CHADL (NM_138481) Human Tagged ORF Clone

CAT#: RG226151

CHADL (tGFP-tagged) - Human chondroadherin-like (CHADL)

Product Images

Circular map for RG226151

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHADL
• Synonyms: SLRR4B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226151 representing NM_138481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGCCCCGGAGCTCCACCCATGTCCCCTTGGTGCTGCCGCTTCTTGTACTTCTGCTGCTGGCCC
  CGGCTAGGCAGGCCGCCGCCCAGCGCTGCCCACAGGCCTGCATCTGTGACAACTCCAGGCGACACGTTGC
  CTGCCGGTACCAGAACCTCACTGAGGTGCCAGACGCCATCCCTGAGCTGACCCAGCGGCTGGACCTGCAG
  GGCAATTTGCTGAAGGTGATCCCCGCAGCCGCCTTCCAGGGCGTGCCTCACCTCACACACCTGGACCTGC
  GCCACTGCGAGGTGGAGCTGGTGGCCGAGGGCGCCTTCCGTGGCCTGGGCCGCCTGCTCCTGCTCAACCT
  GGCCTCCAACCACCTGCGTGAGCTGCCCCAGGAGGCGCTGGACGGGCTGGGCTCGTTGCGGCGGCTGGAG
  CTGGAGGGGAACGCACTGGAGGAGCTGCGGCCGGGGACGTTCGGGGCACTGGGTGCGCTGGCCACGCTAA
  ACCTGGCCCACAACGCCCTGGTTTACCTGCCCGCCATGGCCTTCCAGGGGCTACTGCGCGTCCGCTGGCT
  GCGGCTGTCGCACAACGCGCTCAGCGTGCTGGCCCCCGAGGCCCTGGCTGGCCTGCCCGCCCTGAGACGG
  CTCAGCCTACACCACAACGAGCTCCAGGCTCTGCCCGGGCCTGTCTTGTCCCAGGCCCGCGGCCTGGCCC
  GTCTGGAGCTGGGCCACAACCCGCTCACCTACGCGGGCGAGGAGGACGGGCTGGCGCTGCCCGGCCTGCG
  GGAGCTGCTGCTGGACGGCGGGGCCCTGCAGGCCCTGGGTCCCAGGGCCTTCGCACACTGTCCGCGCCTG
  CACACCCTCGACCTCCGCGGGAACCAGCTAGACACCCTGCCCCCGCTGCAGGGCCCGGGCCAGCTGCGCC
  GGCTGCGGCTGCAGGGGAATCCGCTGTGGTGCGGCTGCCAGGCGCGGCCCCTACTCGAGTGGCTGGCGCG
  GGCGCGCGTGCGCTCGGACGGCGCGTGCCAGGGGCCGCGGCGCCTGCGGGGCGAGGCTCTGGACGCCCTG
  CGGCCCTGGGACCTGCGCTGCCCTGGGGACGCGGCGCAGGAAGAGGAAGAGCTGGAAGAGCGGGCTGTGG
  CCGGGCCCCGCGCCCCTCCGCGCGGCCCTCCGCGCGGCCCCGGGGAGGAGCGGGCAGTCGCGCCTTGCCC
  TCGCGCCTGCGTGTGCGTCCCCGAGTCCCGGCACAGCAGCTGCGAGGGCTGCGGCCTGCAGGCGGTGCCC
  CGCGGCTTCCCCAGCGACACCCAGCTCCTGGACCTGAGGCGGAACCACTTCCCCTCGGTGCCCCGAGCGG
  CCTTCCCCGGCCTGGGCCACCTGGTGTCGCTGCACCTGCAGCACTGCGGCATCGCGGAGCTGGAAGCGGG
  CGCCCTGGCCGGGCTGGGCCGCCTGATCTACCTGTACCTCTCCGACAACCAGCTCGCAGGCCTCAGCGCT
  GCTGCCCTTGAAGGGGCTCCCCGCCTCGGCTACCTGTACCTAGAACGCAACCGTTTCCTGCAGGTGCCAG
  GGGCTGCCCTGCGCGCCCTGCCCAGCCTCTTCTCCCTGCACCTGCAGGACAACGCTGTGGACCGCCTGGC
  ACCTGGGGACCTGGGGAGAACACGGGCCTTGCGCTGGGTCTACCTGAGTGGAAACCGCATCACCGAAGTG
  TCCCTTGGGGCGCTGGGCCCAGCTCGGGAGCTGGAGAAGCTGCACCTGGACAGGAATCAGCTGCGAGAGG
  TGCCCACTGGGGCCTTGGAGGGGCTGCCTGCCCTCCTGGAGCTGCAGCTCTCGGGCAACCCACTCAGGGC
  CTTGCGTGACGGAGCCTTCCAGCCTGTGGGCAGGTCGCTGCAGCACCTCTTCCTGAACAGCAGTGGCCTG
  GAGCAGATTTGTCCTGGGGCCTTTTCAGGCCTGGGGCCCGGGCTCCAGAGCCTGCACCTGCAGAAGAACC
  AGCTTCGGGCCCTGCCTGCCCTGCCCAGTCTCAGCCAGCTGGAGCTCATCGACCTCAGCAGCAATCCCTT
  CCACTGTGACTGCCAGCTGCTTCCGCTGCACAGGTGGCTTACTGGGCTGAACCTGCGGGTGGGGGCCACC
  TGCGCCACCCCTCCCAATGCCCGTGGCCAGAGGGTGAAGGCTGCAGCTGCTGTCTTTGAAGACTGCCCGG
  GCTGGGCTGCCAGAAAGGCCAAGCGGACACCAGCCTCCAGGCCCAGTGCCAGGAGAACCCCCATCAAAGG
  AAGACAGTGTGGAGCAGATAAGGTGGGGAAGGAGAAGGGTCGTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226151 representing NM_138481
  Red=Cloning site Green=Tags(s)
  
