AOPEP (NM_001193331) Human Tagged ORF Clone

CAT#: RG231317

C9orf3 (tGFP-tagged) - Human chromosome 9 open reading frame 3 (C9orf3), transcript variant 4

Product Images

Circular map for RG231317

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AOPEP
• Synonyms: AP-O; APO; C9orf3; C90RF3; ONPEP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231317 representing NM_001193331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATACAGCTGGACCCTGCCAGAGATGACCTGCCTCTCATGGCCAACACCAGCCACATACTTGTGA
  AGCACTATGTACTGGATTTGGATGTGGATTTTGAAAGTCAAGTCATTGAGGGGACCATAGTGCTTTTCCT
  CGAGGATGGAAACAGATTCAAGAAACAGAATAGCTCTATTGAGGAAGCCTGCCAATCAGAATCAAACAAA
  GCCTGCAAATTTGGGATGCCTGAACCCTGCCATATTCCCGTGACAAATGCAAGGACCTTCTCATCTGAAA
  TGGAATATAATGATTTTGCAATCTGTAGTAAAGGTGAAAAAGATACTTCTGATAAAGATGGTAACCATGA
  CAACCAGGAACATGCTTCTGGGATTTCTAGCTCAAAGTACTGCTGTGACACAGGGAATCATGGGAGTGAG
  GATTTTTTGCTAGTGTTGGACTGCTGTGATTTATCTGTGTTAAAAGTCGAGGAGGTGGATGTTGCTGCTG
  TGCCAGGTCTGGAAAAATTTACAAGGTCTCCTGAGCTCACGGTTGTTTCTGAGGAGTTCAGGAATCAGAT
  TGTACGTGAACTTGTGACTTTGCCTGCAAATCGTTGGAGGGAGCAGTTAGACTATTACGCTCGCTGCAGC
  CAGGCTCCTGGCTGTGGGGAACTCCTCTTTGACACTGACACTTGGAGCTTGCAGATAAGGAAGACAGGGG
  CTCAGACAGCTACTGACTTTCCTCATGCTATCAGGATATGGTACAAAACTAAACCTGAAGGGCGATCGGT
  TACATGGACCTCAGACCAGAGTGGCAGGCCATGTGTTTATACTGTGGGATCTCCCATAAACAACAGGGCC
  CTTTTTCCATGCCAGGAGCCACCCGTTGCCATGTCAACATGGCAGGCTACAGTTCGAGCAGCTGCATCTT
  TTGTTGTTTTAATGAGTGGGGAAAATTCTGCCAAACCAACGCAGCTTTGGGAAGAGTGCTCAAGCTGGTA
  TTACTATGTAACTATGCCAATGCCAGCCTCCACCTTCACAATTGCAGTGGGATGCTGGACAGAAATGAAG
  ATGGAGACATGGTCATCAAATGATTTGGCAACAGAGAGACCCTTCTCACCTTCTGAGGCCAACTTCAGGC
  ATGTTGGTGTTTGCAGTCACATGGAATACCCCTGCCGCTTCCAGAATGCTTCTGCCACCACCCAGGAGAT
  CATTCCTCATCGGGTCTTTGCCCCTGTGTGCCTCACGGGTGCCTGCCAAGAGACCCTTCTGCGGCTGATC
  CCTCCTTGCCTCTCAGCAGCACATTCTGTTCTGGGAGCACACCCGTTCTCTCGGCTGGATGTTCTCATCG
  TCCCTGCCAACTTTCCAAGTCTGGGGATGGCCAGCCCACACATCATGTTCCTCTCTCAGAGCATCTTGAC
  AGGAGGGAACCATCTCTGTGGGACCCGCCTCTGCCATGAAATTGCCCATGCCTGGTTTGGCCTAGCCATC
  GGGGCCCGAGACTGGACGGAGGAGTGGCTGAGTGAAGGCTTCGCCACTCACTTGGAGGATGTGTTTTGGG
  CCACAGCACAGCAGCTGGCCCCCTATGAGGCCCGGGAGCAGCAGGAGCTGAGGGCTTGTCTGCGCTGGCG
  TCGCCTCCAGGACGAGATGCAATGCTCCCCCGAGGAGATGCAGGTGTTAAGGTTTCCACATGTTGGCGGA
  TGCAGTGGAAGCTTCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231317 representing NM_001193331
  Red=Cloning site Green=Tags(s)
  
  MDIQLDPARDDLPLMANTSHILVKHYVLDLDVDFESQVIEGTIVLFLEDGNRFKKQNSSIEEACQSESNK
  ACKFGMPEPCHIPVTNARTFSSEMEYNDFAICSKGEKDTSDKDGNHDNQEHASGISSSKYCCDTGNHGSE
  DFLLVLDCCDLSVLKVEEVDVAAVPGLEKFTRSPELTVVSEEFRNQIVRELVTLPANRWREQLDYYARCS
  QAPGCGELLFDTDTWSLQIRKTGAQTATDFPHAIRIWYKTKPEGRSVTWTSDQSGRPCVYTVGSPINNRA
  LFPCQEPPVAMSTWQATVRAAASFVVLMSGENSAKPTQLWEECSSWYYYVTMPMPASTFTIAVGCWTEMK
  METWSSNDLATERPFSPSEANFRHVGVCSHMEYPCRFQNASATTQEIIPHRVFAPVCLTGACQETLLRLI
  PPCLSAAHSVLGAHPFSRLDVLIVPANFPSLGMASPHIMFLSQSILTGGNHLCGTRLCHEIAHAWFGLAI
  GARDWTEEWLSEGFATHLEDVFWATAQQLAPYEAREQQELRACLRWRRLQDEMQCSPEEMQVLRFPHVGG
  CSGSFS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001193331
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001193331.3
• RefSeq Size: 3851 bp
• RefSeq ORF: 1701 bp
• Locus ID: 84909
• UniProt ID: Q8N6M6
• Cytogenetics: 9q22.32
• Protein Families: Protease
• Gene Summary: This gene encodes a member of the M1 zinc aminopeptidase family. The encoded protein is a zinc-dependent metallopeptidase that catalyzes the removal of an amino acid from the amino terminus of a protein or peptide. This protein may play a role in the generation of angiotensin IV. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]