DMTF1 (NM_001142326) Human Tagged ORF Clone

CAT#: RG227942

DMTF1 (tGFP-tagged) - Human cyclin D binding myb-like transcription factor 1 (DMTF1), transcript variant 3

Product Images

Circular map for RG227942

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DMTF1
• Synonyms: DMP1; DMTF; hDMP1; MRUL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227942 representing NM_001142326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACAGTGGAAGAGGATTCTGACACAGTAACAGTAGAAACTGTGAACTCTGTGACTTTGACTCAGG
  ACACAGAAGGGAATCTCATTCTTCACTGCCCTCAGAATGAAGCGGATGAAATAGACTCAGAAGATAGTAT
  TGAACCTCCACATAAAAGGCTTTGTTTGTCCTCTGAGGATGATCAGAGTATTGATGATTCTACTCCTTGC
  ATATCAGTTGTTGCACTTCCACTTTCAGAAAATGATCAGAGCTTTGAAGTGACCATGACTGCAACCACAG
  AAGTAGCAGATGATGAGGTTACTGAGGGGACTGTGACACAGATACAGATTTTGCAGAATGAGCAACTAGA
  TGAAATATCTCCCTTGGGTAACGAGGAAGTTTCAGCAGTTAGCCAAGCATGGTTTACAACTAAAGAAGAT
  AAGGATTCTCTGACTAATAAAGGACATAAATGGAAGCAGGGGATGTGGTCCAAGGAAGAAATTGATATTT
  TGATGAACAATATTGAACGCTATCTTAAGGCACGCGGAATAAAAGATGCTACAGAAATCATCTTTGAGAT
  GTCAAAAGACGAAAGAAAAGATTTCTACAGGACTATAGCATGGGGTCTGAACCGGCCTTTGTTTGCAGTT
  TATAGAAGAGTGCTTCGCATGTATGATGACAGAAACCATGTGGGAAAATATACACCTGAAGAAATTGAGA
  AGCTCAAGGAGCTCCGGATAAAGCATGGCAATGACTGGGCAACAATAGGGGCGGCGCTAGGAAGAAGTGC
  ATCTTCTGTCAAAGATCGGTGCCGACTGATGAAGGATACTTGCAACACAGGGAAGTGGACAGAAGAAGAA
  GAAAAGAGACTTGCAGAAGTGGTTCATGAGTTGACAAGCACTGAGCCAGGTGACATAGTCACACAGGGTG
  TGTCTTGGGCAGCTGTGGCTGAACGAGTCGGTACCCGCTCAGAAAAGCAATGTCGTTCTAAATGGCTCAA
  CTACCTGAATTGGAAACAGAGTGGGGGTACTGAATGGACCAAGGAAGATGAAATCAATCTCATCCTCAGG
  ATAGCAGAACTTGATGTAGCTGATGAAAATGACATTAACTGGGATCTGTTAGCTGAGGGATGGAGTAGTG
  TCCGTTCACCACAATGGCTACGAAGTAAATGGTGGACCATCAAAAGGCAAATTGCAAACCATAAGGATGT
  TTCGTTCCCTGTCTTAATAAAAGGTCTTAAACAGTTACATGAGAACCAAAAAAACAACCCAACGCTTTTG
  GAGAATAAATCAGGATCTGGAGTTCCAAACAGTAATACCAATTCCAGTGTGCAGCATGTTCAGATAAGAG
  TTGCCCGCTTGGAAGATAATACAGCCATCTCTTCTAGCCCCATGGCAGCATTGCAGATTCCAGTCCAGAT
  CACCCATGTTTCTTCAGCAGACTCTCCTGCTACCGTTGACTCAGAAACAATAACACTAAACAGTGGAACA
  CTACAGACATTTGAGATTCTTCCCTCTTTCCATCTACAGCCCACTGGCACTCCAGGCACCTACCTACTTC
  AAACAAGCTCAAGCCAAGGCCTTCCCCTAACTCTGACTGCTAGTCCCACAGTAACCCTGACAGCTGCTGC
  TCCTGCTTCTCCTGAACAGATTATTGTTCATGCTTTATCCCCAGAACATTTGTTGAACACAAGTGATAAT
  GTTACAGTGCAGTGTCACACACCAAGAGTCATCATTCAGACTGTTGCCACAGAGGACATCACTTCTTCCA
  TATCCCAAGCAGAACTGACAGTCGATAGTGATATTCAGTCATCTGATTTTCCTGAGCCTCCAGACGCCCT
  AGAAGCAGACACTTTCCCAGATGAAGTTCATCACCCTAAGATGACTGTGGAGCCATCATTTAATGATGCT
  CATGTATCCAAATTCAGTGACCAAAATAGCACAGAACTGATGAATAGTGTTATGGTCAGAACAGAAGAAG
  AAATCTCTGACACCGACCTTAAACAAGAGGAATCACCCTCTGATTTAGCCAGTGCTTATGTTACTGAGGG
  TTTAGAGTCTCCCACTATAGAAGAACAAGTTGATCAAACAATTGATGATGAAACAATACTTATCGTTCCT
  TCACCACATGGCTTTATCCAGGCATCTGATGTTATAGATACTGAATCTGTCTTGCCTTTGACAACACTAA
  CAGATCCCATACTCCAACATCATCAGGAAGAATCAAATATCATTGGATCATCCTTGGGCAGTCCTGTTTC
  AGAAGATTCAAAGGATGTCGAAGATTTGGTAAACTGTCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227942 representing NM_001142326
  Red=Cloning site Green=Tags(s)
  
