PRR20E (NM_001130407) Human Tagged ORF Clone

CAT#: RG225269

PRR20E (tGFP-tagged) - Human proline rich 20E (PRR20E)

Product Images

Circular map for RG225269

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PRR20E
• Synonyms: PRR20; PRR20A; PRR20B; PRR20C; PRR20D
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG225269 representing NM_001130407
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAACCAAGGCCTTCGAAGCGACTTCGCTCCATGGCCCCTAATCAAGCCTCAGGTGGGCCTCCTC
  CAGAGCCAGGCTGCTGTGTTGCGGACCCTGAAGGCTCCGTGGAAGCAGATGGGCCCGCACAGCCAGCCCA
  ACCCGCAAAACCCATCGCTTACGTGAAACCCTTCAGACGGCAGCCCCCAGCTCGCCCAGAGTCACCCCCT
  CCTGCAGAGAGAGGCCGGCGCCGGGGAGGAAGCCGGCGGCCAGGGCGAGGCCGTGGCAGAAGGGCTGGGC
  CCCGCGGGGACGCTGGCCAGAGACAGGGGGCAGAAGGCTTGATGGCACCGGACGTGCACATCCAACTGGA
  CCACCATGGAGAGCCAGGCCACCAGGGGGAACCGGAAATCACGGAGACCGCAGCCTTCTCCCTTTCTGAA
  ACAGGTCCTCCGCCTGGAACTGTGCAGGAAGGCCCTGGCCCCGACGTGGCGCAACCTGAGCTGGGGTTTC
  AGGAGCCGCCCGCTGCTCCTGGGCCTCAGGCTGTTGACTGGCAACCCGTCTTGACCCTCTATCCCTGCAT
  CGGGTTTAGGGCTCTGGGTGACTCAGCGGTTTTACAAGTCATTCAAACCCCCCAGGGCACCTACGTGCAA
  GGGGTCCCAGTGTTCCTCACCGACATTGCGTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG225269 representing NM_001130407
  Red=Cloning site Green=Tags(s)
  
  MEEPRPSKRLRSMAPNQASGGPPPEPGCCVADPEGSVEADGPAQPAQPAKPIAYVKPFRRQPPARPESPP
  PAERGRRRGGSRRPGRGRGRRAGPRGDAGQRQGAEGLMAPDVHIQLDHHGEPGHQGEPEITETAAFSLSE
  TGPPPGTVQEGPGPDVAQPELGFQEPPAAPGPQAVDWQPVLTLYPCIGFRALGDSAVLQVIQTPQGTYVQ
  GVPVFLTDIAY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130407
• ORF Size: 663 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130407.1, NP_001123879.1
• RefSeq Size: 1847 bp
• RefSeq ORF: 666 bp
• Locus ID: 729250
• UniProt ID: P86496
• Cytogenetics: 13q21.1
• Gene Summary: This gene is one of five identical loci in a cluster on chromosome 13q21.1. The predicted protein is proline-rich and contains several dopamine D4 receptor signatures and PRINTS domains. [provided by RefSeq, Oct 2008]