CSDE1 (NM_001130523) Human Tagged ORF Clone

CAT#: RG226183

CSDE1 (tGFP-tagged) - Human cold shock domain containing E1, RNA-binding (CSDE1), transcript variant 3

Product Images

Circular map for RG226183

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CSDE1
• Synonyms: D1S155E; UNR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226183 representing NM_001130523
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGTTTTTACTGTGTCATCAGATCCTCATCCCTCTCCTGCAGCCCCTCCTTCACTTTCTTTAC
  CCTTATCTTCATCTTCTACCTCATCTGGGACTAAAAAACAAAAGAGGACCCCAACTTATCAGAGATCTAT
  GAGCTTTGATCCAAACCTTCTCCACAACAATGGACATAATGGGTACCCTAATGGTACTTCAGCAGCACTG
  CGTGAAACTGGGGTTATTGAAAAACTGTTAACCTCTTACGGATTTATTCAGTGTTCAGAACGTCAAGCTA
  GACTTTTCTTCCACTGTTCACAGTATAATGGCAACCTGCAAGACTTAAAAGTAGGAGATGATGTTGAATT
  TGAAGTATCATCGGACCGACGGACTGGGAAACCCATTGCTGTTAAACTGGTGAAGATAAAACAAGAAATC
  CTCCCTGAAGAACGAATGAATGGACAAGAAGTGTTTTATCTGACTTACACCCCTGAAGATGTCGAAGGGA
  ACGTTCAGCTGGAAACTGGAGATAAAATAAACTTTGTAATTGATAACAATAAACATACTGGTGCTGTAAG
  TGCTCGCAACATTATGCTGTTGAAAAAGAAACAAGCCCGCTGTCAGGGAGTAGTTTGTGCCATGAAGGAG
  GCATTTGGCTTTATTGAAAGAGGTGATGTTGTAAAAGAGATATTCTTTCACTATAGTGAATTTAAGGGTG
  ACTTAGAAACCTTACAGCCTGGCGATGATGTGGAATTCACAATCAAGGACAGAAATGGTAAAGAAGTTGC
  AACAGATGTCAGACTATTGCCTCAAGGAACAGTCATTTTTGAAGATATCAGCATTGAACATTTTGAAGGA
  ACTGTAACCAAAGTTATCCCAAAAGTACCCAGTAAAAACCAGAATGACCCATTGCCAGGACGCATCAAAG
  TTGACTTTGTGATCCCTAAAGAACTTCCCTTTGGAGACAAAGATACGAAATCCAAGGTGACCCTGCTGGA
  AGGTGACCATGTTAGGTTTAATATTTCAACAGACCGACGTGACAAATTAGAGCGAGCAACCAATATAGAA
  GTTCTGTCAAATACATTTCAGTTCACTAATGAAGCCCGAGAAATGGGTGTGATTGCTGCCATGAGAGATG
  GTTTTGGTTTCATCAAGTGTGTGGATCGTGATGTTCGTATGTTCTTCCACTTCAGTGAAATTCTGGATGG
  GAACCAGCTCCATATTGCAGATGAAGTAGAGTTTACTGTGGTTCCTGATATGCTCTCTGCTCAAAGAAAT
  CATGCTATTAGGATTAAAAAACTTCCCAAGGGCACGGTTTCATTTCATTCCCATTCAGATCACCGTTTTC
  TGGGCACGGTAGAAAAAGAAGCCACTTTTTCCAATCCTAAAACCACTAGCCCAAATAAAGGCAAAGAGAA
  GGAGGCTGAGGATGGCATTATTGCTTATGATGACTGTGGGGTGAAACTGACTATTGCTTTTCAAGCCAAG
  GATGTGGAAGGATCTACTTCTCCTCAAATAGGAGATAAGGTTGAATTTAGTATTAGTGACAAACAGAGGC
  CTGGACAGCAGGTTGCAACTTGTGTGCGACTTTTAGGTCGTAATTCTAACTCCAAGAGGCTCTTGGGTTA
  TGTGGCAACTCTGAAGGATAATTTTGGATTTATTGAAACAGCCAATCATGATAAGGAAATCTTTTTCCAT
  TACAGTGAGTTCTCTGGTGATGTTGATAGCCTGGAACTGGGGGACATGGTCGAGTATAGCTTGTCCAAAG
  GCAAAGGCAACAAAGTCAGTGCAGAAAAAGTGAACAAAACACACTCAGTGAATGGCATTACTGAGGAAGC
  TGATCCCACCATTTACTCTGGCAAAGTAATTCGCCCCCTGAGGAGTGTTGATCCAACACAGACTGAGTAC
  CAAGGAATGATTGAGATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCGTTGGGA
  TGGCCAACAAAGGGGATTGCCTGCAGAAAGGGGAGAGCGTCAAGTTCCAATTGTGTGTCCTGGGCCAAAA
  TGCACAAACTATGGCTTACAACATCACACCCCTGCGCAGGGCCACAGTGGAATGTGTGAAAGATCAGTTT
  GGCTTCATTAACTATGAAGTAGGAGATAGCAAGAAGCTCTTTTTCCATGTGAAAGAAGTTCAGGATGGCA
  TTGAGCTACAGGCAGGAGATGAGGTGGAGTTCTCAGTGATTCTTAATCAGCGCACTGGCAAGTGCAGCGC
  CTGTAATGTTTGGCGAGTCTGTGAGGGCCCCAAGGCTGTTGCAGCTCCTCGACCTGATCGGTTGGTCAAT
  CGCTTGAAGAATATCACTCTGGATGATGCCAGTGCTCCTCGCCTAATGGTTCTTCGTCAGCCAAGGGGAC
  CAGATAACTCAATGGGGTTTGGTGCAGAAAGAAAGATCCGTCAAGCTGGTGTCATTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226183 representing NM_001130523
  Red=Cloning site Green=Tags(s)
  
