SLC14A1 (NM_001146037) Human Tagged ORF Clone

CAT#: RG228356

SLC14A1 (tGFP-tagged) - Human solute carrier family 14 (urea transporter), member 1 (Kidd blood group) (SLC14A1), transcript variant 4

Product Images

Circular map for RG228356

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC14A1
• Synonyms: HsT1341; HUT11; JK; Jk(b); RACH1; RACH2; UT-B1; UT1; UTE
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228356 representing NM_001146037
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGACGGTCTTTGATTGGCGGCGCTGGTGACGCCCGTCATGGTCCTGTTTGGAAGGACCCTTTTG
  GAACTAAAGCTGGTGACGCAGCGCGCAGAGGCATCGCCCGGCTAAGCTTGGCCCTGGCAGATGGGTCGCA
  GGAACAGGAGCCAGAGGAAGAGATAGCCATGGAGGACAGCCCCACTATGGTTAGAGTGGACAGCCCCACT
  ATGGTTAGGGGTGAAAACCAGGTTTCGCCATGTCAAGGGAGAAGGTGCTTCCCCAAAGCTCTTGGCTATG
  TCACCGGTGACATGAAAGAACTTGCCAACCAGCTTAAAGACAAACCCGTGGTGCTCCAGTTCATTGACTG
  GATTCTCCGGGGCATATCCCAAGTGGTGTTCGTCAACAACCCCGTCAGTGGAATCCTGATTCTGGTAGGA
  CTTCTTGTTCAGAACCCCTGGTGGGCTCTCACTGGCTGGCTGGGAACAGTGGTCTCCACTCTGATGGCCC
  TCTTGCTCAGCCAGGACAGGTCATTAATAGCATCTGGGCTCTATGGCTACAATGCCACCCTGGTGGGAGT
  ACTCATGGCTGTCTTTTCGGACAAGGGAGACTATTTCTGGTGGCTGTTACTCCCTGTATGTGCTATGTCC
  ATGACTTGCCCAATTTTCTCAAGTGCATTGAATTCCATGCTCAGCAAATGGGACCTCCCCGTCTTCACCC
  TCCCTTTCAACATGGCGTTGTCAATGTACCTTTCAGCCACAGGACATTACAATCCATTCTTTCCAGCCAA
  ACTGGTCATACCTATAACTACAGCTCCAAATATCTCCTGGTCTGACCTCAGTGCCCTGGAGTTGTTGAAA
  TCTATACCAGTGGGAGTTGGTCAGATCTATGGCTGTGATAATCCATGGACAGGGGGCATTTTCCTGGGAG
  CCATCCTACTCTCCTCCCCACTCATGTGCCTGCATGCTGCCATAGGATCATTGCTGGGCATAGCAGCGGG
  ACTCAGTCTTTCAGCCCCATTTGAGGACATCTACTTTGGACTCTGGGGTTTCAACAGCTCTCTGGCCTGC
  ATTGCAATGGGAGGAATGTTCATGGCGCTCACCTGGCAAACCCACCTCCTGGCTCTTGGCTGTGCCCTGT
  TCACGGCCTATCTTGGAGTCGGCATGGCAAACTTTATGGCTGAGGTTGGATTGCCAGCTTGTACCTGGCC
  CTTCTGTTTGGCCACGCTATTGTTCCTCATCATGACCACAAAAAATTCCAACATCTACAAGATGCCCCTC
  AGTAAAGTTACTTATCCTGAAGAAAACCGCATCTTCTACCTGCAAGCCAAGAAAAGAATGGTGGAAAGCC
  CTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228356 representing NM_001146037
  Red=Cloning site Green=Tags(s)
  
  MNGRSLIGGAGDARHGPVWKDPFGTKAGDAARRGIARLSLALADGSQEQEPEEEIAMEDSPTMVRVDSPT
  MVRGENQVSPCQGRRCFPKALGYVTGDMKELANQLKDKPVVLQFIDWILRGISQVVFVNNPVSGILILVG
  LLVQNPWWALTGWLGTVVSTLMALLLSQDRSLIASGLYGYNATLVGVLMAVFSDKGDYFWWLLLPVCAMS
  MTCPIFSSALNSMLSKWDLPVFTLPFNMALSMYLSATGHYNPFFPAKLVIPITTAPNISWSDLSALELLK
  SIPVGVGQIYGCDNPWTGGIFLGAILLSSPLMCLHAAIGSLLGIAAGLSLSAPFEDIYFGLWGFNSSLAC
  IAMGGMFMALTWQTHLLALGCALFTAYLGVGMANFMAEVGLPACTWPFCLATLLFLIMTTKNSNIYKMPL
  SKVTYPEENRIFYLQAKKRMVESPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146037
• ORF Size: 1335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146037.1, NP_001139509.1
• RefSeq Size: 4226 bp
• RefSeq ORF: 1338 bp
• Locus ID: 6563
• UniProt ID: Q13336
• Cytogenetics: 18q12.3
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a membrane transporter that mediates urea transport in erythrocytes. This gene forms the basis for the Kidd blood group system. [provided by RefSeq, Mar 2009]