MTMR3 (NM_021090) Human Tagged ORF Clone

CAT#: RG222287

MTMR3 (tGFP-tagged) - Human myotubularin related protein 3 (MTMR3), transcript variant 3

Product Images

Circular map for RG222287

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MTMR3
• Synonyms: FYVE-DSP1; ZFYVE10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222287 representing NM_021090
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAGAGACTCGGCACAGCCTTGAGTGCATCCAGGCCAATCAGATCTTTCCCAGGAAGCAGCTGA
  TCCGGGAGGATGAGAATCTTCAGGTTCCTTTCCTTGAACTTCATGGAGAGAGCACAGAGTTTGTGGGCCG
  TGCCGAGGATGCCATCATTGCCCTTTCCAATTACAGACTTCACATCAAGTTCAAGGAGTCTCTTGTTAAT
  GTTCCATTACAGCTTATAGAAAGTGTTGAATGCCGAGATATATTTCAGCTTCATTTGACTTGCAAAGACT
  GCAAAGTTATCAGGTGTCAGTTTTCAACCTTTGAGCAGTGTCAAGAGTGGCTGAAGAGACTGAACAACGC
  AATCCGACCACCTGCTAAAATAGAAGATCTCTTCTCATTTGCATACCATGCTTGGTGCATGGAGGTCTAT
  GCCAGTGAAAAAGAGCAACATGGAGACCTGTGCAGACCAGGGGAGCATGTAACTTCAAGGTTTAAAAACG
  AGGTGGAGAGGATGGGTTTTGATATGAACAACGCCTGGAGGATTTCCAACATCAATGAGAAGTACAAATT
  ATGTGGTAGCTATCCTCAAGAGCTCATAGTGCCTGCCTGGATCACTGACAAAGAACTGGAAAGTGTATCA
  AGTTTCAGGTCCTGGAAGCGCATCCCTGCCGTCATCTACAGGCACCAGAGCAATGGAGCTGTCATTGCCC
  GCTGTGGACAGCCAGAGGTTAGCTGGTGGGGCTGGCGAAATGCAGATGATGAGCATCTGGTACAGTCAGT
  AGCCAAAGCTTGTGCCTCTGACTCCCGATCGAGTGGCAGCAAGCTGTCAACTAGGAACACTTCTCGAGAC
  TTTCCCAATGGGGGAGACCTTTCTGACGTGGAGTTCGATTCTTCTCTGTCAAATGCTTCAGGAGCAGAGA
  GTTTAGCCATCCAACCGCAGAAGCTTTTGATCTTGGATGCACGCTCCTATGCAGCTGCTGTGGCAAACCG
  AGCCAAAGGAGGAGGCTGCGAATGCCCAGAGTATTACCCAAACTGTGAAGTTGTGTTTATGGGGATGGCA
  AACATTCATTCTATTCGGAGGAGTTTTCAGTCTCTGCGGTTGCTGTGCACTCAGATGCCAGATCCGGGAA
  ATTGGCTATCAGCTCTTGAAAGCACAAAATGGCTCCATCACTTGTCTGTGCTTCTGAAATCAGCGCTTCT
  GGTAGTGCATGCTGTGGATCAGGATCAGCGGCCGGTGCTAGTACACTGCTCAGATGGCTGGGACCGCACC
  CCCCAGATTGTGGCATTGGCTAAGCTCTTGCTGGACCCTTATTACCGAACCATAGAGGGTTTCCAGGTCC
  TCGTGGAAATGGAGTGGCTGGATTTTGGCCATAAATTTGCTGACCGGTGTGGTCATGGGGAGAACTCGGA
  TGATCTGAATGAACGTTGCCCAGTGTTTCTGCAGTGGCTTGACTGTGTTCATCAGCTTCAGAGGCAATTT
  CCTTGCTCTTTTGAGTTCAATGAAGCATTCCTTGTGAAACTGGTGCAGCATACCTATTCCTGCCTGTTTG
  GAACATTCCTGTGCAACAACGCCAAGGAGAGAGGGGAAAAGCATACTCAGGAACGGACATGTTCCGTGTG
  GTCACTTCTTCGGGCAGGCAACAAGGCTTTCAAAAACCTACTGTATTCCTCTCAGTCAGAAGCCGTGCTG