  MSTVEEDSDTVTVETVNSVTLTQDTEGNLILHCPQNEADEIDSEDSIEPPHKRLCLSSEDDQSIDDSTPC
  ISVVALPLSENDQSFEVTMTATTEVADDEVTEGTVTQIQILQNEQLDEISPLGNEEVSAVSQAWFTTKED
  KDSLTNKGHKWKQGMWSKEEIDILMNNIERYLKARGIKDATEIIFEMSKDERKDFYRTIAWGLNRPLFAV
  YRRVLRMYDDRNHVGKYTPEEIEKLKELRIKHGNDWATIGAALGRSASSVKDRCRLMKDTCNTGKWTEEE
  EKRLAEVVHELTSTEPGDIVTQGVSWAAVAERVGTRSEKQCRSKWLNYLNWKQSGGTEWTKEDEINLILR
  IAELDVADENDINWDLLAEGWSSVRSPQWLRSKWWTIKRQIANHKDVSFPVLIKGLKQLHENQKNNPTLL
  ENKSGSGVPNSNTNSSVQHVQIRVARLEDNTAISSSPMAALQIPVQITHVSSADSPATVDSETITLNSGT
  LQTFEILPSFHLQPTGTPGTYLLQTSSSQGLPLTLTASPTVTLTAAAPASPEQIIVHALSPEHLLNTSDN
  VTVQCHTPRVIIQTVATEDITSSISQAELTVDSDIQSSDFPEPPDALEADTFPDEVHHPKMTVEPSFNDA
  HVSKFSDQNSTELMNSVMVRTEEEISDTDLKQEESPSDLASAYVTEGLESPTIEEQVDQTIDDETILIVP
  SPHGFIQASDVIDTESVLPLTTLTDPILQHHQEESNIIGSSLGSPVSEDSKDVEDLVNCH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142326
• ORF Size: 2016 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142326.1, NP_001135798.1
• RefSeq Size: 3684 bp
• RefSeq ORF: 2019 bp
• Locus ID: 9988
• UniProt ID: Q9Y222
• Cytogenetics: 7q21.12
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a transcription factor that contains a cyclin D-binding domain, three central Myb-like repeats, and two flanking acidic transactivation domains at the N- and C-termini. The encoded protein is induced by the oncogenic Ras signaling pathway and functions as a tumor suppressor by activating the transcription of ARF and thus the ARF-p53 pathway to arrest cell growth or induce apoptosis. It also activates the transcription of aminopeptidase N and may play a role in hematopoietic cell differentiation. The transcriptional activity of this protein is regulated by binding of D-cyclins. This gene is hemizygously deleted in approximately 40% of human non-small-cell lung cancer and is a potential prognostic and gene-therapy target for non-small-cell lung cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]