DAZ1 (NM_004081) Human Tagged ORF Clone

CAT#: RG229361

DAZ1 (tGFP-tagged) - Human deleted in azoospermia 1 (DAZ1). Note: ORF is codon optimized

Product Images

Circular map for RG229361

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DAZ1
• Synonyms: DAZ; SPGY
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG229361 ORF sequence, codon optimized.
  Due to the complexity of NM_004081, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCAGCTAACCCCGAGACACCTAATAGTACCATTAGCAGGGAGGCGAGCACCCAGTCATCCAGCG
  CAGCAGCATCTCAGGGCTGGGTCCTCCCTGAGGGTAAGATTGTGCCCAACACTGTTTTCGTGGGGGGCAT
  CGATGCTAGAATGGATGAAACCGAGATCGGGTCCTGCTTCGGCAGATATGGATCAGTAAAAGAGGTGAAA
  ATAATCACCAATAGAACAGGCGTTAGTAAAGGTTACGGTTTCGTTAGCTTCGTCAACGACGTGGATGTGC
  AGAAGATCGTAGGCAGCCAAATACACTTTCATGGGAAGAAGTTGAAGCTTGGACCAGCCATTCGCAAACA
  GAAGCTGTGCGCTAGACACGTGCAGCCAAGACCACTTGTGGTAAATCCACCCCCCCCTCCACAGTTTCAG
  AATGTTTGGAGAAACCCCAACACCGAGACATATCTGCAGCCTCAGATCACACCCAACCCAGTCACCCAGC
  ACGTCCAAAGCGCCGCGAATCCAGAAACTCCAAACAGCACCATCTCTAGAGAGGCCTCCACACAGTCCAG
  CTCTGCTGCCGCTAGCCAGGGGTGGGTACTTCCCGAGGGAAAGATCGTGCCAAATACCGTATTCGTGGGG
  GGGATAGATGCCCGCATGGACGAAACTGAGATAGGATCATGCTTCGGCAGGTACGGTTCCGTGAAGGAGG
  TTAAGATTATCACCAATCGGACGGGCGTTAGTAAGGGGTACGGATTCGTGAGTTTCGTGAATGACGTCGA
  TGTGCAAAAAATCGTCGGCTCACAGATTCATTTCCACGGGAAGAAACTCAAGCTGGGGCCAGCAATCAGG
  AAGCAGAAGTTGTGCGCACGGCATGTCCAGCCAAGACCTTTGGTGGTCAACCCACCCCCGCCTCCTCAAT
  TTCAGAACGTGTGGAGAAATCCAAATACAGAAACCTACTTGCAGCCACAGATCACTCCAAATCCCGTTAC
  ACAGCACGTGCAGTCAGCAGCCAACCCCGAGACGCCAAACTCAACAATTTCCAGGGAAGCCAGCACCCAG
  TCCTCTAGCGCTGCCGCTTCACAGGGGTGGGTCCTGCCGGAAGGTAAGATTGTCCCGAATACTGTGTTCG
  TAGGCGGAATCGATGCCCGCATGGATGAGACGGAAATTGGCAGCTGCTTCGGCAGGTACGGCTCCGTTAA
  GGAAGTGAAAATCATCACTAATCGCACTGGTGTGTCAAAGGGGTACGGGTTCGTTAGCTTTGTGAACGAT
  GTCGATGTCCAGAAGATAGTCGGAAGCCAGATCCATTTTCACGGAAAGAAATTGAAGCTTGGCCCTGCAA
  TCCGGAAGCAGAAGCTCTGTGCCAGGCATGTTCAACCACGCCCCCTCGTGGTTAATCCCCCTCCGCCTCC
  CCAGTTCCAGAATGTTTGGCGCAATCCGAACACCGAGACTTACCTGCAGCCCCAAATCACCCCAAATCCT
  GTGACACAGCATGTGCAGGCCTATAGCGCTTACCCCCATTCACCGGGACAGGTGATCACTGGCTGTCAGC
  TGCTGGTTTATAACTACCAGGAATACCCCACATATCCCGACAGCGCTTTTCAGGTGACAACTGGCTATCA
  ACTGCCCGTCTACAACTATCAACCCTTCCCAGCGTATCCCCGGTCACCTTTCCAGGTGACGGCGGGTTAC
  CAGCTTCCTGTGTACAATTACCAGGCATTTCCCGCATACCCTAACTCTCCTTTTCAAGTTGCTACCGGAT
  ATCAGTTCCCAGTCTACAACTATCAGCCCTTCCCTGCCTACCCCTCTTCACCCTTCCAAGTGACCGCAGG
  CTATCAATTGCCAGTGTACAACTACCAGGCTTTCCCCGCATATCCCAATAGTCCTTTCCAAGTTGCTACT
  GGGTACCAATTTCCAGTGTATAATTATCAGGCCTTCCCAGCCTATCCAAATAGCCCTGTGCAGGTAACCA
  CAGGTTATCAGCTGCCAGTCTATAATTACCAGGCCTTTCCCGCGTACCCTTCCTCCCCCTTTCAGGTGAC
  CACTGGTTACCAGCTGCCTGTGTACAACTATCAAGCCTTTCCAGCATATCCCAATTCCGCTGTGCAGGTT
  ACAACCGGTTATCAGTTCCATGTGTATAACTACCAGATGCCCCCCCAATGCCCTGTGGGGGAGCAGCGGA
  GAAACCTCTGGACAGAGGCCTATAAATGGTGGTATCTGGTGTGCCTGATACAGCGCCGAGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG229361 representing NM_004081
  Red=Cloning site Green=Tags(s)
  
  MSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVK
  IITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIRKQKLCARHVQPRPLVVNPPPPPQFQ
  NVWRNPNTETYLQPQITPNPVTQHVQSAANPETPNSTISREASTQSSSAAASQGWVLPEGKIVPNTVFVG
  GIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVNDVDVQKIVGSQIHFHGKKLKLGPAIR
  KQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNPVTQHVQSAANPETPNSTISREASTQ
  SSSAAASQGWVLPEGKIVPNTVFVGGIDARMDETEIGSCFGRYGSVKEVKIITNRTGVSKGYGFVSFVND
  VDVQKIVGSQIHFHGKKLKLGPAIRKQKLCARHVQPRPLVVNPPPPPQFQNVWRNPNTETYLQPQITPNP
  VTQHVQAYSAYPHSPGQVITGCQLLVYNYQEYPTYPDSAFQVTTGYQLPVYNYQPFPAYPRSPFQVTAGY
  QLPVYNYQAFPAYPNSPFQVATGYQFPVYNYQPFPAYPSSPFQVTAGYQLPVYNYQAFPAYPNSPFQVAT
  GYQFPVYNYQAFPAYPNSPVQVTTGYQLPVYNYQAFPAYPSSPFQVTTGYQLPVYNYQAFPAYPNSAVQV
  TTGYQFHVYNYQMPPQCPVGEQRRNLWTEAYKWWYLVCLIQRRD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004081
• ORF Size: 2232 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004081.5, NP_004072.3
• RefSeq Size: 4419 bp
• RefSeq ORF: 2235 bp
• Locus ID: 1617
• UniProt ID: Q9NQZ3
• Cytogenetics: Yq11.223
• Gene Summary: This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains. [provided by RefSeq, Jul 2008]