PCDH7 (NM_032457) Human Tagged ORF Clone

CAT#: RG223033

PCDH7 (tGFP-tagged) - Human protocadherin 7 (PCDH7), transcript variant c

Product Images

Circular map for RG223033

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PCDH7
• Synonyms: BH-Pcdh; BHPCDH; PPP1R120
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223033 representing NM_032457
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGATGCGGACCGCGGGATGGGCGCGCGGCTGGTGCTTGGGCTGCTGCCTCCTCCTGCCGCTCT
  CGCTCAGCCTGGCGGCCGCCAAGCAGCTCCTCCGGTACCGGCTGGCCGAGGAGGGCCCCGCCGACGTCCG
  CATCGGCAACGTGGCTTCAGACCTGGGCATCGTGACCGGATCGGGTGAGGTGACTTTCAGCCTGGAGTCC
  GGTTCCGAGTACCTGAAGATCGACAACCTCACTGGCGAGCTGAGCACGAGCGAGCGGCGCATCGACCGCG
  AGAAGCTGCCCCAGTGTCAGATGATCTTCGACGAGAACGAGTGCTTCCTGGACTTCGAGGTGTCGGTGAT
  CGGGCCCTCGCAGAGCTGGGTGGACCTGTTTGAGGGTCAGGTCATCGTGCTTGACATCAACGACAACACG
  CCCACCTTCCCGTCGCCCGTGCTCACGCTCACGGTGGAGGAGAATCGGCCGGTGGGCACACTTTACCTGC
  TGCCCACAGCCACCGACCGCGACTTCGGCCGCAACGGCATCGAGCGCTACGAGCTGCTCCAGGAGCCCGG
  AGGCGGCGGCAGCGGCGGCGAGAGCCGGCGCGCCGGGGCGGCCGACAGCGCCCCCTACCCCGGGGGCGGC
  GGGAACGGCGCGAGCGGCGGCGGCTCGGGAGGCTCCAAGCGGCGGCTGGACGCATCAGAGGGCGGCGGCG
  GCACCAACCCCGGCGGCCGCAGCAGCGTGTTCGAGCTGCAGGTGGCGGACACCCCGGACGGCGAGAAGCA
  GCCGCAGCTGATCGTGAAGGGCGTGTACGAGGCCGACTTGGCTGAGAACAGCGCCCCGGGGACCCCCATC
  CTGCAACTGCGCGCAGCCGACTTGGACGTGGGGGTCAACGGGCAGATCGAATACGTGTTCGGGGCGGCCA
  CCGAGTCGGTGAGGCGGCTGCTGCGCCTTGACGAGACGTCCGGCTGGCTCAGCGTCCTGCACCGGATCGA
  CCGCGAGGAGGTGAACCAGCTGCGCTTCACGGTCATGGCCCGCGACCGCGGGCAGCCCCCCAAGACCGAC
  AAGGCCACCGTGGTCCTTAACATCAAAGACGAGAACGACAACGTGCCGTCCATTGAAATCCGCAAGATTG
  GGCGCATCCCCCTCAAGGACGGGGTGGCCAACGTGGCCGAGGACGTTCTGGTCGACACCCCCATCGCTCT
  GGTGCAGGTGTCCGACCGAGACCAAGGCGAGAACGGGGTGGTCACCTGCACCGTGGTGGGCGACGTGCCC
  TTCCAGCTCAAGCCAGCCAGCGACACCGAGGGCGACCAGAACAAGAAAAAGTACTTCTTGCACACCTCGA
  CCCCTCTGGACTATGAGGCCACCCGGGAGTTCAACGTGGTCATCGTGGCGGTGGACTCAGGCAGCCCCAG
  CCTCTCGAGCAACAACTCCCTGATTGTCAAGGTGGGAGACACCAACGACAACCCGCCCATGTTCGGCCAG
  TCGGTGGTGGAGGTTTACTTCCCTGAGAACAACATCCCGGGCGAGAGGGTGGCCACGGTGCTGGCGACAG
  ACGCAGACAGCGGTAAGAACGCCGAGATCGCCTACTCGCTGGACTCCTCTGTGATGGGGATCTTTGCCAT
  CGATCCCGATTCTGGGGACATCCTGGTCAATACCGTGCTGGACCGCGAGCAGACTGACAGGTATGAGTTT
