LHCGR (NM_000233) Human Tagged ORF Clone

CAT#: RG222051

LHCGR (tGFP-tagged) - Human luteinizing hormone/choriogonadotropin receptor (LHCGR)

Product Images

Circular map for RG222051

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LHCGR
• Synonyms: HHG; LCGR; LGR2; LH/CG-R; LH/CGR; LHR; LHRHR; LSH-R; ULG5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222051 representing NM_000233
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCAGCGGTTCTCGGCGCTGCAGCTGCTGAAGCTGCTGCTGCTGCTGCAGCCGCCGCTGCCACGAG
  CGCTGCGCGAGGCGCTCTGCCCTGAGCCCTGCAACTGCGTGCCCGACGGCGCCCTGCGCTGCCCCGGCCC
  CACGGCCGGTCTCACTCGACTATCACTTGCCTACCTCCCTGTCAAAGTGATCCCATCTCAAGCTTTCAGA
  GGACTTAATGAGGTCATAAAAATTGAAATCTCTCAGATTGATTCCCTGGAAAGGATAGAAGCTAATGCCT
  TTGACAACCTCCTCAATTTGTCTGAAATACTGATCCAGAACACCAAAAATCTGAGATACATTGAGCCCGG
  AGCATTTATAAATCTTCCCGGATTAAAATACTTGAGCATCTGTAACACAGGCATCAGAAAGTTTCCAGAT
  GTTACGAAGGTCTTCTCCTCTGAATCAAATTTCATTCTGGAAATTTGTGATAACTTACACATAACCACCA
  TACCAGGAAATGCTTTTCAAGGGATGAATAATGAATCTGTAACACTCAAACTATATGGAAATGGATTTGA
  AGAAGTACAAAGTCATGCATTCAATGGGACGACACTGACTTCACTGGAGCTAAAGGAAAACGTACATCTG
  GAGAAGATGCACAATGGAGCCTTCCGTGGGGCCACAGGGCCGAAAACCTTGGATATTTCTTCCACCAAAT
  TGCAGGCCCTGCCGAGCTATGGCCTAGAGTCCATTCAGAGGCTAATTGCCACGTCATCCTATTCTCTAAA
  AAAATTGCCATCAAGAGAAACATTTGTCAATCTCCTGGAGGCCACGTTGACTTACCCCAGCCACTGCTGT
  GCTTTTAGAAACTTGCCAACAAAAGAACAGAATTTTTCACATTCCATTTCTGAAAACTTTTCCAAACAAT
  GTGAAAGCACAGTAAGGAAAGTGAGTAACAAAACACTTTATTCTTCCATGCTTGCTGAGAGTGAACTGAG
  TGGCTGGGACTATGAATATGGTTTCTGCTTACCCAAGACACCCCGATGTGCTCCTGAACCAGATGCTTTT
  AATCCCTGTGAAGACATTATGGGCTATGACTTCCTTAGGGTCCTGATTTGGCTGATTAATATTCTAGCCA
  TCATGGGAAACATGACTGTTCTTTTTGTTCTCCTGACAAGTCGTTACAAACTTACAGTGCCTCGTTTTCT
  CATGTGCAATCTCTCCTTTGCAGACTTTTGCATGGGGCTCTATCTGCTGCTCATAGCCTCAGTTGATTCC
  CAAACCAAGGGCCAGTACTATAACCATGCCATAGACTGGCAGACAGGGAGTGGGTGCAGCACTGCTGGCT
  TTTTCACTGTATTCGCAAGTGAACTTTCTGTCTACACCCTCACCGTCATCACTCTAGAAAGATGGCACAC
  CATCACCTATGCTATTCACCTGGACCAAAAGCTGCGATTAAGACATGCCATTCTGATTATGCTTGGAGGA
  TGGCTCTTTTCTTCTCTAATTGCTATGTTGCCCCTTGTCGGTGTCAGCAATTACATGAAGGTCAGTATTT
  GCTTCCCCATGGATGTGGAAACCACTCTCTCACAAGTCTATATATTAACCATCCTGATTCTCAATGTGGT
  GGCCTTCTTCATAATTTGTGCTTGCTACATTAAAATTTATTTTGCAGTTCGAAACCCAGAATTAATGGCT
  ACCAATAAAGATACAAAGATTGCTAAGAAAATGGCAATCCTCATCTTCACCGATTTCACCTGCATGGCAC
  CTATCTCTTTTTTTGCCATCTCAGCTGCCTTCAAAGTACCTCTTATCACAGTAACCAACTCTAAAGTTTT
  ACTGGTTCTTTTTTATCCCATCAATTCTTGTGCCAATCCATTTCTGTATGCAATATTCACTAAGACATTC
  CAAAGAGATTTCTTTCTTTTGCTGAGCAAATTTGGCTGCTGTAAACGTCGGGCTGAACTTTATAGAAGGA
  AAGATTTTTCAGCTTACACCTCCAACTGCAAAAATGGCTTCACTGGATCAAATAAGCCTTCTCAATCCAC
  CTTGAAGTTGTCCACATTGCACTGTCAAGGTACAGCTCTCCTAGACAAGACTCGCTACACAGAGTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222051 representing NM_000233
  Red=Cloning site Green=Tags(s)
  
  MKQRFSALQLLKLLLLLQPPLPRALREALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFR
  GLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPGLKYLSICNTGIRKFPD
  VTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHL
  EKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIATSSYSLKKLPSRETFVNLLEATLTYPSHCC
  AFRNLPTKEQNFSHSISENFSKQCESTVRKVSNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAF
  NPCEDIMGYDFLRVLIWLINILAIMGNMTVLFVLLTSRYKLTVPRFLMCNLSFADFCMGLYLLLIASVDS
  QTKGQYYNHAIDWQTGSGCSTAGFFTVFASELSVYTLTVITLERWHTITYAIHLDQKLRLRHAILIMLGG
  WLFSSLIAMLPLVGVSNYMKVSICFPMDVETTLSQVYILTILILNVVAFFIICACYIKIYFAVRNPELMA
  TNKDTKIAKKMAILIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVLFYPINSCANPFLYAIFTKTF
  QRDFFLLLSKFGCCKRRAELYRRKDFSAYTSNCKNGFTGSNKPSQSTLKLSTLHCQGTALLDKTRYTEC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000233
• ORF Size: 2097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000233.1, NP_000224.1
• RefSeq Size: 2995 bp
• RefSeq ORF: 2100 bp
• Locus ID: 3973
• UniProt ID: P22888
• Cytogenetics: 2p16.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes the receptor for both luteinizing hormone and choriogonadotropin. This receptor belongs to the G-protein coupled receptor 1 family, and its activity is mediated by G proteins which activate adenylate cyclase. Mutations in this gene result in disorders of male secondary sexual character development, including familial male precocious puberty, also known as testotoxicosis, hypogonadotropic hypogonadism, Leydig cell adenoma with precocious puberty, and male pseudohermaphtoditism with Leydig cell hypoplasia. [provided by RefSeq, Jul 2008]