KLHDC4 (NM_001184856) Human Tagged ORF Clone

CAT#: RG230169

KLHDC4 (tGFP-tagged) - Human kelch domain containing 4 (KLHDC4), transcript variant 2

Product Images

Circular map for RG230169

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KLHDC4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230169 representing NM_001184856
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGGGCAAGAAGGAGAAGAAGGGCCGCGGCGCGGAGAAGACGGCCGCCAAGATGGAGAAGA
  AGGTGTCTAAGCGCTCGCGGAAGGAGGAGGAAGACCTGGAAGCGCTCATAGCCCATTTCCAGACACTCGA
  TGCCAAGAGGACTCAGACTGTGGAACTTCCGTGCCCCCCACCCTCACCAAGGTTAAATGCCTCCCTCTCG
  GTTCATCCTGAGAAAGATGAGTTAATCCTTTTTGGAGGTGAATATTTCAACGGCCAAAAAACTTTTTTGT
  ATAACGAGCTCTATGTCTACAATACCAGAAAGGACACCTGGACCAAAGTTGACATCCCCAGTCCACCTCC
  GAGGCGCTGTGCTCACCAGGCGGTGGTAGTGCCTCAAGGTGGCGGACAGCTGTGGGTCTTTGGAGGGGAG
  TTTGCCTCTCCCAACGGAGAGCAGTTCTACCACTACAAGGATCTCTGGGTCCTGCATTTGGCCACCAAGA
  CCTGGGAACAAGTCAAGGATTACATCTACTACAACGACGTGTATGCCTTTAATCTGGACACCTTCACATG
  GAGCAAGCTGTCCCCGTCAGGGACGGGGCCCACACCCAGATCAGGCTGCCAGATGTCCGTCACTCCCCAG
  GGCGGCATCGTCGTCTATGGGGGCTACTCGAAACAGAGAGTTAAGAAAGACGTGGACAAGGGCACACGGC
  ACTCAGACATGTTCCTGCTGAAGCCAGAGGACGGAAGAGAAGACAAGTGGGTTTGGACTCGGATGAACCC
  TTCGGGGGTCAAGCCCACCCCACGGTCTGGCTTTTCCGTGGCCATGGCCCCGAATCACCAGACACTGTTC
  TTCGGGGGTGTCTGTGACGAGGAAGAGGAGGAGAGCCTGTCGGGCGAGTTCTTCAACGATCTGTACTTCT
  ACGACGCCACCAGGAACCGTTGGTTTGAGGGACAGCTGAAGGGACCCAAGTCTGAAAAGAAGAAACGCAG
  GCGGGGCAGAAAAGAGGAGCCCGAAGGTGGTAGCAGGCCGGCGTGTGGGGGAGCTGGCACCCAGGGGCCT
  GTGCAGCTGGTCAAGGAGGTGGTGGCCGAGGATGGCACCGTGGTCACCATTAAGCAGGTGCTCACCGCGC
  CAGGCTCGGCGGGGCAGCCCCGGTCTGAGGACGAAGACAGCCTTGAGGAGGCCGGCAGCCCCGCACCTGG
  GCCGTGTCCACGCTCCAACGCCATGCTGGCTGTGAAGCATGGGGTGCTCTACGTCTATGGGGGCATGTTT
  GAGGCCGGCGACCGCCAGGTCACCCTCAGCGACCTGCACTGCCTGGACCTGCACAGGATGGAGGCGTGGA
  AGGCCTTGGTGGAGATGGACCCAGAAACTCAGGAGTGGCTGGAGGAGACGGACTCGGAAGAGGACAGTGA
  GGAGGTTGAGGGCGCCGAGGGTGGGGTCGACGACGAAGACAGCGGAGAGGAGAGCGGTGCGGAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230169 representing NM_001184856
  Red=Cloning site Green=Tags(s)
  
  MGKKGKKEKKGRGAEKTAAKMEKKVSKRSRKEEEDLEALIAHFQTLDAKRTQTVELPCPPPSPRLNASLS
  VHPEKDELILFGGEYFNGQKTFLYNELYVYNTRKDTWTKVDIPSPPPRRCAHQAVVVPQGGGQLWVFGGE
  FASPNGEQFYHYKDLWVLHLATKTWEQVKDYIYYNDVYAFNLDTFTWSKLSPSGTGPTPRSGCQMSVTPQ
  GGIVVYGGYSKQRVKKDVDKGTRHSDMFLLKPEDGREDKWVWTRMNPSGVKPTPRSGFSVAMAPNHQTLF
  FGGVCDEEEEESLSGEFFNDLYFYDATRNRWFEGQLKGPKSEKKKRRRGRKEEPEGGSRPACGGAGTQGP
  VQLVKEVVAEDGTVVTIKQVLTAPGSAGQPRSEDEDSLEEAGSPAPGPCPRSNAMLAVKHGVLYVYGGMF
  EAGDRQVTLSDLHCLDLHRMEAWKALVEMDPETQEWLEETDSEEDSEEVEGAEGGVDDEDSGEESGAED
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001184856
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001184856.2
• RefSeq Size: 1851 bp
• RefSeq ORF: 1470 bp
• Locus ID: 54758
• UniProt ID: Q8TBB5
• Cytogenetics: 16q24.2