  MENVFTVSSDPHPSPAAPPSLSLPLSSSSTSSGTKKQKRTPTYQRSMSFDPNLLHNNGHNGYPNGTSAAL
  RETGVIEKLLTSYGFIQCSERQARLFFHCSQYNGNLQDLKVGDDVEFEVSSDRRTGKPIAVKLVKIKQEI
  LPEERMNGQEVFYLTYTPEDVEGNVQLETGDKINFVIDNNKHTGAVSARNIMLLKKKQARCQGVVCAMKE
  AFGFIERGDVVKEIFFHYSEFKGDLETLQPGDDVEFTIKDRNGKEVATDVRLLPQGTVIFEDISIEHFEG
  TVTKVIPKVPSKNQNDPLPGRIKVDFVIPKELPFGDKDTKSKVTLLEGDHVRFNISTDRRDKLERATNIE
  VLSNTFQFTNEAREMGVIAAMRDGFGFIKCVDRDVRMFFHFSEILDGNQLHIADEVEFTVVPDMLSAQRN
  HAIRIKKLPKGTVSFHSHSDHRFLGTVEKEATFSNPKTTSPNKGKEKEAEDGIIAYDDCGVKLTIAFQAK
  DVEGSTSPQIGDKVEFSISDKQRPGQQVATCVRLLGRNSNSKRLLGYVATLKDNFGFIETANHDKEIFFH
  YSEFSGDVDSLELGDMVEYSLSKGKGNKVSAEKVNKTHSVNGITEEADPTIYSGKVIRPLRSVDPTQTEY
  QGMIEIVEEGDMKGEVYPFGIVGMANKGDCLQKGESVKFQLCVLGQNAQTMAYNITPLRRATVECVKDQF
  GFINYEVGDSKKLFFHVKEVQDGIELQAGDEVEFSVILNQRTGKCSACNVWRVCEGPKAVAAPRPDRLVN
  RLKNITLDDASAPRLMVLRQPRGPDNSMGFGAERKIRQAGVID
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130523
• ORF Size: 2439 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130523.3
• RefSeq Size: 4216 bp
• RefSeq ORF: 2442 bp
• Locus ID: 7812
• UniProt ID: O75534
• Cytogenetics: 1p13.2
• Protein Families: Transcription Factors
• Gene Summary: RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.[UniProtKB/Swiss-Prot Function]