MAS1 (NM_002377) Human Tagged ORF Clone

CAT#: RG224124

MAS1 (tGFP-tagged) - Human MAS1 oncogene (MAS1)

Product Images

Circular map for RG224124

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAS1
• Synonyms: MAS; MGRA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224124 representing NM_002377
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGGTCAAACGTGACATCATTTGTTGTTGAGGAACCCACGAACATCTCAACTGGCAGGAACGCCT
  CAGTCGGGAATGCACATCGGCAAATCCCCATCGTGCACTGGGTCATTATGAGCATCTCCCCAGTGGGGTT
  TGTTGAGAATGGGATTCTCCTCTGGTTCCTGTGCTTCCGGATGAGAAGAAATCCCTTCACTGTCTACATC
  ACCCACCTGTCTATCGCAGACATCTCACTGCTCTTCTGTATTTTCATCTTGTCTATCGACTATGCTTTAG
  ATTATGAGCTTTCTTCTGGCCATTACTACACAATTGTCACATTATCAGTGACTTTTCTGTTTGGCTACAA
  CACGGGCCTCTATCTGCTGACGGCCATTAGTGTGGAGAGGTGCCTGTCAGTCCTTTACCCCATCTGGTAC
  CGATGCCATCGCCCCAAGTACCAGTCGGCATTGGTCTGTGCCCTTCTGTGGGCTCTTTCTTGCTTGGTGA
  CCACCATGGAGTATGTCATGTGCATCGACAGAGAAGAAGAGAGTCACTCTCGGAATGACTGCCGAGCAGT
  CATCATCTTTATAGCCATCCTGAGCTTCCTGGTCTTCACGCCCCTCATGCTGGTGTCCAGCACCATCTTG
  GTCGTGAAGATCCGGAAGAACACGTGGGCTTCCCATTCCTCCAAGCTTTACATAGTCATCATGGTCACCA
  TCATTATATTCCTCATCTTCGCTATGCCCATGAGACTCCTTTACCTGCTGTACTATGAGTATTGGTCGAC
  CTTTGGGAACCTACACCACATTTCCCTGCTCTTCTCCACAATCAACAGTAGCGCCAACCCTTTCATTTAC
  TTCTTTGTGGGAAGCAGTAAGAAGAAGAGATTCAAGGAGTCCTTAAAAGTTGTTCTGACCAGGGCTTTCA
  AAGATGAAATGCAACCTCGGCGCCAGAAAGACAATTGTAATACGGTCACAGTTGAGACTGTCGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224124 representing NM_002377
  Red=Cloning site Green=Tags(s)
  
  MDGSNVTSFVVEEPTNISTGRNASVGNAHRQIPIVHWVIMSISPVGFVENGILLWFLCFRMRRNPFTVYI
  THLSIADISLLFCIFILSIDYALDYELSSGHYYTIVTLSVTFLFGYNTGLYLLTAISVERCLSVLYPIWY
  RCHRPKYQSALVCALLWALSCLVTTMEYVMCIDREEESHSRNDCRAVIIFIAILSFLVFTPLMLVSSTIL
  VVKIRKNTWASHSSKLYIVIMVTIIIFLIFAMPMRLLYLLYYEYWSTFGNLHHISLLFSTINSSANPFIY
  FFVGSSKKKRFKESLKVVLTRAFKDEMQPRRQKDNCNTVTVETVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002377
• ORF Size: 975 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002377.4
• RefSeq Size: 1135 bp
• RefSeq ORF: 978 bp
• Locus ID: 4142
• UniProt ID: P04201
• Cytogenetics: 6q25.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction, Renin-angiotensin system
• Gene Summary: This gene encodes a class I seven-transmembrane G-protein-coupled receptor. The encoded protein is a receptor for angiotensin-(1-7) and preferentially couples to the Gq protein, activating the phospholipase C signaling pathway. The encoded protein may play a role in multiple processes including hypotension, smooth muscle relaxation and cardioprotection by mediating the effects of angiotensin-(1-7). [provided by RefSeq, May 2012]