LIPF (NM_001198828) Human Tagged ORF Clone

CAT#: RG231186

LIPF (tGFP-tagged) - Human lipase, gastric (LIPF), transcript variant 3

Product Images

Circular map for RG231186

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LIPF
• Synonyms: GL; HGL; HLAL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG231186 representing NM_001198828
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTACTGGGGACTACACATGGTTTGTTTGGAAAAT
  TACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCAGATGATTACTTATTGGGGATACCCAAATGA
  AGAATATGAAGTTGTGACTGAAGATGGTTATATTCTTGAAGTCAATAGAATTCCTTATGGGAAGAAAAAT
  TCAGGGAATACAGATGCTGGTTATGATGTGTGGCTGGGCAACAGCAGAGGAAACACCTGGGCCAGAAGAA
  ACTTGTACTATTCACCAGATTCAGTTGAATTCTGGGCTTTCAGCTTTGATGAAATGGCTAAATATGACCT
  TCCAGCCACAATCGACTTCATTGTAAAGAAAACTGGACAGAAGCAGCTACACTATGTTGGCCATTCCCAG
  GGCACCACCATTGGTTTTATTGCCTTTTCCACCAATCCCAGCCTGGCTAAAAGAATCAAAACCTTCTATG
  CTCTAGCTCCTGTTGCCACTGTGAAGTATACAAAAAGCCTTATAAACAAACTTAGATTTGTTCCTCAATC
  CCTCTTCAAGTTTATATTTGGTGACAAAATATTCTACCCACACAACTTCTTTGATCAATTTCTTGCTACT
  GAAGTGTGCTCCCGTGAGATGCTGAATCTCCTTTGCAGCAATGCCTTATTTATAATTTGTGGATTTGACA
  GTAAGAACTTTAACACGAGTCGCTTGGATGTGTATCTATCACATAATCCAGCAGGAACTTCTGTTCAAAA
  CATGTTCCATTGGACCCAGGCTGTTAAGTCTGGGAAATTCCAAGCTTATGACTGGGGAAGCCCAGTTCAG
  AATAGGATGCACTATGATCAGTCCCAACCTCCCTACTACAATGTGACAGCCATGAATGTACCAATTGCAG
  TGTGGAACGGTGGCAAGGACCTGTTGGCTGACCCCCAAGATGTTGGCCTTTTGCTTCCAAAACTCCCCAA
  TCTTATTTACCACAAGGAGATTCCTTTTTACAATCACTTGGACTTTATCTGGGCAATGGATGCCCCTCAA
  GAAGTTTACAATGACATTGTTTCTATGATATCAGAAGATAAAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG231186 representing NM_001198828
  Red=Cloning site Green=Tags(s)
  
  MWLLLTMASLISVLGTTHGLFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKN
  SGNTDAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFIVKKTGQKQLHYVGHSQ
  GTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKLRFVPQSLFKFIFGDKIFYPHNFFDQFLAT
  EVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQ
  NRMHYDQSQPPYYNVTAMNVPIAVWNGGKDLLADPQDVGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQ
  EVYNDIVSMISEDKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198828
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198828.2
• RefSeq Size: 1357 bp
• RefSeq ORF: 1098 bp
• Locus ID: 8513
• UniProt ID: P07098
• Cytogenetics: 10q23.31
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• Gene Summary: This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]