IQGAP3 (NM_178229) Human Tagged ORF Clone

CAT#: RG223263

IQGAP3 (tGFP-tagged) - Human IQ motif containing GTPase activating protein 3 (IQGAP3)

Product Images

Circular map for RG223263

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IQGAP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223263 representing NM_178229
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGAGAGCAGCGGGCCCAGGCTGGGCAGCCTATGAACGCCTCACAGCTGAGGAGATGGATGAGC
  AGAGGCGGCAGAATGTTGCCTATCAGTACCTGTGCCGGCTGGAGGAGGCCAAGCGCTGGATGGAGGCCTG
  CCTGAAGGAGGAGCTTCCTTCCCCGGTGGAGCTGGAGGAGAGCCTTCGGAATGGAGTGCTGCTGGCCAAG
  CTGGGCCACTGTTTTGCACCCTCCGTGGTTCCGTTGAAGAAGATCTACGATGTGGAGCAGCTGCGGTACC
  AGGCAACTGGCTTACATTTCCGTCACACAGACAACATCAACTTTTGGCTATCTGCAATAGCCCACATCGG
  TCTGCCTTCGACCTTCTTCCCAGAGACCACGGACATCTATGACAAAAAGAACATGCCCCGGGTAGTCTAC
  TGCATCCATGCTCTCAGTCTCTTCCTCTTCCGGCTGGGATTGGCCCCTCAGATACATGATCTATACGGGA
  AAGTGAAATTCACAGCTGAGGAACTCAGCAACATGGCGTCCGAACTGGCCAAATATGGCCTCCAGCTGCC
  TGCCTTCAGCAAGATCGGGGGCATCTTGGCCAATGAGCTCTCGGTGGATGAGGCTGCAGTCCATGCAGCT
  GTTCTTGCCATCAATGAAGCAGTGGAGCGAGGGGTGGTGGAGGACACCCTGGCTGCCTTGCAGAATCCCA
  GTGCTCTTCTGGAGAATCTCCGAGAGCCTCTGGCAGCCGTCTACCAGGAGATGCTGGCCCAGGCCAAGAT
  GGAGAAGGCAGCCAATGCCAGGAACCATGATGACAGAGAAAGCCAGGACATCTATGACCACTACCTAACT
  CAGGCTGAAATCCAGGGCAATATCAACCATGTCAACGTCCATGGGGCTCTAGAAGTTGTTGATGATGCCC
  TGGAAAGACAGAGCCCTGAAGCCTTGCTCAAGGCCCTTCAAGACCCTGCCCTGGCCCTGCGAGGGGTGAG
  GAGAGACTTTGCTGACTGGTACCTGGAGCAGCTGAACTCAGACAGAGAGCAGAAGGCACAGGAGCTGGGC
  CTGGTGGAGCTTCTGGAAAAGGAGGAAGTCCAGGCTGGTGTGGCTGCAGCCAACACAAAGGGTGATCAGG
  AACAAGCCATGCTCCACGCTGTGCAGCGGATCAACAAAGCCATCCGGAGGGGAGTGGCGGCTGACACTGT
  GAAGGAGCTGATGTGCCCTGAGGCCCAGCTGCCTCCAGTGTACCCTGTTGCATCGTCTATGTACCAGCTG
  GAGCTGGCAGTGCTCCAGCAGCAGCAGGGGGAGCTTGGCCAGGAGGAGCTCTTCGTGGCTGTGGAGATGC
  TCTCAGCTGTGGTCCTGATTAACCGGGCCCTGGAGGCCCGGGATGCCAGTGGCTTCTGGAGCAGCCTGGT
  GAACCCTGCCACAGGCCTGGCTGAGGTGGAAGGAGAAAATGCCCAGCGTTACTTCGATGCCCTGCTGAAA
