SEZ6L2 (NM_001114099) Human Tagged ORF Clone

CAT#: RG226207

SEZ6L2 (tGFP-tagged) - Human seizure related 6 homolog (mouse)-like 2 (SEZ6L2), transcript variant 3

Product Images

Circular map for RG226207

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SEZ6L2
• Synonyms: BSRPA; PSK-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226207 representing NM_001114099
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACTCCCAGGGCCCAGCACCCGCCGCCTCCCCAGCTGCTGTTCCTAATTCTGCTGAGCTGTCCCT
  GGATCCAGGGTCTGCCCCTGAAGGAGGAGGAGATATTGCCAGAGCCTGGAAGTGAGACCCCCACGGTGGC
  CTCTGAGGCCCTGGCTGAACTGCTTCATGGGGCCCTGCTGAGGAGGGGCCCAGAGATGGGCTACCTGCCA
  GGGCCTCCCCTTGGGCCTGAGGGAGGAGAGGAGGAGACGACGACCACCATCATCACCACGACAACTGTTA
  CCACTACGGTGACCAGCCCAGTTCTGTGTAATAACAACATCTCCGAGGGCGAAGGGTATGTGGAGTCTCC
  AGATCTGGGGAGCCCCGTCAGCCGCACCCTGGGGCTCCTGGACTGCACTTACAGCATCCATGTCTACCCT
  GGCTACGGCATTGAGATCCAGGTGCAGACGCTGAACCTGTCACAGGAAGAGGAGCTCCTGGTGCTGGCTG
  GTGGGGGATCCCCAGGCCTGGCCCCCCGACTCCTGGCCAACTCATCCATGCTTGGAGAAGGACAAGTCCT
  TCGGAGCCCAACCAACCGGCTGCTTCTGCACTTCCAGAGCCCACGGGTCCCAAGGGGCGGTGGCTTCAGG
  ATCCACTATCAGGCCTACCTCCTGAGCTGTGGCTTCCCTCCCCGGCCGGCCCATGGGGACGTGAGTGTGA
  CGGACCTGCACCCTGGGGGCACTGCCACCTTTCACTGTGATTCGGGCTACCAGCTGCAGGGAGAGGAGAC
  CCTCATCTGCCTCAATGGCACCCGGCCATCCTGGAACGGTGAAACCCCCAGCTGCATGGCATCCTGTGGT
  GGCACCATCCACAATGCCACCCTGGGCCGCATCGTGTCCCCAGAGCCTGGGGGAGCCGTAGGGCCCAACC
  TCACCTGCCGTTGGGTCATTGAAGCAGCTGAGGGGCGCCGGCTGCACCTGCACTTTGAAAGGGTCTCGCT
  GGATGAGGACAATGACCGGCTGATGGTGCGCTCAGGGGGCAGCCCCCTATCCCCCGTGATCTATGATTCG
  GACATGGACGATGTCCCCGAGCGGGGTCTCATCAGTGACGCCCAGTCCCTCTACGTGGAGCTGCTGTCAG
  AGACACCTGCCAATCCCCTGCTGTTAAGCCTTCGATTTGAAGCCTTTGAGGAGGATCGCTGCTTCGCCCC
  CTTCCTGGCACATGGAAATGTCACTACCACGGACCCTGAGTATCGCCCAGGGGCACTGGCAACCTTCTCG
  TGCCTCCCAGGATATGCCCTGGAGCCCCCTGGGCCCCCCAATGCCATCGAATGTGTGGATCCCACAGAAC
  CCCACTGGAACGACACAGAGCCGGCCTGCAAAGCCATGTGTGGAGGGGAGCTGTCGGAACCAGCTGGCGT
  GGTCCTCTCTCCCGACTGGCCCCAGAGCTATAGCCCGGGCCAAGACTGCGTGTGGGGCGTGCACGTCCAG
  GAAGAGAAGCGCATCTTGCTCCAAGTTGAGATATTGAATGTGCGGGAAGGGGACATGCTGACGCTGTTCG
  ACGGGGACGGTCCCAGCGCCCGAGTCTTGGCCCAGCTGCGGGGACCTCAGCCGCGCCGCCGCCTTCTCTC
  CTCTGGGCCCGACCTCACACTGCAGTTTCAGGCACCGCCCGGGCCCCCAAATCCAGGCCTGGGCCAGGGC
  TTCGTATTGCACTTCAAAGAGGTCCCGAGGAACGACACGTGCCCCGAGCTGCCACCTCCGGAGTGGGGCT
  GGAGAACGGCATCCCACGGGGACCTGATCCGGGGCACGGTGCTCACCTACCAGTGCGAGCCTGGCTACGA
  GCTGCTAGGCTCCGACATTCTCACTTGCCAGTGGGACCTGTCTTGGAGCGCCGCGCCGCCCGCCTGCCAA
  AAGATCATGACTTGTGCTGACCCTGGCGAGATTGCCAACGGGCACCGCACCGCCTCGGACGCCGGCTTCC
  CCGTTGGCTCCCACGTCCAGTACCGCTGCCTGCCAGGGTACAGCCTCGAGGGGGCAGCCATGCTCACCTG
  CTACAGCCGGGACACAGGCACACCCAAGTGGAGCGATAGGGTCCCCAAATGCGCCTTGAAGTACGAGCCG
  TGCCTGAACCCGGGGGTTCCCGAGAATGGCTACCAGACGCTGTACAAGCACCACTACCAGGCGGGCGAGT
  CTCTGCGCTTCTTCTGCTATGAGGGCTTTGAGCTTATCGGCGAGGTCACCATCACCTGTGTGCCCGGCCA
  CCCCTCCCAGTGGACCAGCCAGCCCCCACTCTGCAAAGTGACCCAGACCACAGATCCATCACGGCAGCTG
  GAAGGGGGGAACCTGGCCCTGGCCATCCTGCTGCCTCTAGGCTTGGTCATTGTCCTCGGCAGTGGCGTTT
  ACATCTACTACACCAAGCTTCAGGGAAAGTCCCTTTTCGGCTTCTCGGGCTCCCACTCCTACAGCCCCAT
  CACCGTGGAGTCGGACTTCAGCAACCCGCTGTATGAAGCTGGGGATACGCGGGAGTATGAAGTTTCCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226207 representing NM_001114099
  Red=Cloning site Green=Tags(s)
  
