DIS3 (NM_001128226) Human Tagged ORF Clone

CAT#: RG226264

DIS3 (tGFP-tagged) - Human DIS3 mitotic control homolog (S. cerevisiae) (DIS3), transcript variant 2

Product Images

Circular map for RG226264

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DIS3
• Synonyms: 2810028N01Rik; dis3p; EXOSC11; KIAA1008; RRP44
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226264 representing NM_001128226
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAAGTCCAAGACGTTCTTAAAAAAGACCCGGGCGGGCGGCGTGATGAAGATCGTGCGCGAGCACT
  ACCTGCGAGACGACATCGGCTGCGGTGCGCCCGGGTGCGCAGCGTGTGGAGGGGCGCACGAGGGGCCGGC
  CCTGGAGCCGCAGCCCCAGGACCCGGCGAGCAGCGTCTGCCCGCAACCGCACTACTTGCTGCCCGACACT
  AATGTGTTACTGCACCAGATTGTAAGTGCCTGGAGGCCGGGGACCTGGGCTTCTGTGGCCTCCAGCCTGC
  GACTCCCAGGCAGCTTAGAAACCTATGTAGAACAAGAACAGGGAGAAAATGCTAATGACAGGAATGATAG
  AGCGATTCGAGTAGCAGCAAAATGGTACAATGAACATTTGAAAAAAATGTCAGCAGACAACCAGCTGCAA
  GTTATCTTCATAACAAATGACAGGAGAAACAAAGAGAAAGCCATAGAAGAAGGAATACCAGCTTTCACTT
  GTGAAGAATATGTAAAGAGCCTAACTGCTAACCCCGAACTCATAGATCGTCTTGCTTGTTTGTCTGAAGA
  AGGGAATGAAATAGAAAGTGGAAAAATAATATTTTCAGAGCATCTTCCCTTAAGTAAGCTACAGCAAGGC
  ATAAAATCTGGTACATACCTTCAAGGAACATTTAGAGCTAGCAGGGAAAATTACTTGGAAGCTACAGTAT
  GGATTCATGGCGACAATGAAGAAAATAAAGAGATAATCTTACAGGGACTTAAACATTTAAACAGAGCTGT
  TCACGAAGATATTGTGGCTGTGGAGCTTCTCCCCAAGAGTCAGTGGGTAGCACCATCTTCTGTGGTTTTA
  CATGATGAAGGTCAAAATGAAGAAGATGTGGAGAAAGAAGAAGAGACAGAACGAATGCTTAAGACTGCTG
  TAAGCGAGAAAATGTTGAAGCCTACAGGTAGAGTTGTAGGAATAATAAAAAGGAATTGGAGACCATATTG
  TGGCATGCTTTCCAAGTCTGACATTAAGGAGTCAAGAAGACATCTCTTTACACCTGCTGATAAGAGAATC
  CCTCGAATTCGCATAGAAACCAGACAGGCTTCCACATTAGAAGGACGGAGAATTATTGTTGCTATTGATG
  GTTGGCCCAGAAATTCCAGATATCCAAATGGACACTTTGTGAGAAATTTAGGTGATGTTGGAGAGAAAGA
  GACTGAAACAGAAGTTTTGTTACTTGAACACGATGTTCCCCATCAGCCTTTTTCACAGGCTGTTCTTAGT
  TTTCTGCCAAAGATGCCCTGGAGCATTACTGAAAAGGACATGAAAAACCGAGAAGACCTGAGGCATCTGT
  GTATTTGTAGTGTAGACCCACCAGGATGTACTGATATAGACGATGCTCTACATTGTCGAGAACTCGAAAA
  TGGAAATTTGGAGGTTGGTGTTCATATTGCTGATGTGAGCCATTTTATTAGGCCAGGAAATGCCTTGGAT
  CAAGAATCAGCCAGAAGAGGAACAACTGTGTATCTTTGTGAAAAGAGGATTGACATGGTTCCAGAGTTGC
  TTAGCTCTAACTTGTGTTCCTTAAAATGTGACGTGGACAGGCTGGCATTTTCATGTATTTGGGAAATGAA
  TCACAATGCTGAAATCTTAAAAACGAAGTTTACCAAAAGTGTTATTAATTCAAAGGCATCTCTGACGTAT
  GCTGAAGCTCAGTTGAGAATTGATTCAGCAAACATGAATGATGATATTACCACTAGTCTCCGTGGACTGA
  ATAAACTAGCCAAAATTCTGAAGAAAAGAAGGATTGAAAAAGGGGCTTTGACTCTATCCTCTCCTGAAGT
  TCGATTCCACATGGACAGTGAAACTCACGATCCTATAGATCTGCAGACCAAGGAACTTAGGGAAACAAAT
  TCCATGGTTGAAGAATTTATGTTACTTGCCAATATTTCTGTTGCAAAAAAAATTCATGAGGAATTTTCTG
  AACATGCTCTGCTTCGAAAACATCCTGCTCCACCTCCATCAAATTATGAAATTCTTGTTAAGGCAGCCAG
  GTCAAGGAATTTGGAAATTAAGACTGATACAGCCAAGTCTTTGGCTGAGTCTTTGGATCAGGCCGAATCT
  CCTACTTTTCCATATCTAAACACTCTGTTGAGAATATTAGCCACTCGCTGTATGATGCAAGCTGTGTACT
  TCTGTTCTGGAATGGATAATGATTTTCATCACTATGGCTTAGCGTCTCCAATATACACACATTTTACTTC
  ACCCATTAGAAGATACGCAGATGTCATTGTTCATCGGCTTTTGGCTGTGGCTATTGGGGCTGACTGTACT
  TATCCAGAGTTGACAGACAAACACAAGCTTGCAGATATATGTAAAAATCTAAATTTCCGGCACAAAATGG
  CTCAATATGCCCAACGTGCATCAGTGGCTTTTCATACCCAGTTATTCTTCAAAAGCAAAGGAATAGTAAG
  TGAAGAAGCCTATATTTTATTTGTAAGAAAGAATGCCATTGTGGTATTAATTCCAAAGTATGGTTTAGAA
  GGGACAGTCTTTTTTGAAGAAAAGGACAAACCAAACCCACAGCTTATTTATGATGATGAGATACCCTCAC
  TTAAAATAGAAGATACAGTGTTCCATGTATTTGATAAAGTTAAAGTGAAAATCATGTTAGACTCATCTAA
  TCTTCAACATCAGAAGATCCGAATGTCCCTGGTAGAACCACAGATACCAGGAATAAGCATTCCTACTGAT
  ACTTCAAACATGGACCTTAATGGACCAAAGAAAAAGAAGATGAAGCTTGGAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226264 representing NM_001128226
  Red=Cloning site Green=Tags(s)
  
