CDC42EP1 (NM_152243) Human Tagged ORF Clone

CAT#: RG222088

CDC42EP1 (tGFP-tagged) - Human CDC42 effector protein (Rho GTPase binding) 1 (CDC42EP1)

Product Images

Circular map for RG222088

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDC42EP1
• Synonyms: BORG5; CEP1; MSE55
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222088 representing NM_152243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGCCCCCAGGGGGGCAGAGGCGCCGCCACCATGAGCCTGGGCAAGCTCTCGCCTGTGGGCTGGG
  TGTCCAGTTCACAGGGAAAGAGGCGGCTGACTGCAGACATGATCAGCCACCCACTCGGGGACTTCCGCCA
  CACCATGCATGTGGGCCGTGGCGGGGATGTCTTCGGGGACACGTCCTTCCTCAGCAACCACGGTGGCAGC
  TCCGGGAGCACCCATCGCTCACCCCGCAGCTTCCTGGCCAAGAAGCTGCAGCTGGTGCGGAGGGTGGGGG
  CGCCCCCCCGGAGGATGGCATCTCCCCCTGCACCCTCCCCGGCTCCACCGGCCATCTCCCCCATCATCAA
  GAACGCCATCTCCCTGCCCCAGCTCAACCAGGCCGCCTACGACAGCCTCGTGGTTGGCAAGCTCAGCTTC
  GACAGCAGCCCCACCAGCTCCACGGACGGCCACTCCAGCTACGGCCTGGACTCTGGGTTCTGCACCATCT
  CCCGCCTGCCCCGCTCGGAAAAGCCGCATGACCGAGACCGGGATGGTTCCTTCCCCTCTGAGCCCGGGCT
  TCGCCGCTCTGACTCTCTCTTGTCCTTCCGCCTGGACCTCGACCTTGGGCCCTCACTCCTCAGCGAGCTG
  CTAGGGGTCATGAGCCTCCCAGAAGCCCCTGCAGCTGAGACTCCAGCCCCCGCTGCAAACCCCCCAGCCC
  CTACTGCAAACCCCACGGGTCCTGCTGCAAACCCCCCAGCCACTACTGCAAACCCCCCAGCGCCTGCTGC
  AAACCCCTCAGCACCTGCCGCAACCCCCACGGGTCCTGCTGCAAATCCCCCAGCCCCTGCCGCAAGCTCC
  ACACCCCATGGACACTGTCCCAATGGGGTAACAGCTGGGTTGGGCCCAGTGGCTGAGGTGAAGTCCAGCC
  CAGTGGGAGGGGGTCCCCGAGGACCTGCTGGCCCTGCCCTCGGCAGGCACTGGGGAGCAGGCTGGGATGG
  CGGCCACCACTACCCAGAGATGGATGCGCGGCAGGAGCGGGTGGAGGTGCTGCCCCAAGCCCGGGCCTCC
  TGGGAGAGCCTGGACGAAGAGTGGAGGGCGCCCCAGGCAGGCAGCAGGACCCCAGTGCCCAGCACAGTGC
  AAGCAAACACCTTTGAATTTGCGGATGCTGAGGAGGATGATGAGGTCAAGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222088 representing NM_152243
  Red=Cloning site Green=Tags(s)
  
  MPGPQGGRGAATMSLGKLSPVGWVSSSQGKRRLTADMISHPLGDFRHTMHVGRGGDVFGDTSFLSNHGGS
  SGSTHRSPRSFLAKKLQLVRRVGAPPRRMASPPAPSPAPPAISPIIKNAISLPQLNQAAYDSLVVGKLSF
  DSSPTSSTDGHSSYGLDSGFCTISRLPRSEKPHDRDRDGSFPSEPGLRRSDSLLSFRLDLDLGPSLLSEL
  LGVMSLPEAPAAETPAPAANPPAPTANPTGPAANPPATTANPPAPAANPSAPAATPTGPAANPPAPAASS
  TPHGHCPNGVTAGLGPVAEVKSSPVGGGPRGPAGPALGRHWGAGWDGGHHYPEMDARQERVEVLPQARAS
  WESLDEEWRAPQAGSRTPVPSTVQANTFEFADAEEDDEVKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152243
• ORF Size: 1173 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152243.3
• RefSeq Size: 2135 bp
• RefSeq ORF: 1176 bp
• Locus ID: 11135
• UniProt ID: Q00587
• Cytogenetics: 22q13.1
• Domains: PBD
• Gene Summary: CDC42 is a member of the Rho GTPase family that regulates multiple cellular activities, including actin polymerization. The protein encoded by this gene is a CDC42 binding protein that mediates actin cytoskeleton reorganization at the plasma membrane. This protein is secreted and is primarily found in bone marrow. [provided by RefSeq, Jul 2008]