C20orf19 (KIZ) (NM_001163022) Human Tagged ORF Clone

CAT#: RG228965

PLK1S1 (tGFP-tagged) - Human polo-like kinase 1 substrate 1 (PLK1S1), transcript variant 2

Product Images

Circular map for RG228965

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C20orf19
• Synonyms: C20orf19; HT013; Kizuna; NCRNA00153; PLK1S1; RP69
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228965 representing NM_001163022
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCTCGAATATGAGACTCAAATTAAGAAGATGCTATGCTCAAAAGATAGCCTGGGACTAAAAGAGG
  AACTGACAGATGAAGACAGAGAAAAGGTTGCAGTGCAGGAGGGGATTAACTCAGGAACAGCCATGTCAAG
  AGGATTGTATCAACCAGCAACAATCTTTATGGGCCGCCAAATGTCAGCCATCTTAAGCATGAGAGATTTC
  AGTACAGAGCACAAATCTCCCCAGCCCACAAAGAACTTTTCAATTCCTGACCCACATTCACACCGACAGA
  CAGCCCAGAGCAGTAATGTGACAGACAGCTGTGTAGTACAAACTAGTAATGACACACAGTGCTTAAATAA
  GTCTGACAACATAGATGGAAAGGCATCTCTTCAGATTGGTGAGAAAACGCCAGTCACAGCCAGTGTATTG
  TCTGAGGAGGAACAAACTCATTGCTTGGAGATAGGAAGTAACACACGTCATGGCAAGAGTAATTTATCTG
  AAGGCAAAAAGTCTGCTGAACTCAATTCCCCGTTACGGGAAAGATTAAGTCCAGAGAACAGAACCACTGA
  TTTAAAGTGTGACAGTTCCAGCGGATCAGAGGGAGAAATACTGACACGGGAACATATTGAAGTTGAGGAA
  AAAAGAGCCAGCCCGCCAGTCTCTCCGATACCAGTTTCAGAATACTGTGAATCTGAAAATAAGTGGTCTC
  AAGAGAAGCATTCTCCTTGGGAAGGTGTTTCAGATCATCTTGCTCACAGGGAACCAAAGTCACAAAAGCC
  CTTCAGAAAAATGCAGGAAGAGGAGGAGGAAAGTTGGAGCACCAGCAGTGACCTTACCATTTCAATAAGT
  GAAGATGATCTGATTTTAGAGAGCCCAGAACCACAGCCAAATCCAGGTGGCAAGATGGAGGGAGAAGATG
  GAATAGAGGCCTTAAAATTAATCCATGCTGAGCAAGAAAGAGTTGCCCTATCCACTGAAAAAAATTGTAT
  TTTGCAAACCCTAAGCTCTCCTGATTCAGAAAAGGAATCCTCCACTAACGCACCAACAAGAGAACCTGGA
  CAAACACCAGACTCAGACGTACCGAGGGCACAGGTGGGTCAGCATGTTGCCACCTTGAAAGAACATGATA
  ATTCTGTCAAAGAAGAGGCAACAGCATTATTGAGAAAAGCCCTTACAGAAGAGTGTGGCCGTAGGTCAGC
  TATTCACAGTAGTGAATCATCTTGCAGCTTGCCATCTATTCTGAATGACAATAGTGGAATAAAGGAAGCC
  AAACCTGCTGTATGGCTCAACAGTGTTCCTACAAGGGAACAAGAAGTTTCAAGTGGCTGTGGAGACAAGA
  GCAAGAAAGAAAATGTGGCTGCAGATATCCCAATCACAGAAACAGAAGCCTATCAGTTGCTGAAGAAGGC
  CACCCTTCAGGATAATACAAATCAAACTGAAAACAGGTTTCAAAAGACAGATGCTTCTGTGTCACACTTG
  TCAGGTTTGAATATTGGCAGCGGTGCATTCGAGACAAAGACAGCTAACAAAATTGCTTCGGAAGCTAGTT
  TTTCATCTAGTGAAGGAAGTCCTTTGTCAAGGCATGAAAACAAAAAGAAACCCGTGATCAATTTAAAATC
  TAATGCCCTCTGGGATGAGTCTGATGACAGTAACTCAGAAATTGAGGCTGCTTTACGCCCCAGAAACCAT
  AACACCGATGATTCTGATGATTTTTATGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228965 representing NM_001163022
  Red=Cloning site Green=Tags(s)
  
  MQLEYETQIKKMLCSKDSLGLKEELTDEDREKVAVQEGINSGTAMSRGLYQPATIFMGRQMSAILSMRDF
  STEHKSPQPTKNFSIPDPHSHRQTAQSSNVTDSCVVQTSNDTQCLNKSDNIDGKASLQIGEKTPVTASVL
  SEEEQTHCLEIGSNTRHGKSNLSEGKKSAELNSPLRERLSPENRTTDLKCDSSSGSEGEILTREHIEVEE
  KRASPPVSPIPVSEYCESENKWSQEKHSPWEGVSDHLAHREPKSQKPFRKMQEEEEESWSTSSDLTISIS
  EDDLILESPEPQPNPGGKMEGEDGIEALKLIHAEQERVALSTEKNCILQTLSSPDSEKESSTNAPTREPG
  QTPDSDVPRAQVGQHVATLKEHDNSVKEEATALLRKALTEECGRRSAIHSSESSCSLPSILNDNSGIKEA
  KPAVWLNSVPTREQEVSSGCGDKSKKENVAADIPITETEAYQLLKKATLQDNTNQTENRFQKTDASVSHL
  SGLNIGSGAFETKTANKIASEASFSSSEGSPLSRHENKKKPVINLKSNALWDESDDSNSEIEAALRPRNH
  NTDDSDDFYD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163022
• ORF Size: 1710 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163022.1, NP_001156494.1
• RefSeq Size: 2166 bp
• RefSeq ORF: 1713 bp
• Locus ID: 55857
• UniProt ID: Q2M2Z5
• Cytogenetics: 20p11.23
• Gene Summary: The protein encoded by this gene localizes to centrosomes, strengthening and stabilizing the pericentriolar region prior to spindle formation. The encoded protein usually remains with the mother centrosome after centrosomal duplication. Sevral transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]