  TTGCGACAGGAGCGTGGGATGGGTGAGGACTTCCTGAGCTGGAATGACCTGCAGGCCACCGTGAGCCAGG
  TCAATGCACAGACCCAGGAAGAGACTGACCGGGTCCTTGCAGTCAGCCTCATCAATGAGGCTCTGGACAA
  AGGCAGCCCTGAGAAGACTCTGTCTGCCCTACTGCTTCCTGCAGCTGGCCTAGATGATGTCAGCCTCCCT
  GTCGCCCCTCGGTACCATCTCCTCCTTGTGGCAGCCAAAAGGCAGAAGGCCCAGGTGACAGGGGATCCTG
  GAGCTGTGCTGTGGCTTGAGGAGATCCGCCAGGGAGTGGTCAGAGCCAACCAGGACACTAATACAGCTCA
  GAGAATGGCTCTTGGTGTGGCTGCCATCAATCAAGCCATCAAGGAGGGCAAGGCAGCCCAGACTGAGCGG
  GTGTTGAGGAACCCCGCAGTGGCCCTTCGAGGGGTAGTTCCCGACTGTGCCAACGGCTACCAGCGAGCCC
  TGGAAAGTGCCATGGCAAAGAAACAGCGTCCAGCAGACACAGCTTTCTGGGTTCAACATGACATGAAGGA
  TGGCACTGCCTACTACTTCCATCTGCAGACCTTCCAGGGGATCTGGGAGCAACCTCCTGGCTGCCCCCTC
  AACACCTCTCACCTGACCCGGGAGGAGATCCAGTCAGCTGTCACCAAGGTCACTGCTGCCTATGACCGCC
  AACAGCTCTGGAAAGCCAACGTCGGCTTTGTTATCCAGCTCCAGGCCCGCCTCCGTGGCTTCCTAGTTCG
  GCAGAAGTTTGCTGAGCATTCCCACTTTCTGAGGACCTGGCTCCCAGCAGTCATCAAGATCCAGGCTCAT
  TGGCGGGGTTATAGGCAGCGGAAGATTTACCTGGAGTGGTTGCAGTATTTTAAAGCAAACCTGGATGCCA
  TAATCAAGATCCAGGCCTGGGCCCGGATGTGGGCAGCTCGGAGGCAATACCTGAGGCGTCTGCACTACTT
  CCAGAAGAATGTTAACTCCATTGTGAAGATCCAGGCATTTTTCCGAGCCAGGAAAGCCCAAGATGACTAC
  AGGATATTAGTGCATGCACCCCACCCTCCTCTCAGTGTGGTACGCAGATTTGCCCATCTCTTGAATCAAA
  GCCAGCAAGACTTCTTGGCTGAGGCAGAGCTGCTGAAGCTCCAGGAAGAGGTAGTTAGGAAGATCCGATC
  CAATCAGCAGCTGGAGCAGGACCTCAACATCATGGACATCAAGATTGGCCTGCTGGTGAAGAACCGGATC
  ACTCTGCAGGAAGTGGTCTCCCACTGCAAGAAGCTGACCAAGAGGAATAAGGAACAGCTGTCAGATATGA
  TGGTTCTGGACAAGCAGAAGGGTTTAAAGTCGCTGAGCAAAGAGAAACGGCAGAAACTAGAAGCATACCA
  ACACCTCTTCTACCTGCTCCAGACTCAGCCCATCTACCTGGCCAAGCTGATCTTTCAGATGCCACAGAAC
  AAAACCACCAAGTTCATGGAGGCAGTGATTTTCAGCCTGTACAACTATGCCTCCAGCCGCCGAGAGGCCT
  ATCTCCTGCTCCAGCTGTTCAAGACAGCACTCCAGGAGGAAATCAAGTCAAAGGTGGAGCAGCCCCAGGA
  CGTGGTGACAGGCAACCCAACAGTGGTGAGGCTGGTGGTGAGATTCTACCGTAATGGGCGGGGACAGAGT
  GCCCTGCAGGAGATTCTGGGCAAGGTTATCCAGGATGTGCTAGAAGACAAAGTGCTCAGCGTCCACACAG
  ACCCTGTCCACCTCTATAAGAACTGGATCAACCAGACTGAGGCCCAGACAGGGCAGCGCAGCCATCTCCC
