAAGAGAGCTTGAAGAAGGCCATCCGAGCCCAGAAGGAGCGAGCCGAGAAGAGCGAGGAGTATGCT
  GAGCAGCTACACGTGCAACTCGCTGACAAGGATCTTTATGTCGCTGAAGCTTTATCCACTCTGGAATCCT
  GGAGGAGCCGCTACAACCAAGTTGTAAAAGAAAAGGGAGACCTTGAGCTGGAAATTATTGTCCTGAATGA
  CCGGGTAACAGATCTTGTAAACCAACAACAAACCCTGGAGGAGAAGATGCGGGAAGACCGGGATAGCCTG
  GTGGAGAGACTACACCGTCAGACTGCTGAGTATTCCGCATTCAAGCTGGAGAATGAGAGGCTGAAGGCCA
  GCTTTGCTCCAATGGAGGACAAACTCAACCAGGCACACCTCGAGGTCCAGCAGCTGAAGGCCTCAGTGAA
  GAACTATGAGGGGATGATTGACAACTATAAGAGTCAGGTGATGAAGACCAGATTGGAGGCTGATGAAGTA
  GCTGCCCAGCTAGAACGCTGTGACAAAGAGAACAAGATCCTTAAAGATGAGATGAACAAAGAGATTGAGG
  CGGCACGAAGGCAGTTCCAGTCTCAGCTGGCTGACCTGCAGCAGCTCCCTGACATCCTGAAGATCACGGA
  GGCGAAGCTGGCTGAGTGCCAAGACCAACTGCAGGGCTATGAGCGGAAGAACATCGACCTCACAGCCATC
  ATATCAGACCTGCGCAGCCGGGTAAGGGACTGGCAGAAAGGGTCCCACGAACTGACCCGAGCAGGGGCCC
  GCATACCAAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231359 representing NM_153436
  Red=Cloning site Green=Tags(s)
  
  MADQQGPHQNRFPSCGKNGVTSLTQKKVLRAPCGAPSVTVTKSHKRGMKGDTVNVRRSVRVKTKVPWMPP
  GKSSARPVGCKWENPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSE
  VKMQKGERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERMKEEKDFTILQKKHLQQEKECLMSK
  LVEAEMDGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCK
  EDSERLMEQQGALLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQ
  AQLRSKEAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKESLKKAIRAQKERAEKSEEYA
  EQLHVQLADKDLYVAEALSTLESWRSRYNQVVKEKGDLELEIIVLNDRVTDLVNQQQTLEEKMREDRDSL
  VERLHRQTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEV
  AAQLERCDKENKILKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAI
  ISDLRSRVRDWQKGSHELTRAGARIPR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153436
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153436.1, NP_702914.1
• RefSeq Size: 2125 bp
• RefSeq ORF: 1974 bp
• Locus ID: 4957
• UniProt ID: Q5BJF6
• Cytogenetics: 9q34.11
• Gene Summary: The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010]