CELF2 (NM_001025077) Human Tagged ORF Clone

CAT#: RG222915

CELF2 (tGFP-tagged) - Human CUGBP, Elav-like family member 2 (CELF2), transcript variant 3

Product Images

Circular map for RG222915

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CELF2
• Synonyms: BRUNOL3; CELF-2; CUG-BP2; CUGBP2; ETR-3; ETR3; NAPOR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222915 representing NM_001025077
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGTCCCAAATCCGCTGTTACTATGAGAAATGAAGAGCTGCTTTTAAGTAACGGCACAGCCAACA
  AGATGAACGGAGCTTTGGATCACTCAGACCAACCAGACCCAGATGCCATTAAGATGTTTGTCGGACAGAT
  CCCCCGGTCATGGTCGGAAAAGGAGCTGAAAGAACTTTTTGAGCCTTACGGAGCCGTCTACCAGATCAAC
  GTCCTCCGGGACCGGAGTCAGAACCCTCCGCAGAGTAAAGGTTGTTGTTTCGTAACATTTTATACAAGAA
  AAGCTGCACTTGAGGCCCAGAATGCACTGCACAATATTAAAACTTTACCTGGGATGCATCATCCCATTCA
  GATGAAACCTGCAGATAGTGAAAAGTCCAACGCTGTGGAAGACAGAAAATTGTTCATAGGAATGGTATCG
  AAGAAATGTAATGAGAACGACATCAGGGTGATGTTCTCTCCATTTGGCCAGATAGAAGAATGCCGGATCC
  TCCGGGGACCTGATGGGCTGAGTCGAGGCTGTGCGTTTGTCACATTTTCTACAAGGGCAATGGCACAGAA
  TGCAATCAAAGCCATGCATCAGTCTCAGACCATGGAGGGCTGCTCTTCACCTATCGTGGTGAAGTTTGCT
  GACACTCAGAAGGACAAAGAGCAAAGGCGCCTCCAGCAGCAGCTCGCTCAGCAGATGCAGCAGCTCAACA
  CTGCCACCTGGGGGAACCTGACAGGGCTGGGCGGACTGACCCCACAGTATCTGGCGCTCCTGCAGCAGGC
  CACCTCCTCCAGCAACCTGGGTGCGTTCAGCGGCATTCAACAAATGGCAGGCATGAATGCTTTACAGTTG
  CAGAACCTGGCGACGCTGGCTGCTGCTGCAGCTGCGGCCCAGACCTCAGCCACCAGCACCAATGCAAACC
  CTCTCTCTACCACGAGCAGCGCCCTGGGAGCCCTCACGAGTCCCGTGGCTGCTTCAACCCCCAACTCCAC
  TGCTGGTGCAGCCATGAACTCCTTGACCTCTCTCGGGACTCTGCAAGGACTGGCTGGAGCCACTGTTGGA
  CTGAATAATATTAATGCACTAGCAGGTATGGCGGCTCTGAATGGAGGACTTGGCGCCACAGGCTTGACGA
  ATGGCACGGCTGGCACCATGGACGCCCTCACCCAGGCCTACTCAGGAATTCAACAGTACGCAGCCGCCGC
  GCTGCCCACTCTGTACAGCCAGAGCCTGCTGCAGCAGCAGAGCGCTGCAGGCAGCCAGAAGGAAGGTCCA
  GAGGGGGCAAACCTCTTTATTTACCACCTTCCACAGGAATTTGGAGACCAGGACATTCTGCAGATGTTCA
  TGCCTTTTGGAAATGTTATCTCTGCTAAAGTCTTCATTGACAAACAGACCAATCTGAGCAAGTGCTTTGG
  TTTTGTTAGCTACGACAATCCAGTCTCTGCACAAGCTGCTATCCAAGCTATGAATGGCTTTCAGATCGGC
  ATGAAACGCTTGAAGGTGCAGCTGAAGCGTTCCAAAAACGACAGCAAACCTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222915 representing NM_001025077
  Red=Cloning site Green=Tags(s)
  
  MRCPKSAVTMRNEELLLSNGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELFEPYGAVYQIN
  VLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKPADSEKSNAVEDRKLFIGMVS
  KKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFSTRAMAQNAIKAMHQSQTMEGCSSPIVVKFA
  DTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQL
  QNLATLAAAAAAAQTSATSTNANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVG
  LNNINALAGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGP
  EGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQAMNGFQIG
  MKRLKVQLKRSKNDSKPY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025077
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025077.3
• RefSeq Size: 9227 bp
• RefSeq ORF: 1527 bp
• Locus ID: 10659
• UniProt ID: O95319
• Cytogenetics: 10p14
• Gene Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]