  TACCCTGTGTGCCATGTGCGTAACCTGATGCTGTGGAGTGCAGTGTACCTGCCCTGCCCATCCCCAACCA
  CCCCTGTGGACGACAGCTGTGCACCATACCCAGCCCCAGGCACCAGCCCTGATGATCCCCCCCTGAGCCG
  GCTACCAAAGACTAGATCATACGACAATCTGACCACAGCCTGTGACAACACAGTGCCTCTGGCCAGCCGG
  CGCTGCAGCGACCCCAGCCTGAACGAGAAGTGGCAGGAGCACCGGCGCTCACTAGAGCTGAGCAGCCTGG
  CTGGCCCTGGAGAGGATCCCCTTTCTGCCGACAGCCTAGGGAAGCCCACCAGAGTGCCGGGGGGTGCCGA
  GCTTTCTGTTGCAGCCGGAGTAGCTGAGGGGCAGATGGAGAACATCTTGCAGGAGGCCACCAAAGAGGAG
  AGTGGAGTAGAGGAACCTGCCCACAGGGCAGGCATTGAGATACAGGAGGGTAAAGAGGACCCTCTCTTAG
  AAAAGGAGAGCAGGAGGAAGACACCTGAGGCCTCAGCCATTGGACTTCACCAAGACCCAGAACTGGGTGA
  TGCTGCTCTGAGGAGCCATCTGGATATGAGCTGGCCTCTGTTCTCACAGGGCATTTCTGAACAGCAGAGT
  GGGCTCAGTGTTCTCCTCAGTTCTCTCCAGGTCCCCCCCAGGGGAGAGGATTCCCTGGAGGTCCCTGTGG
  AGCAGTTTCGAATAGAAGAGATTGCAGAGGGTAGGGAGGAAGCAGTTCTTCCAATCCCAGTAGATGCAAA
  AGTTGGCTATGGTACCTCACAGTCATGTTCTCTGCTACCTTCCCAAGTCCCTTTTGAGACCAGAGGACCA
  AACGTGGACAGTTCTACAGACATGTTAGTGGAAGATAAGGTGAAGTCAGTAAGTGGGCCCCAAGGTCATC
  ATAGATCTTGCCTTGTAAATAGTGGCAAGGACAGGCTTCCTCAGACCATGGAACCCAGCCCTTCAGAGAC
  AAGCCTGGTCGAGAGGCCCCAAGTGGGGTCTGTGGTGCATAGGACTTCCCTTGGCAGCACTCTCAGCCTG
  ACACGTTCCCCTTGTGCCTTGCCTTTAGCCGAATGTAAAGAGGGGCTTGTGTGCAATGGTGCCCCAGAGA
  CTGAAAACAGGGCCTCAGAGCAGCCCCCAGGTCTTAGCACCCTCCAGATGTACCCCACACCCAATGGGCA
  TTGCGCCAATGGGGAGGCTGGTAGGAGCAAGGACTCACTGAGCCGTCAGCTGTCTGCTATGAGCTGCAGC
  TCTGCCCACTTACACTCAAGGAACTTGCACCACAAGTGGCTGCATAGCCACTCAGGAAGGCCATCTGCAA
  CCAGCAGCCCCGACCAGCCTTCCCGCAGCCACCTGGACGATGATGGCATGTCAGTGTACACAGACACGAT
  CCAACAGCGCCTGCGTCAGATTGAGTCAGGCCACCAGCAGGAAGTAGAAACTTTGAAGAAACAAGTCCAG
  GAGCTGAAGAGTCGCCTGGAGAGCCAGTACCTGACCAGCTCCCTACACTTTAATGGAGACTTTGGGGATG
  AGGTGACTTCAATCCCCGACTCGGAAAGCAATCTGGATCAGAACTGTTTGTCTCGCTGCAGCACAGAGAT
  TTTCTCTGAAGCCAGCTGGGAGCAGGTGGATAAACAGGACACAGAGATGACCCGTTGGCTTCCTGACCAC
  CTGGCCGCCCACTGCTATGCGTGCGACAGTGCCTTCTGGCTTGCCAGCAGGAAGCACCACTGCAGGAATT
  GTGGGAACGTATTCTGCTCCAGTTGTTGTAACCAGAAGGTTCCAGTTCCCAGCCAGCAGCTCTTTGAACC
  CAGTCGAGTATGCAAGTCTTGCTATAGCAGCCTACATCCCACAAGCTCCAGCATTGACCTTGAACTGGAT
  AAGCCCATTGCTGCCACTTCCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222287 representing NM_021090
  Red=Cloning site Green=Tags(s)
  