  MEGPRSSTHVPLVLPLLVLLLLAPARQAAAQRCPQACICDNSRRHVACRYQNLTEVPDAIPELTQRLDLQ
  GNLLKVIPAAAFQGVPHLTHLDLRHCEVELVAEGAFRGLGRLLLLNLASNHLRELPQEALDGLGSLRRLE
  LEGNALEELRPGTFGALGALATLNLAHNALVYLPAMAFQGLLRVRWLRLSHNALSVLAPEALAGLPALRR
  LSLHHNELQALPGPVLSQARGLARLELGHNPLTYAGEEDGLALPGLRELLLDGGALQALGPRAFAHCPRL
  HTLDLRGNQLDTLPPLQGPGQLRRLRLQGNPLWCGCQARPLLEWLARARVRSDGACQGPRRLRGEALDAL
  RPWDLRCPGDAAQEEEELEERAVAGPRAPPRGPPRGPGEERAVAPCPRACVCVPESRHSSCEGCGLQAVP
  RGFPSDTQLLDLRRNHFPSVPRAAFPGLGHLVSLHLQHCGIAELEAGALAGLGRLIYLYLSDNQLAGLSA
  AALEGAPRLGYLYLERNRFLQVPGAALRALPSLFSLHLQDNAVDRLAPGDLGRTRALRWVYLSGNRITEV
  SLGALGPARELEKLHLDRNQLREVPTGALEGLPALLELQLSGNPLRALRDGAFQPVGRSLQHLFLNSSGL
  EQICPGAFSGLGPGLQSLHLQKNQLRALPALPSLSQLELIDLSSNPFHCDCQLLPLHRWLTGLNLRVGAT
  CATPPNARGQRVKAAAAVFEDCPGWAARKAKRTPASRPSARRTPIKGRQCGADKVGKEKGRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138481
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138481.2
• RefSeq Size: 2533 bp
• RefSeq ORF: 2289 bp
• Locus ID: 150356
• UniProt ID: Q6NUI6
• Cytogenetics: 22q13.2
• Gene Summary: Potential negative modulator of chondrocyte differentiation. Inhibits collagen fibrillogenesis in vitro. May influence chondrocyte's differentiation by acting on its cellular collagenous microenvironment.[UniProtKB/Swiss-Prot Function]