  MGTPRAQHPPPPQLLFLILLSCPWIQGLPLKEEEILPEPGSETPTVASEALAELLHGALLRRGPEMGYLP
  GPPLGPEGGEEETTTTIITTTTVTTTVTSPVLCNNNISEGEGYVESPDLGSPVSRTLGLLDCTYSIHVYP
  GYGIEIQVQTLNLSQEEELLVLAGGGSPGLAPRLLANSSMLGEGQVLRSPTNRLLLHFQSPRVPRGGGFR
  IHYQAYLLSCGFPPRPAHGDVSVTDLHPGGTATFHCDSGYQLQGEETLICLNGTRPSWNGETPSCMASCG
  GTIHNATLGRIVSPEPGGAVGPNLTCRWVIEAAEGRRLHLHFERVSLDEDNDRLMVRSGGSPLSPVIYDS
  DMDDVPERGLISDAQSLYVELLSETPANPLLLSLRFEAFEEDRCFAPFLAHGNVTTTDPEYRPGALATFS
  CLPGYALEPPGPPNAIECVDPTEPHWNDTEPACKAMCGGELSEPAGVVLSPDWPQSYSPGQDCVWGVHVQ
  EEKRILLQVEILNVREGDMLTLFDGDGPSARVLAQLRGPQPRRRLLSSGPDLTLQFQAPPGPPNPGLGQG
  FVLHFKEVPRNDTCPELPPPEWGWRTASHGDLIRGTVLTYQCEPGYELLGSDILTCQWDLSWSAAPPACQ
  KIMTCADPGEIANGHRTASDAGFPVGSHVQYRCLPGYSLEGAAMLTCYSRDTGTPKWSDRVPKCALKYEP
  CLNPGVPENGYQTLYKHHYQAGESLRFFCYEGFELIGEVTITCVPGHPSQWTSQPPLCKVTQTTDPSRQL
  EGGNLALAILLPLGLVIVLGSGVYIYYTKLQGKSLFGFSGSHSYSPITVESDFSNPLYEAGDTREYEVSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114099
• ORF Size: 2520 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114099.3
• RefSeq Size: 3320 bp
• RefSeq ORF: 2523 bp
• Locus ID: 26470
• UniProt ID: Q6UXD5
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a seizure-related protein that is localized on the cell surface. The gene is located in a region of chromosome 16p11.2 that is thought to contain candidate genes for autism spectrum disorders (ASD), though there is no evidence directly implicating this gene in ASD. Increased expression of this gene has been found in lung cancers, and the protein is therefore considered to be a novel prognostic marker for lung cancer. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]