  MDEETRHSLECIQANQIFPRKQLIREDENLQVPFLELHGESTEFVGRAEDAIIALSNYRLHIKFKESLVN
  VPLQLIESVECRDIFQLHLTCKDCKVIRCQFSTFEQCQEWLKRLNNAIRPPAKIEDLFSFAYHAWCMEVY
  ASEKEQHGDLCRPGEHVTSRFKNEVERMGFDMNNAWRISNINEKYKLCGSYPQELIVPAWITDKELESVS
  SFRSWKRIPAVIYRHQSNGAVIARCGQPEVSWWGWRNADDEHLVQSVAKACASDSRSSGSKLSTRNTSRD
  FPNGGDLSDVEFDSSLSNASGAESLAIQPQKLLILDARSYAAAVANRAKGGGCECPEYYPNCEVVFMGMA
  NIHSIRRSFQSLRLLCTQMPDPGNWLSALESTKWLHHLSVLLKSALLVVHAVDQDQRPVLVHCSDGWDRT
  PQIVALAKLLLDPYYRTIEGFQVLVEMEWLDFGHKFADRCGHGENSDDLNERCPVFLQWLDCVHQLQRQF
  PCSFEFNEAFLVKLVQHTYSCLFGTFLCNNAKERGEKHTQERTCSVWSLLRAGNKAFKNLLYSSQSEAVL
  YPVCHVRNLMLWSAVYLPCPSPTTPVDDSCAPYPAPGTSPDDPPLSRLPKTRSYDNLTTACDNTVPLASR
  RCSDPSLNEKWQEHRRSLELSSLAGPGEDPLSADSLGKPTRVPGGAELSVAAGVAEGQMENILQEATKEE
  SGVEEPAHRAGIEIQEGKEDPLLEKESRRKTPEASAIGLHQDPELGDAALRSHLDMSWPLFSQGISEQQS
  GLSVLLSSLQVPPRGEDSLEVPVEQFRIEEIAEGREEAVLPIPVDAKVGYGTSQSCSLLPSQVPFETRGP
  NVDSSTDMLVEDKVKSVSGPQGHHRSCLVNSGKDRLPQTMEPSPSETSLVERPQVGSVVHRTSLGSTLSL
  TRSPCALPLAECKEGLVCNGAPETENRASEQPPGLSTLQMYPTPNGHCANGEAGRSKDSLSRQLSAMSCS
  SAHLHSRNLHHKWLHSHSGRPSATSSPDQPSRSHLDDDGMSVYTDTIQQRLRQIESGHQQEVETLKKQVQ
  ELKSRLESQYLTSSLHFNGDFGDEVTSIPDSESNLDQNCLSRCSTEIFSEASWEQVDKQDTEMTRWLPDH
  LAAHCYACDSAFWLASRKHHCRNCGNVFCSSCCNQKVPVPSQQLFEPSRVCKSCYSSLHPTSSSIDLELD
  KPIAATSN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021090
• ORF Size: 3594 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021090.4
• RefSeq Size: 5963 bp
• RefSeq ORF: 3597 bp
• Locus ID: 8897
• UniProt ID: Q13615
• Cytogenetics: 22q12.2
• Domains: FYVE, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: This gene encodes a member of the myotubularin dual specificity protein phosphatase gene family. The encoded protein is structurally similar to myotubularin but in addition contains a FYVE domain and an N-terminal PH-GRAM domain. The protein can self-associate and also form heteromers with another myotubularin related protein. The protein binds to phosphoinositide lipids through the PH-GRAM domain, and can hydrolyze phosphatidylinositol(3)-phosphate and phosphatidylinositol(3,5)-biphosphate in vitro. The encoded protein has been observed to have a perinuclear, possibly membrane-bound, distribution in cells, but it has also been found free in the cytoplasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]