HLTF (NM_139048) Human Tagged ORF Clone

CAT#: RG222346

HLTF (tGFP-tagged) - Human helicase-like transcription factor (HLTF), transcript variant 2

Product Images

Circular map for RG222346

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HLTF
• Synonyms: HIP116; HIP116A; HLTF1; RNF80; SMARCA3; SNF2L3; ZBU1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222346 representing NM_139048
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAA
  ATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGA
  CTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTA
  CGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATG
  ATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGG
  TGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAAT
  GCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGT
  TGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGG
  CTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTT
  AAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTG
  AGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAG
  CAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAG
  AAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACGG
  CCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCT
  ACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCA
  GATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTA
  AGTTTCGCATGTCAGAATTGTCTAGCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAG
  CAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAG
  TCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCAT
  TAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAA
  GAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGG
  ATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATC
  GTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGA
  CTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACAT
  GCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTT
  TGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACC
  ATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTT
  AGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAAC
  CTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGAT
  TTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCA
  CATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAA
  ATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAA
  GATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCT
  GTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCAC
  ATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATT
  AGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCAC
  GCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCC
  TGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCA
  AAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTG
  TCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCT
  GGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCAT
  CACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTT
  GCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCA
  GAACATTAATTGACTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222346 representing NM_139048
  Red=Cloning site Green=Tags(s)
  
  MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGL
  RYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANN
  AFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQL
  KTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSE
  KDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKA
  DGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQ
  SETKGRAKAGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNW
  IDQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGH
  AIRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGL
  RRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLA
  HYADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVP
  VITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMH
  ALTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLL
  SLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKREL
  AAGAFGTKKPNADEMKQAKINEIRTLIDL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139048
• ORF Size: 3027 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139048.3
• RefSeq Size: 4480 bp
• RefSeq ORF: 3030 bp
• Locus ID: 6596
• UniProt ID: Q14527
• Cytogenetics: 3q24
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]