  ATATGATGTCACCCCGGAGCAGGCCTTGAGCCACCCCGAGGTCCAGAGACGACTGGACATCGCCCTACGC
  AACCTCCTCGCCATGACTGATAAGTTCCTTTTAGCCATCACCTCATCTGTGGACCAAATTCCGTATGGGA
  TGCGATATGTGGCCAAAGTCCTGAAGGCAACTCTGGCAGAGAAATTCCCTGACGCCACAGACAGCGAGGT
  CTATAAGGTGGTCGGGAACCTCCTGTACTACCGCTTCCTGAACCCAGCTGTGGTGGCTCCTGACGCCTTC
  GACATTGTGGCCATGGCAGCTGGTGGAGCCCTGGCTGCCCCCCAGCGCCATGCCCTGGGGGCTGTGGCTC
  AGCTCCTACAGCACGCTGCGGCTGGCAAGGCCTTCTCTGGGCAGAGCCAGCACCTACGGGTCCTGAATGA
  CTATCTGGAGGAAACACACCTCAAGTTCAGGAAGTTCATCCATAGAGCCTGCCAGGTGCCAGAGCCAGAG
  GAGCGTTTTGCAGTGGACGAGTACTCAGACATGGTGGCTGTGGCCAAACCCATGGTGTACATCACCGTGG
  GGGAGCTGGTCAACACGCACAGGCTGTTGCTGGAGCACCAGGACTGCATTGCCCCTGATCACCAAGACCC
  CCTGCATGAGCTCCTGGAGGATCTTGGGGAGCTGCCCACCATCCCTGACCTTATTGGTGAGAGCATCGCT
  GCAGATGGGCACACGGACCTGAGCAAGCTAGAAGTGTCCCTGACGCTGACCAACAAGTTTGAAGGACTAG
  AGGCAGATGCTGATGACTCCAACACCCGTAGCCTGCTTCTGAGCACCAAGCAGCTGTTGGCCGATATCAT
  ACAGTTCCATCCTGGGGACACCCTCAAGGAGATCCTGTCCCTCTCGGCTTCCAGAGAGCAAGAAGCAGCC
  CACAAGCAGCTGATGAGCCGACGCCAGGCCTGTACAGCCCAGACACCGGAGCCACTGCGACGACACCGCT
  CACTGACAGCTCACTCCCTCCTGCCACTGGCAGAGAAGCAGCGGCGCGTCCTGCGGAACCTACGCCGACT
  TGAAGCCCTGGGGTTGGTCAGCGCCAGAAATGGCTACCAGGGGCTAGTGGACGAGCTGGCCAAGGACATC
  CGCAACCAGCACAGACACAGGCACAGGCGGAAGGCAGAGCTGGTGAAGCTGCAGGCCACATTACAGGGCC
  TGAGCACTAAGACCACCTTCTATGAGGAGCAGGGTGACTACTACAGCCAGTACATCCGGGCCTGCCTGGA
  CCACCTGGCCCCCGACTCCAAGAGTTCTGGGAAGGGGAAGAAGCAGCCTTCTCTTCATTACACTGCTGCT
  CAGCTCCTGGAAAAGGGTGTCTTGGTGGAAATTGAAGATCTTCCCGCCTCTCACTTCAGAAACGTCATCT
  TTGACATCACGCCGGGAGATGAGGCAGGAAAGTTTGAAGTAAATGCCAAGTTCCTGGGTGTGGACATGGA
  GCGATTTCAGCTTCACTATCAGGATCTCCTGCAGCTCCAGTATGAGGGTGTGGCTGTCATGAAACTCTTC
  AACAAGGCCAAAGTCAATGTCAACCTTCTCATCTTCCTCCTCAACAAGAAGTTTTTGCGGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223263 representing NM_178229
  Red=Cloning site Green=Tags(s)
  
  MERRAAGPGWAAYERLTAEEMDEQRRQNVAYQYLCRLEEAKRWMEACLKEELPSPVELEESLRNGVLLAK
  LGHCFAPSVVPLKKIYDVEQLRYQATGLHFRHTDNINFWLSAIAHIGLPSTFFPETTDIYDKKNMPRVVY
