SH2D2A (NM_001161443) Human Tagged ORF Clone
CAT#: RG228844
- TrueORF®
SH2D2A (tGFP-tagged) - Human SH2 domain containing 2A (SH2D2A), transcript variant 4
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001161443" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | SH2D2A |
Synonyms | F2771; SCAP; TSAD; VRAP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG228844 representing NM_001161443
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCCCTGGCCCAGATATGTCCCCAAGGGAGTCACGAAGCCCCCATCCCAACCTTCAGCACCT TCCAGATCACAGACATGACCCGCAGGAGCTGCCAGAACCTGGGCTACACTGCGGCATCTCCCCAGGCCCC GGAGGCTGCCTCCAGCACAGGGAATGCTGAGAGGGCAGAGGAGGTGCCTGGAGAAGGAAGCCTGTTCCTG CAGGCCGAGACCCGGGCTTGGTTCCAGAAGACCCAGGCCCACTGGCTCCTGCAGCACGGGGCAGCCCCTG CCTGGTTCCATGGCTTCATCACCCGGAGGGAGGCAGAGAGGCTGCTGGAGCCCAAGCCTCAGGGGTGCTA CTTGGTGCGGTTCAGCGAGAGCGCGGTGACCTTCGTGCTGACTTACAGGAGCCGGACTTGCTGCCGCCAC TTCCTGCTGGCCCAGCTCAGGGACGGGCGCCACGTGGTGCTGGGCGAGGACAGCGCCCACGCGCGGCTGC AGGACCTGCTGCTGCACTACACCGCGCACCCGCTCAGCCCCTACGGGGAGACGCTCACCGAGCCCCTCGC CCGACAGACTCCTGAGCCTGCAGGACTTTCCCTGAGGACCGAAGAATCAAACTTTGGAAGCAAAAGCCAG GACCCAAACCCCCAGTACAGCCCAATCATCAAACAGGGGCAAGCCCCAGTCCCGATGCAGAAAGAGGGGG CCGGGGAGAAGGAGCCCTCCCAGCTGCTCAGGCCCAAGCCTCCCATCCCCGCCAAACCTCAGCTGCCCCC AGAAGTCTACACAATCCCTGTTCCACGACACCGCCCGGCCCCACGCCCCAAGCCCTCCAATCCTATCTAC AATGAGCCTGATGAACCCATAGCTTTCTATGCCATGGGCCGGGGCAGCCCTGGGGAAGCCCCCAGCAACA TCTATGTGGAAGTGGAAGATGAGGGCCTACCCGCCACCCTTGGGCACCCTGTCCTACGGAAGAGCTGGTC CAGGCCTGTCCCAGGAGGCCAGAATACAGGTGGCTCCCAGCTGCATTCTGAGAACTCTGTGATTGGGCAA GGCCCTCCCCTGCCCCACCAGCCCCCACCCGCCTGGAGACACACCCTCCCCCACAATCTTTCTAGACAGG TGCTTCAGGACAGAGGACAGGCATGGCTTCCCCTTGGGCCTCCTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG228844 representing NM_001161443
Red=Cloning site Green=Tags(s) MEFPLAQICPQGSHEAPIPTFSTFQITDMTRRSCQNLGYTAASPQAPEAASSTGNAERAEEVPGEGSLFL QAETRAWFQKTQAHWLLQHGAAPAWFHGFITRREAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRH FLLAQLRDGRHVVLGEDSAHARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQ DPNPQYSPIIKQGQAPVPMQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPSNPIY NEPDEPIAFYAMGRGSPGEAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQNTGGSQLHSENSVIGQ GPPLPHQPPPAWRHTLPHNLSRQVLQDRGQAWLPLGPPQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001161443 |
ORF Size | 1083 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001161443.1, NP_001154915.1 |
RefSeq Size | 1540 bp |
RefSeq ORF | 1086 bp |
Locus ID | 9047 |
Cytogenetics | 1q23.1 |
Protein Pathways | VEGF signaling pathway |
Gene Summary | This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC228844 | SH2D2A (Myc-DDK-tagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 4 |
USD 503.00 |
|
RC228844L3 | Lenti-ORF clone of SH2D2A (Myc-DDK-tagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 4 |
USD 803.00 |
|
RC228844L4 | Lenti-ORF clone of SH2D2A (mGFP-tagged)-Human SH2 domain containing 2A (SH2D2A), transcript variant 4 |
USD 803.00 |
|
SC327479 | SH2D2A (untagged)-Human SH2 domain protein 2A (SH2D2A) transcript variant 4 |
USD 503.00 |
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