CHMP7 (NM_152272) Human Tagged ORF Clone

CAT#: RG222461

CHMP7 (tGFP-tagged) - Human CHMP family, member 7 (CHMP7)

Product Images

Circular map for RG222461

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CHMP7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222461 representing NM_152272
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTCCCCGGAGCGGGAGGCCGAGGCCCCAGCCGGGGGAGACCCGGCGGGCCTTCTGCCCCCCGAGT
  GGGAGGAGGACGAGGAGCGCATGTCCTTCCTGTTCTCCGCTTTCAAGAGGAGTCGCGAGGTGAACAGCAC
  CGACTGGGACAGCAAGATGGGCTTCTGGGCGCCGTTGGTGCTGAGCCACAGCCGCCGCCAGGGGGTGGTG
  CGCCTGCGTCTGCGGGACTTGCAGGAGGCCTTTCAGCGCAAGGGGAGCGTCCCGCTGGGGCTGGCCACGG
  TGCTGCAGGACCTGCTGCGTCGAGGGGAGCTGCAGCGGGAGTCAGACTTCATGGCCAGTGTAGACAGCAG
  CTGGATCTCCTGGGGGGTTGGGGTCTTCCTGCTGAAGCCTCTCAAGTGGACTCTTTCTAACATGCTGGGA
  GATAATAAGGTTCCAGCTGAGGAGGTCCTTGTCGCTGTGGAGCTGTTGAAGGAAAAGGCTGAGGAGGTGT
  ATCGTCTGTATCAGAACTCGCCCCTCTCCTCCCACCCCGTGGTGGCCCTGTCAGAGCTCAGCACCCTCTG
  TGCTAACTCCTGCCCAGATGAGAGGACCTTCTACTTGGTGTTGCTGCAGCTGCAGAAGGAGAAGAGGGTC
  ACAGTCCTCGAGCAGAACGGGGAGAAGATTGTGAAGTTTGCCCGAGGGCCACGTGCCAAGGTCTCTCCAG
  TCAATGACGTAGATGTTGGGGTGTACCAGCTGATGCAGAGTGAACAGCTTCTCTCACGCAAAGTGGAGTC
  CTTATCCCAGGAAGCAGAGAGGTGTAAAGAAGAAGCCCGCCGGGCATGCCGAGCAGGAAAGAAGCAGCTG
  GCACTGAGGTCTCTCAAGGCCAAGCAACGGACAGAGAAGCGCATCGAGGCCTTGCATGCCAAGCTGGACA
  CTGTTCAAGGCATCCTGGACCGGATCTATGCCTCCCAGACAGATCAGATGGTTTTTAACGCCTACCAGGC
  TGGGGTAGGAGCACTCAAACTCTCCATGAAGGATGTCACAGTGGAGAAGGCAGAGAGCCTCGTGGATCAG
  ATCCAAGAGCTCTGTGACACCCAGGATGAAGTTTCTCAGACTCTGGCTGGTGGGGTAACAAATGGCTTAG
  ATTTTGACAGTGAAGAACTGGAGAAGGAATTGGACATCCTCCTTCAGGATACCACCAAAGAACCTTTGGA
  TCTGCCTGACAACCCCCGCAATAGGCATTTTACCAACAGCGTGCCTAACCCTAGGATCTCAGATGCTGAA
  CTTGAAGCTGAACTTGAGAAACTGTCCTTATCAGAGGGAGGTTTGGTCCCAAGCAGTAAATCTCCAAAAA
  GGCAATTGGAACCGACTCTAAAGCCATTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222461 representing NM_152272
  Red=Cloning site Green=Tags(s)
  
  MWSPEREAEAPAGGDPAGLLPPEWEEDEERMSFLFSAFKRSREVNSTDWDSKMGFWAPLVLSHSRRQGVV
  RLRLRDLQEAFQRKGSVPLGLATVLQDLLRRGELQRESDFMASVDSSWISWGVGVFLLKPLKWTLSNMLG
  DNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVALSELSTLCANSCPDERTFYLVLLQLQKEKRV
  TVLEQNGEKIVKFARGPRAKVSPVNDVDVGVYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQL
  ALRSLKAKQRTEKRIEALHAKLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQ
  IQELCDTQDEVSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAE
  LEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152272
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152272.5
• RefSeq Size: 3420 bp
• RefSeq ORF: 1362 bp
• Locus ID: 91782
• UniProt ID: Q8WUX9
• Cytogenetics: 8p21.3
• Domains: SNF7
• Gene Summary: ESCRT-III-like protein required to recruit the ESCRT-III complex to the nuclear envelope during late anaphase (PubMed:26040712). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Plays a role in the endosomal sorting pathway (PubMed:16856878).[UniProtKB/Swiss-Prot Function]