MED1 (NM_004774) Human Tagged ORF Clone

CAT#: RG222118

MED1 (tGFP-tagged) - Human mediator complex subunit 1 (MED1)

Product Images

Circular map for RG222118

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MED1
• Synonyms: CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222118 representing NM_004774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCTCAGGGGGAAACCGAGGAGTCAGAAAAGCTGAGTAAGATGAGTTCTCTCCTGGAACGGCTCC
  ATGCAAAATTTAACCAAAATAGACCCTGGAGTGAAACCATTAAGCTTGTGCGTCAAGTCATGGAGAAGAG
  GGTTGTGATGAGTTCTGGAGGGCATCAACATTTGGTCAGCTGTTTGGAGACATTGCAGAAGGCTCTCAAA
  GTAACATCTTTACCAGCAATGACTGATCGTTTGGAGTCCATAGCAAGACAGAATGGACTGGGCTCTCATC
  TCAGTGCCAGTGGCACTGAATGTTACATCACGTCAGATATGTTCTATGTGGAAGTGCAGTTAGATCCTGC
  AGGACAGCTTTGTGATGTAAAAGTGGCTCACCATGGGGAGAATCCTGTGAGCTGTCCGGAGCTTGTACAG
  CAGCTAAGGGAAAAAAATTTTGATGAATTTTCTAAGCACCTTAAGGGCCTTGTTAATCTGTATAACCTTC
  CAGGGGACAACAAACTGAAGACTAAAATGTACTTGGCTCTCCAATCCTTAGAACAAGATCTTTCTAAAAT
  GGCAATTATGTACTGGAAAGCAACTAATGCTGGTCCCTTGGATAAGATTCTTCATGGAAGTGTTGGCTAT
  CTCACACCAAGGAGTGGGGGTCATTTAATGAACCTGAAGTACTATGTCTCTCCTTCTGACCTACTGGATG
  ACAAGACTGCATCTCCCATCATTTTGCATGAGAATAATGTTTCTCGATCTTTGGGCATGAATGCATCAGT
  GACAATTGAAGGAACATCTGCTGTGTACAAACTCCCAATTGCACCATTAATTATGGGGTCACATCCAGTT
  GACAATAAATGGACCCCTTCCTTCTCCTCAATCACCAGTGCCAACAGTGTTGATCTTCCTGCCTGTTTCT
  TCTTGAAATTTCCCCAGCCAATCCCAGTATCTAGAGCATTTGTTCAGAAACTGCAGAACTGCACAGGAAT
  TCCATTGTTTGAAACTCAACCAACTTATGCACCCCTGTATGAACTGATCACTCAGTTTGAGCTATCAAAG
  GACCCTGACCCCATACCTTTGAATCACAACATGAGATTTTATGCTGCTCTTCCTGGTCAGCAGCACTGCT
  ATTTCCTCAACAAGGATGCTCCTCTTCCAGATGGCCGAAGTCTACAGGGAACCCTTGTTAGCAAAATCAC
  CTTTCAGCACCCTGGCCGAGTTCCTCTTATCCTAAATCTGATCAGACACCAAGTGGCCTATAACACCCTC
  ATTGGAAGCTGTGTCAAAAGAACTATTCTGAAAGAAGATTCTCCTGGGCTTCTCCAATTTGAAGTGTGTC
  CTCTCTCAGAGTCTCGTTTCAGCGTATCTTTTCAGCACCCTGTGAATGACTCCCTGGTGTGTGTGGTAAT
  GGATGTGCAGGACTCAACACATGTGAGCTGTAAACTCTACAAAGGGCTGTCGGATGCACTGATCTGCACA
  GATGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATCCCTGTGACGATGAGGGCTATTCGGAGGA
  AAGCTGAAACCATTCAAGCCGACACCCCAGCACTGTCCCTCATTGCAGAGACAGTTGAAGACATGGTGAA
  AAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCACAGGCAACAACCCAATGAGTGGTACC
  ACTACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTGTTTAATATGAGCATGAGCATCAAAG
  ATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGTCTCAGAACCCAATTCTTACCAGTTT
  GTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCCGACCCCTCCTCATCACACGCCGCCA
  CCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTCATGAACCTTCTTAAAGATAATCCTG
  CCCAGGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGCAGAACTCCTCTTCCGGCTCACCCCG
  CATGGAAATATGCTCGGGGAGCAACAAGACCAAGAAAAAGAAGTCATCAAGATTACCACCTGAGAAACCA
  AAGCACCAGACTGAAGATGACTTTCAGAGGGAGCTATTTTCAATGGATGTTGACTCACAGAACCCTATCT
  TTGATGTCAACATGACAGCTGACACGCTGGATACGCCACACATCACTCCAGCTCCAAGCCAGTGTAGCAC
  TCCCCCAACAACTTACCCACAACCAGTACCTCACCCCCAACCCAGTATTCAAAGGATGGTCCGACTATCC
  AGTTCAGACAGCATTGGCCCAGATGTAACTGACATCCTTTCAGACATTGCAGAAGAAGCTTCTAAACTTC
  CCAGCACTAGTGATGATTGCCCAGCCATTGGCACCCCTCTTCGAGATTCTTCAAGCTCTGGGCATTCTCA
  GAGTACCCTGTTTGACTCTGATGTCTTTCAAACTAACAATAATGAAAATCCATACACTGATCCAGCTGAT
  CTTATTGCAGATGCTGCTGGAAGCCCCAGTAGTGACTCTCCTACCAATCATTTTTTTCATGATGGAGTAG
  ATTTCAATCCTGATTTATTGAACAGCCAGAGCCAAAGTGGTTTTGGAGAAGAATATTTTGATGAAAGCAG
  CCAAAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGCACTAAATACTTTGGGGGTGCCAATG
  CTTGGAGGTGATAATGGGGAGACCAAGTTTAAGGGCAATAACCAAGCCGACACAGTTGATTTCAGTATTA
  TTTCAGTAGCCGGCAAAGCTTTAGCTCCTGCAGATCTTATGGAGCATCACAGTGGTAGTCAGGGTCCTTT
  ACTGACCACTGGGGACTTAGGGAAAGAAAAGACTCAAAAGAGGGTAAAGGAAGGCAATGGCACCAGTAAT
  AGTACTCTCTCGGGGCCCGGATTAGACAGCAAACCAGGGAAGCGCAGTCGGACCCCTTCTAATGATGGGA
