LENG4 (MBOAT7) (NM_001146082) Human Tagged ORF Clone

CAT#: RG228833

MBOAT7 (tGFP-tagged) - Human membrane bound O-acyltransferase domain containing 7 (MBOAT7), transcript variant 4

Product Images

Circular map for RG228833

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LENG4
• Synonyms: BB1; hMBOA-7; LENG4; LPIAT; LPLAT; LRC4; MBOA7; MRT57; OACT7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228833 representing NM_001146082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCTGAAGAATGGACGTATCTAGTGGTTCTTCTTATCTCCATCCCCATCGGCTTCCTCTTTAAGA
  AAGCCGGTCCTGGGCTGAAGAGATGGGGAGCAGCCGCTGTGGGCCTGGGGCTCACCCTGTTCACCTGTGG
  CCCCCACACTTTGCATTCTCTGGTCACCATCCTCGGGACCTGGGCCCTCATTCAGGCCCAGCCCTGCTCC
  TGCCACGCCCTGGCTCTGGCCTGGACTTTCTCCTATCTCCTGTTCTTCCGAGCCCTCAGCCTCCTGGGCC
  TGCCCACTCCCACGCCCTTCACCAATGCCGTCCAGCTGCTGCTGACGCTGAAGCTGGTGAGCCTGGCCAG
  TGAAGTCCAGGACCTGCATCTGGCCCAGAGGAAGGAAATGGCCTCAGGCTTCAGCAAGGGGCCCACCCTG
  GGGCTGCTGCCCGACGTGCCCTCCCTGATGGAGACACTCAGCTACAGCTACTGCTACGTGGGAATCATGA
  CAGGCCCGTTCTTCCGCTACCGCACCTACCTGGACTGGCTGGAGCAGCCCTTCCCCGGGGCAGTGCCCAG
  CCTGCGGCCCCTGCTGCGCCGCGCCTGGCCGGCCCCGCTCTTCGGCCTGCTGTTCCTGCTCTCCTCTCAC
  CTCTTCCCGCTGGAGGCCGTGCGCGAGGACGCCTTCTACGCCCGCCCGCTGCCCGCCCGCCTCTTCTACA
  TGATCCCCGTCTTCTTCGCCTTCCGCATGCGCTTCTACGTGGCCTGGATTGCCGCCGAGTGCGGCTGCAT
  TGCCGCCGGCCTTGGGGCCTACCCCGTGGCCGCCAAAGCCCGGGCCGGAGGCGGCCCCACCCTCCAATGC
  CCACCCCCCAGCAGTCCGGAGAAGGCGGCTTCCTTGGAGTATGACTATGAGACCATCCGCAACATCGACT
  GCTACAGCACAGATTTCTGCGTGCGGGTGCGCGATGGCATGCGGTACTGGAACATGACGGTGCAGTGGTG
  GCTGGCGCAGTATATCTACAAGAGCGCACCTGCCCGTTCCTATGTCCTGCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228833 representing NM_001146082
  Red=Cloning site Green=Tags(s)
  
  MSPEEWTYLVVLLISIPIGFLFKKAGPGLKRWGAAAVGLGLTLFTCGPHTLHSLVTILGTWALIQAQPCS
  CHALALAWTFSYLLFFRALSLLGLPTPTPFTNAVQLLLTLKLVSLASEVQDLHLAQRKEMASGFSKGPTL
  GLLPDVPSLMETLSYSYCYVGIMTGPFFRYRTYLDWLEQPFPGAVPSLRPLLRRAWPAPLFGLLFLLSSH
  LFPLEAVREDAFYARPLPARLFYMIPVFFAFRMRFYVAWIAAECGCIAAGLGAYPVAAKARAGGGPTLQC
  PPPSSPEKAASLEYDYETIRNIDCYSTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPARSYVLR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146082
• ORF Size: 1032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146082.1, NP_001139554.1
• RefSeq Size: 2038 bp
• RefSeq ORF: 1035 bp
• Locus ID: 79143
• UniProt ID: Q96N66
• Cytogenetics: 19q13.42
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the membrane-bound O-acyltransferases family of integral membrane proteins that have acyltransferase activity. The encoded protein is a lysophosphatidylinositol acyltransferase that has specificity for arachidonoyl-CoA as an acyl donor. This protein is involved in the reacylation of phospholipids as part of the phospholipid remodeling pathway known as the Land cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]