  AAAGTTAACGCCAAAGACAAAGGCATCCCCGTGCTGCAGGGCAGCACTACGGTGATTGTGCAGGTGGCTG
  ATAAAAATGACAATGACCCTAAGTTTATGCAGGACGTCTTCACCTTTTATGTGAAAGAAAACTTGCAGCC
  CAACAGCCCTGTGGGGATGGTCACCGTGATGGATGCTGACAAGGGGCGGAATGCAGAGATGAGCCTGTAC
  ATAGAGGAGAACAATAACATTTTTTCTATTGAAAATGACACGGGGACCATTTACTCCACAATGTCTTTTG
  ACCGGGAACATCAGACCACATACACTTTCAGAGTCAAGGCTGTGGATGGGGGAGATCCTCCCAGATCTGC
  CACAGCTACAGTCTCGCTTTTTGTGATGGATGAAAATGACAATGCTCCCACAGTTACCCTTCCCAAAAAC
  ATTTCCTACACTTTACTGCCACCTTCGAGTAATGTCAGGACAGTAGTAGCTACAGTGTTGGCAACAGACA
  GTGATGATGGCATCAATGCAGACCTGAACTACAGCATTGTGGGAGGAAATCCCTTCAAGCTGTTTGAAAT
  TGATCCCACTAGTGGTGTGGTTTCCTTAGTGGGAAAACTCACCCAAAAGCATTATGGCTTGCACAGGTTG
  GTGGTGCAAGTGAATGACAGTGGGCAGCCTTCCCAGTCCACCACGACTCTGGTGCACGTGTTTGTCAATG
  AAAGTGTTTCTAATGCAACTGCGATTGACTCCCAGATAGCTAGAAGTTTGCACATCCCACTCACCCAGGA
  TATAGCTGGTGACCCAAGCTATGAAATTAGCAAACAGAGACTCAGTATTGTCATTGGCGTGGTTGCTGGC
  ATTATGACGGTGATTCTAATCATCTTAATTGTAGTGATGGCAAGGTACTGCAGGTCCAAAAATAAAAATG
  GCTATGAAGCCGGCAAAAAAGATCACGAAGACTTTTTTACACCCCAACAGCATGACAAATCTAAAAAGCC
  TAAAAAGGACAAGAAAAACAAAAAATCTAAGCAGCCTCTCTACAGCAGCATTGTCACTGTGGAGGCTTCT
  AAGCCAAATGGACAGAGGTATGATAGTGTCAATGAGAAGCTGTCAGACAGCCCAAGCATGGGGCGATACA
  GGTCCGTTAATGGTGGGCCCGGCAGTCCTGACCTGGCAAGGCATTACAAATCTAGTTCCCCATTGCCTAC
  TGTTCAGCTTCATCCCCAGTCACCAACTGCAGGAAAAAAACACCAGGCCGTACAAGATCTACCACCAGCC
  AACACATTTGTGGGAGCAGGAGACAACATTTCAATTGGATCAGATCACTGCTCTGAGTACAGCTGTCAAA
  CCAATAACAAGTACAGCAAACAGCCATTTCGTAGAGTGACGTTTTCTGTTGTGAGTCAGCCTCAGGACCC
  ACATCAGGGGTCACTGCAGAGTTGCTATGACAGCGGGCTGGAGGAGTCAGAAACACCAAGCAGTAAGAGT
  TCATCAGGGCCAAGACTGGGTGCGCTTCCACTCCCAGAGGACAACTATGAAAGGACCACGCCGGATGGCA
  GTGTTGATTCAAGGCCTCTTCCAGATGTAGCCCTGACTGGGAAGTGCACTCGTGAGTGTGATGAGTATGG
  CCACTCAGACTCCTGCTGGATGCCGGTCCGCACTTCTCCGGAGAGGAAGAAGAGCCAGCCTAAACTGTCC
  ACTTTCATGCCTGTTGATGAACGAGGAAGCCAGGAAAAGCTGGCCAATGGGGAGGCCGCCATCATGGGTG
  ACCGCAACAGAAACCTCCTGAACAAAAAGTTGACCTCATCCTATGAGACCTTCAGTGCAGCTAGTTTCAG
  CAAAAATGAGGAAGCCAACCCTGAGGATATTCCCCTTACAAAAACAGGGGAATATAAGCCATCTCCTGTC
  AATACTCTCACTAGAAGAGAAGTTTACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223033 representing NM_032457
  Red=Cloning site Green=Tags(s)
  