  AAAGCAAAGATAAGCCTCCAAAGCGGAAGAAGGCAGACACTGAGGGAAAGTCTCCATCTCATAGTTCTTC
  TAACAGACCTTTTACCCCACCTACCAGTACAGGTGGATCTAAATCGCCAGGCAGTGCAGGAAGATCTCAG
  ACTCCCCCAGGTGTTGCCACACCACCCATTCCCAAAATCACTATTCAGATTCCTAAGGGAACAGTGATGG
  TGGGCAAGCCTTCCTCTCACAGTCAGTATACCAGCAGTGGTTCTGTGTCTTCCTCAGGCAGCAAAAGCCA
  CCATAGCCATTCTTCCTCCTCTTCCTCATCTGCTTCCACCTCAGGGAAGATGAAAAGCAGTAAATCAGAA
  GGTTCATCAAGTTCCAAGTTAAGTAGCAGTATGTATTCTAGCCAGGGGTCTTCTGGATCTAGCCAGTCCA
  AAAATTCATCCCAGTCTGGGGGGAAGCCAGGCTCCTCTCCCATAACCAAGCATGGACTGAGCAGTGGCTC
  TAGCAGCACCAAGATGAAACCTCAAGGAAAGCCATCATCACTTATGAATCCTTCTTTAAGTAAACCAAAC
  ATATCCCCTTCTCATTCAAGGCCACCTGGAGGCTCTGACAAGCTTGCCTCTCCAATGAAGCCTGTTCCTG
  GAACTCCTCCATCCTCTAAAGCCAAGTCCCCTATCAGTTCAGGTTCTGGTGGTTCTCATATGTCTGGAAC
  TAGTTCAAGCTCTGGCATGAAGTCATCTTCAGGGTTAGGATCCTCAGGCTCGTTGTCCCAGAAAACTCCC
  CCATCATCTAATTCCTGTACGGCATCTTCCTCCTCCTTTTCCTCAAGTGGCTCTTCCATGTCATCCTCTC
  AGAACCAGCATGGGAGTTCTAAAGGAAAATCTCCCAGCAGAAACAAGAAGCCGTCCTTGACAGCTGTCAT
  AGATAAACTGAAGCATGGGGTTGTCACCAGTGGCCCTGGGGGTGAAGACCCACTGGACGGCCAGATGGGG
  GTGAGCACAAATTCTTCCAGCCATCCTATGTCCTCCAAACATAACATGTCAGGAGGAGAGTTTCAGGGCA
  AGCGTGAGAAAAGTGATAAAGACAAATCAAAGGTTTCCACCTCCGGGAGTTCAGTGGATTCTTCTAAGAA
  GACCTCAGAGTCAAAAAATGTGGGGAGCACAGGTGTGGCAAAAATTATCATCAGTAAGCATGATGGAGGC
  TCCCCTAGCATTAAAGCCAAAGTGACTTTGCAGAAACCTGGGGAAAGTAGTGGAGAAGGGCTTAGGCCTC
  AAATGGCTTCTTCTAAAAACTATGGCTCTCCACTCATCAGTGGTTCCACTCCAAAGCATGAGCGTGGCTC
  TCCCAGCCATAGTAAGTCACCAGCATATACCCCCCAGAATCTGGACAGTGAAAGTGAGTCAGGCTCCTCC
  ATAGCAGAGAAATCTTATCAGAATAGTCCCAGCTCAGACGATGGTATCCGACCACTTCCAGAATACAGCA
  CAGAGAAACATAAGAAGCACAAAAAGGAAAAGAAGAAAGTAAAAGACAAAGATAGGGACCGAGACCGGGA
  CAAAGACCGAGACAAGAAAAAATCTCATAGCATCAAGCCAGAGAGTTGGTCCAAATCACCCATCTCTTCA
  GACCAGTCCTTGTCTATGACAAGTAACACAATCTTATCTGCAGACAGACCCTCAAGGCTCAGCCCAGACT
  TTATGATTGGGGAGGAAGATGATGATCTTATGGATGTGGCCCTGATTGGGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222118 representing NM_004774
  Red=Cloning site Green=Tags(s)
  
  MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALK
  VTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQ
  QLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGY
  LTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPV
  DNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSK
  DPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTL
  IGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICT
  DDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGT
  TTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPP
  PVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKP
  KHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLS
  SSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPAD
  LIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPM
  LGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSN
  STLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQ
  TPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSE
  GSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPN
  ISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTP
  PSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMG
  VSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGG
  SPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSS
  IAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISS
  DQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004774
• ORF Size: 4743 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004774.4
• RefSeq Size: 5809 bp
• RefSeq ORF: 4746 bp
• Locus ID: 5469
• UniProt ID: Q15648
• Cytogenetics: 17q12
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Gene Summary: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008]