MASTL (NM_001172304) Human Tagged ORF Clone

CAT#: RG230531

MASTL (tGFP-tagged) - Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 3

Product Images

Circular map for RG230531

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MASTL
• Synonyms: GREATWALL; GW; GWL; MAST-L; THC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230531 representing NM_001172304
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCACCGCGGGAAGCAAGAAGGAGCCTGGAGGAGGCGCGGCGACTGAGGAGGGCGTGAATAGGA
  TCGCAGTGCCAAAACCGCCCTCCATTGAGGAATTCAGCATAGTGAAGCCCATTAGCCGGGGCGCCTTCGG
  GAAAGTGTATCTGGGGCAGAAAGGCGGCAAATTGTATGCAGTAAAGGTTGTTAAAAAAGCAGACATGATC
  AACAAAAATATGACTCATCAGGTCCAAGCTGAGAGAGATGCACTGGCACTAAGCAAAAGCCCATTCATTG
  TCCATTTGTATTATTCACTGCAGTCTGCAAACAATGTCTACTTGGTAATGGAATATCTTATTGGGGGAGA
  TGTCAAGTCTCTCCTACATATATATGGTTATTTTGATGAAGAGATGGCTGTGAAATATATTTCTGAAGTA
  GCACTGGCTCTAGACTACCTTCACAGACATGGAATCATCCACAGGGACTTGAAACCGGACAATATGCTTA
  TTTCTAATGAGGGTCATATTAAACTGACGGATTTTGGCCTTTCAAAAGTTACTTTGAATAGAGATATTAA
  TATGATGGATATCCTTACAACACCATCAATGGCAAAACCTAGACAAGATTATTCAAGAACCCCAGGACAA
  GTGTTATCGCTTATCAGCTCGTTGGGATTTAACACACCAATTGCAGAAAAAAATCAAGACCCTGCAAACA
  TCCTTTCAGCCTGTCTGTCTGAAACATCACAGCTTTCTCAAGGACTCGTATGCCCTATGTCTGTAGATCA
  AAAGGACACTACGCCTTATTCTAGCAAATTACTAAAATCATGTCTTGAAACAGTTGCCTCCAACCCAGGA
  ATGCCTGTGAAGTGTCTAACTTCTAATTTACTCCAGTCTAGGAAAAGGCTGGCCACATCCAGTGCCAGTA
  GTCAATCCCACACCTTCATATCCAGTGTGGAATCAGAATGCCACAGCAGTCCCAAATGGGAAAAAGATTG
  CCAGGAAAGTGATGAAGCATTGGGCCCAACAATGATGAGTTGGAATGCAGTTGAAAAGTTATGCGCAAAA
  TCTGCAAATGCCATTGAGACGAAAGGTTTCAATAAAAAGGATCTGGAGTTAGCTCTTTCTCCCATTCATA
  ACAGCAGTGCCCTTCCCACCACTGGACGCTCTTGTGTAAACCTTGCTAAAAAATGCTTCTCTGGGGAAGT
  TTCTTGGGAAGCAGTAGAACTGGATGTAAATAATATAAATATGGACACTGACACAAGTCAGTTAGGTTTC
  CATCAGTCAAATCAGTGGGCTGTGGATTCTGGTGGGATATCTGAAGAGCACCTTGGGAAAAGAAGTTTAA
  AAAGAAATTTTGAGTTGGTTGACTCCAGTCCTTGTAAAAAAATTATACAGAATAAAAAAACTTGTGTAGA
  GTATAAGCATAACGAAATGACAAATTGTTATACAAATCAAAATACAGGCTTAACAGTTGAAGTGCAGGAC
  CTTAAGCTATCAGTGCACAAAAGTCAACAAAATGACTGTGCTAATAAGGAGAACATTGTCAATTCTTTTA
  CTGATAAACAACAAACACCAGAAAAATTACCTATACCAATGATAGCAAAAAACCTTATGTGTGAACTCGA
  TGAAGACTGTGAAAAGAATAGTAAGAGGGACTACTTAAGTTCTAGTTTTCTATGTTCTGATGATGATAGA
  GCTTCTAAAAATATTTCTATGAACTCTGATTCATCTTTTCCTGGAATTTCTATAATGGAAAGTCCATTAG
  AAAGTCAGCCCTTAGATTCAGATAGAAGCATCAAAGAATCCTCTTTTGAAGAATCAAATATTGAAGATCC
  ACTTATTGTAACACCAGATTGCCAAGAAAAGACCTCACCAAAAGGTGTCGAGAACCCTGCTGTACAAGAG
  AGTAACCAAAAAATGTTAGGTCCTCCTTTGGAGGTGCTGAAAACGTTAGCCTCTAAAAGAAATGCTGTTG
  CTTTTCGAAGTTTTAACAGTCATATTAATGCATCCAATAACTCAGAACCATCCAGAATGAACATGACTTC
  TTTAGATGCAATGGATATTTCGTGTGCCTACAGTGGTTCATATCCCATGGCTATAACCCCTACTCAAAAA
  AGAAGATCCTGTATGCCACATCAGACCCCAAATCAGATCAAGTCGGGAACTCCATACCGAACTCCGAAGA
  GTGTGAGAAGAGGGGTGGCCCCCGTTGATGATGGGCGAATTCTAGGAACCCCAGACTACCTTGCACCTGA
  GCTGTTACTAGGCAGGGCCCATGATATCCCTTGGCCAGAAGGTGAAGAAAAGTTATCTGATAATGCTCAA
  AGTGCAGTAGAAATACTTTTAACCATTGATGATACAAAGAGAGCTGGAATGAAAGAGCTAAAACGTCATC
  CTCTCTTCAGTGATGTGGACTGGGAAAATCTGCAGCATCAGACTATGCCTTTCATCCCCCAGCCAGATGA
  TGAAACAGATACCTCCTATTTTGAAGCCAGGAATACTGCTCAGCACCTGACTGTATCTGGATTTAGTCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230531 representing NM_001172304
  Red=Cloning site Green=Tags(s)
  
  MDPTAGSKKEPGGGAATEEGVNRIAVPKPPSIEEFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMI
  NKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEV
  ALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQ
  VLSLISSLGFNTPIAEKNQDPANILSACLSETSQLSQGLVCPMSVDQKDTTPYSSKLLKSCLETVASNPG
  MPVKCLTSNLLQSRKRLATSSASSQSHTFISSVESECHSSPKWEKDCQESDEALGPTMMSWNAVEKLCAK
  SANAIETKGFNKKDLELALSPIHNSSALPTTGRSCVNLAKKCFSGEVSWEAVELDVNNINMDTDTSQLGF
  HQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQNTGLTVEVQD
  LKLSVHKSQQNDCANKENIVNSFTDKQQTPEKLPIPMIAKNLMCELDEDCEKNSKRDYLSSSFLCSDDDR
  ASKNISMNSDSSFPGISIMESPLESQPLDSDRSIKESSFEESNIEDPLIVTPDCQEKTSPKGVENPAVQE
  SNQKMLGPPLEVLKTLASKRNAVAFRSFNSHINASNNSEPSRMNMTSLDAMDISCAYSGSYPMAITPTQK
  RRSCMPHQTPNQIKSGTPYRTPKSVRRGVAPVDDGRILGTPDYLAPELLLGRAHDIPWPEGEEKLSDNAQ
  SAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTMPFIPQPDDETDTSYFEARNTAQHLTVSGFSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172304
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172304.3
• RefSeq Size: 3509 bp
• RefSeq ORF: 2523 bp
• Locus ID: 84930
• UniProt ID: Q96GX5
• Cytogenetics: 10p12.1
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]