  MLRMRTAGWARGWCLGCCLLLPLSLSLAAAKQLLRYRLAEEGPADVRIGNVASDLGIVTGSGEVTFSLES
  GSEYLKIDNLTGELSTSERRIDREKLPQCQMIFDENECFLDFEVSVIGPSQSWVDLFEGQVIVLDINDNT
  PTFPSPVLTLTVEENRPVGTLYLLPTATDRDFGRNGIERYELLQEPGGGGSGGESRRAGAADSAPYPGGG
  GNGASGGGSGGSKRRLDASEGGGGTNPGGRSSVFELQVADTPDGEKQPQLIVKGVYEADLAENSAPGTPI
  LQLRAADLDVGVNGQIEYVFGAATESVRRLLRLDETSGWLSVLHRIDREEVNQLRFTVMARDRGQPPKTD
  KATVVLNIKDENDNVPSIEIRKIGRIPLKDGVANVAEDVLVDTPIALVQVSDRDQGENGVVTCTVVGDVP
  FQLKPASDTEGDQNKKKYFLHTSTPLDYEATREFNVVIVAVDSGSPSLSSNNSLIVKVGDTNDNPPMFGQ
  SVVEVYFPENNIPGERVATVLATDADSGKNAEIAYSLDSSVMGIFAIDPDSGDILVNTVLDREQTDRYEF
  KVNAKDKGIPVLQGSTTVIVQVADKNDNDPKFMQDVFTFYVKENLQPNSPVGMVTVMDADKGRNAEMSLY
  IEENNNIFSIENDTGTIYSTMSFDREHQTTYTFRVKAVDGGDPPRSATATVSLFVMDENDNAPTVTLPKN
  ISYTLLPPSSNVRTVVATVLATDSDDGINADLNYSIVGGNPFKLFEIDPTSGVVSLVGKLTQKHYGLHRL
  VVQVNDSGQPSQSTTTLVHVFVNESVSNATAIDSQIARSLHIPLTQDIAGDPSYEISKQRLSIVIGVVAG
  IMTVILIILIVVMARYCRSKNKNGYEAGKKDHEDFFTPQQHDKSKKPKKDKKNKKSKQPLYSSIVTVEAS
  KPNGQRYDSVNEKLSDSPSMGRYRSVNGGPGSPDLARHYKSSSPLPTVQLHPQSPTAGKKHQAVQDLPPA
  NTFVGAGDNISIGSDHCSEYSCQTNNKYSKQPFRRVTFSVVSQPQDPHQGSLQSCYDSGLEESETPSSKS
  SSGPRLGALPLPEDNYERTTPDGSVDSRPLPDVALTGKCTRECDEYGHSDSCWMPVRTSPERKKSQPKLS
  TFMPVDERGSQEKLANGEAAIMGDRNRNLLNKKLTSSYETFSAASFSKNEEANPEDIPLTKTGEYKPSPV
  NTLTRREVYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032457
• ORF Size: 3600 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032457.2
• RefSeq Size: 8704 bp
• RefSeq ORF: 3744 bp
• Locus ID: 5099
• Cytogenetics: 4p15.1
• Domains: CA
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a protein with an extracellular domain containing 7 cadherin repeats. The gene product is an integral membrane protein that is thought to function in cell-cell recognition and adhesion. Alternative splicing yields isoforms with unique cytoplasmic tails. [provided by RefSeq, Jul 2008]