C8orf45 (MCMDC2) (NM_001136160) Human Tagged ORF Clone

CAT#: RG227765

MCMDC2 (tGFP-tagged) - Human chromosome 8 open reading frame 45 (C8orf45), transcript variant 2

Product Images

Circular map for RG227765

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C8orf45
• Synonyms: C8orf45
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG227765 representing NM_001136160
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAATCTAAAAATGAAAGAGGCGGCCCTCATCTATCTTGACAGAAGTGGAGGCCTCCAAAAGTTTA
  TAGATGATTGCAAGTACTACAATGATTCAAAACAAAGCTATGCTGTCTATCGATTCAAAATTTTAATAAA
  TCCCTCTGATGTTGTTGAATTAGATGCTGAGCTTGGAAATCACATTTTACACCAACCTTTAAAAGCTGCT
  GAAGTCTTTCAATCAGTCTGTTTTATTGCTGTTAAGACTCTCTCATTAATTGGACAATTGCAGACTGAAA
  CGCAAATTAATATAGTGCTGAAATTAACACATTTACCTCCCCTGCCAAGTTATGGTCTTGATCTTTGTGA
  GTTTCCACTTGATTATACATCTCAGAGATTTTATATGATGCAAGGAATTGTGATTGCAATGACAACTATA
  ACCAAGTATACACAAGGGGCAAGATTTCTTTGTTCAGATGAAGCATGCCCTCTTTCAAAAGGATTTCAGT
  ATATAAGAGTGCATGTGCCTGGTGCTACAGAATCTGCAACGATAAGAAATGACTTTTTGTGTAATCTATG
  TGCATCTTCACTTCAAGAAGACAGAAAATTTAGAGTACTTGGTGATAAACAAATAGTTGAAATAATTGCC
  ACAAAGGCACTTCGTGCTTTTCAAGGATATTCTAACAACCAGCCATTTAGGTTTCAATCACTTACAATTT
  TCCTAAGAGATGAATCAGTGAATAAAATGAATATAGGAAATGAATATAAAATTATTGGAATTCCAACCTG
  TGTAAAAACCTCACAAACTGCTGTCTGTATAGAAGCAAATAGCATAACTTTTTGTAATTCAAAAGTTCCT
  TCAGGAATTAGTGACAACTTCAGGTGTCTCCTCTCCTTAACTTCCAGCTCATGCTGGAAGTTTACAGCAA
  TACTTGCCAATATCTTTGCATCACAAATTACCCCTCCTGGGACTTACAATTTGCTCAAGCTCTGTTTGTT
  GATGAGTCTAGTACAGACAACTGACCGTAACAAGGAACTGGAAGATTGCCTGGATATTTTAATTATAACA
  AGTGATACTCTACTCATAGACAGGCTTCTGAATTTTAGCATAAACCTTGTCCCCCGTGGTATACGTCATC
  TAGTCTCTACTGAAATTTTTCCCACTCTATCCAGGAATAAGTATGGAACTGGAGCAGTTAGCATTCAGGC
  TGGCAGTGCTTTGCTAGCTAAAGGTGGTATCTGCTTTATAGGAGACTTGGCTTCACACAAAAAAGATAAA
  CTTGAACAGCTTCAAACAGTTCTGGAGAGCAGAAGCATCACAGTGTACATCCCAGGAAAGAAGTTTGGGG
  AGGATATTGATCAACAGATGACTTTTCCAGTTCAGTGCAGTTTTTGGTCTTTTGTTGATGTGGATTCATC
  TTCAAGGAGAAATGCACAGAAAATCAACACTCTAATTGGTCAGATGGATTGCAGTTTGATTCCAGCTAAC
  CTTGTGGAGGCATTTGGTTTATTGATTAACTGCAACGAGTCATCTCCCTGCCACCCATTTCTTCCTACTG
  TGCAACACACTTTGAACAAAGCCATTAATCCTGAAGGGCTGTTTTATGCGGCTTCTAGACAGTTCACAAC
  TGAAGATTTTGAAAAGCTATTGGCTTTTGCAAAGAATTTGAATGTAGAATTCAGCTTGGAAGCAGAAAGA
  ATGACCCATGGCTATTATCTAGCAAGTCGCAGAATCAGAACAGGCTCTGTATGTGGATCAAAGCTGTCAG
  CATCTGCATTAAAATATCTCGTTTTCCTATCTGAAGCCCATGCACGACTGAACTTAAGGAACAAAGTGCT
  TAAAGAAGATGTGCTGATTGCAGCCTTACTATTTGAAACATCCCTCACATTGAAATATGGTAATGAATTA
  AATTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG227765 representing NM_001136160
  Red=Cloning site Green=Tags(s)
  
  MSNLKMKEAALIYLDRSGGLQKFIDDCKYYNDSKQSYAVYRFKILINPSDVVELDAELGNHILHQPLKAA
  EVFQSVCFIAVKTLSLIGQLQTETQINIVLKLTHLPPLPSYGLDLCEFPLDYTSQRFYMMQGIVIAMTTI
  TKYTQGARFLCSDEACPLSKGFQYIRVHVPGATESATIRNDFLCNLCASSLQEDRKFRVLGDKQIVEIIA
  TKALRAFQGYSNNQPFRFQSLTIFLRDESVNKMNIGNEYKIIGIPTCVKTSQTAVCIEANSITFCNSKVP
  SGISDNFRCLLSLTSSSCWKFTAILANIFASQITPPGTYNLLKLCLLMSLVQTTDRNKELEDCLDILIIT
  SDTLLIDRLLNFSINLVPRGIRHLVSTEIFPTLSRNKYGTGAVSIQAGSALLAKGGICFIGDLASHKKDK
  LEQLQTVLESRSITVYIPGKKFGEDIDQQMTFPVQCSFWSFVDVDSSSRRNAQKINTLIGQMDCSLIPAN
  LVEAFGLLINCNESSPCHPFLPTVQHTLNKAINPEGLFYAASRQFTTEDFEKLLAFAKNLNVEFSLEAER
  MTHGYYLASRRIRTGSVCGSKLSASALKYLVFLSEAHARLNLRNKVLKEDVLIAALLFETSLTLKYGNEL
  NY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136160
• ORF Size: 1896 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136160.1, NP_001129632.1
• RefSeq Size: 2048 bp
• RefSeq ORF: 1899 bp
• Locus ID: 157777
• Cytogenetics: 8q13.1
• Gene Summary: Plays an important role in meiotic recombination and associated DNA double-strand break repair.[UniProtKB/Swiss-Prot Function]