  MLKSKTFLKKTRAGGVMKIVREHYLRDDIGCGAPGCAACGGAHEGPALEPQPQDPASSVCPQPHYLLPDT
  NVLLHQIVSAWRPGTWASVASSLRLPGSLETYVEQEQGENANDRNDRAIRVAAKWYNEHLKKMSADNQLQ
  VIFITNDRRNKEKAIEEGIPAFTCEEYVKSLTANPELIDRLACLSEEGNEIESGKIIFSEHLPLSKLQQG
  IKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGLKHLNRAVHEDIVAVELLPKSQWVAPSSVVL
  HDEGQNEEDVEKEEETERMLKTAVSEKMLKPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRI
  PRIRIETRQASTLEGRRIIVAIDGWPRNSRYPNGHFVRNLGDVGEKETETEVLLLEHDVPHQPFSQAVLS
  FLPKMPWSITEKDMKNREDLRHLCICSVDPPGCTDIDDALHCRELENGNLEVGVHIADVSHFIRPGNALD
  QESARRGTTVYLCEKRIDMVPELLSSNLCSLKCDVDRLAFSCIWEMNHNAEILKTKFTKSVINSKASLTY
  AEAQLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETN
  SMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAES
  PTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCT
  YPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLE
  GTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTD
  TSNMDLNGPKKKKMKLGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128226
• ORF Size: 2784 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128226.3
• RefSeq Size: 7577 bp
• RefSeq ORF: 2787 bp
• Locus ID: 22894
• UniProt ID: Q9Y2L1
• Cytogenetics: 13q21.33
• Protein Pathways: RNA degradation
• Gene Summary: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.[UniProtKB/Swiss-Prot Function]