  CIHALSLFLFRLGLAPQIHDLYGKVKFTAEELSNMASELAKYGLQLPAFSKIGGILANELSVDEAAVHAA
  VLAINEAVERGVVEDTLAALQNPSALLENLREPLAAVYQEMLAQAKMEKAANARNHDDRESQDIYDHYLT
  QAEIQGNINHVNVHGALEVVDDALERQSPEALLKALQDPALALRGVRRDFADWYLEQLNSDREQKAQELG
  LVELLEKEEVQAGVAAANTKGDQEQAMLHAVQRINKAIRRGVAADTVKELMCPEAQLPPVYPVASSMYQL
  ELAVLQQQQGELGQEELFVAVEMLSAVVLINRALEARDASGFWSSLVNPATGLAEVEGENAQRYFDALLK
  LRQERGMGEDFLSWNDLQATVSQVNAQTQEETDRVLAVSLINEALDKGSPEKTLSALLLPAAGLDDVSLP
  VAPRYHLLLVAAKRQKAQVTGDPGAVLWLEEIRQGVVRANQDTNTAQRMALGVAAINQAIKEGKAAQTER
  VLRNPAVALRGVVPDCANGYQRALESAMAKKQRPADTAFWVQHDMKDGTAYYFHLQTFQGIWEQPPGCPL
  NTSHLTREEIQSAVTKVTAAYDRQQLWKANVGFVIQLQARLRGFLVRQKFAEHSHFLRTWLPAVIKIQAH
  WRGYRQRKIYLEWLQYFKANLDAIIKIQAWARMWAARRQYLRRLHYFQKNVNSIVKIQAFFRARKAQDDY
  RILVHAPHPPLSVVRRFAHLLNQSQQDFLAEAELLKLQEEVVRKIRSNQQLEQDLNIMDIKIGLLVKNRI
  TLQEVVSHCKKLTKRNKEQLSDMMVLDKQKGLKSLSKEKRQKLEAYQHLFYLLQTQPIYLAKLIFQMPQN
  KTTKFMEAVIFSLYNYASSRREAYLLLQLFKTALQEEIKSKVEQPQDVVTGNPTVVRLVVRFYRNGRGQS
  ALQEILGKVIQDVLEDKVLSVHTDPVHLYKNWINQTEAQTGQRSHLPYDVTPEQALSHPEVQRRLDIALR
  NLLAMTDKFLLAITSSVDQIPYGMRYVAKVLKATLAEKFPDATDSEVYKVVGNLLYYRFLNPAVVAPDAF
  DIVAMAAGGALAAPQRHALGAVAQLLQHAAAGKAFSGQSQHLRVLNDYLEETHLKFRKFIHRACQVPEPE
  ERFAVDEYSDMVAVAKPMVYITVGELVNTHRLLLEHQDCIAPDHQDPLHELLEDLGELPTIPDLIGESIA
  ADGHTDLSKLEVSLTLTNKFEGLEADADDSNTRSLLLSTKQLLADIIQFHPGDTLKEILSLSASREQEAA
  HKQLMSRRQACTAQTPEPLRRHRSLTAHSLLPLAEKQRRVLRNLRRLEALGLVSARNGYQGLVDELAKDI
  RNQHRHRHRRKAELVKLQATLQGLSTKTTFYEEQGDYYSQYIRACLDHLAPDSKSSGKGKKQPSLHYTAA
  QLLEKGVLVEIEDLPASHFRNVIFDITPGDEAGKFEVNAKFLGVDMERFQLHYQDLLQLQYEGVAVMKLF
  NKAKVNVNLLIFLLNKKFLRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178229
• ORF Size: 4893 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178229.3, NP_839943.2
• RefSeq Size: 6069 bp
• RefSeq ORF: 4896 bp
• Locus ID: 128239
• UniProt ID: Q86VI3
• Cytogenetics: 1q22
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton