TMC3 (NM_001080532) Human Tagged ORF Clone

CAT#: RG222033

TMC3 (tGFP-tagged) - Human transmembrane channel-like 3 (TMC3)

Product Images

Circular map for RG222033

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222033 representing NM_001080532
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACCTCGAAGGCATCCCAGCGCTATAGAGGCATCCGGAGAAATGCCAGCCAGTGCTACCTCTACC
  AGGAATCTCTGCTGCTCAGCAACTTGGATGACAGCTTTAGTGCTGATGAAACAGGGGACAGCAATGATCC
  GGAACAAATCTTCCAGAATATACAGTTCCAGAAAGATCTCATGGCAAACATTCGCTGCAGACCCTGGACT
  ATGGGACAGAAGCTGAGGGCATTGAGACAAGCGAAGAACATTGTGCTGAAGTTTGAAGGGAGGCTGACCA
  GGACCCGAGGCTACCAAGCAGCAGGTGCAGAGCTCTGGCGGAAATTTGCTCGTCTCGCCTGTAACTTTGT
  GGTCATCTTCATTCCCTGGGAAATGAGGATAAAGAAAATCGAGAGTCATTTTGGATCTGGCGTTGCCTCC
  TATTTCATATTCTTGAGATGGTTATTTGGAATTAATATTGTGCTCACCATTATGACAGGTGCTTTTATTG
  TCATTCCAGAGCTGATTGCAGGCCAGCCCTTTGGAAGCACAGCCAGGAAGACCATCCCCAAGGAGCAGGT
  TTCGTCTGCCCAAGACCTGGACACCGTCTGGTCTCTGGGGGGCTACCTCCAGTACTCTGTCCTCTTCTAC
  GGATATTACGGCAGGGAGAGGAAGATCGGGAGAGCTGGCTACCGGCTGCCCTTGGCGTATTTCCTAGTGG
  GGATGGCAGTGTTTGCTTACAGCTTCATCATTCTTTTAAAAAAGATGGCTAAGAACTCCCGCACGAGTCT
  TGCCAGTGCTTCCAATGAAAACTATACCTTCTGCTGGCGGGTGTTCTGTGCCTGGGATTACCTCATTGGA
  AACCCAGAGGCTGCAGAGAGCAAAACAGCTGCCATAGTGAACAGCATCAGGGAAGCTATATTGGAAGAAC
  AGGAGAAAAAGAAAAGCAAAAACCTGGCAGTGACCATCTGCCTGAGGATTATTGCCAACATCCTGGTGCT
  TCTCTCACTGGCTGGGAGCATTTATCTCATCTACTTTGTGGTGGACCGGTCCCAGAAGCTGGAGCAGTCG
  AAGAAGGAGCTGACACTTTGGGAAAAGAATGAGGTCAGTGTGGTGGTCTCCCTCGTCACCATGATAGCAC
  CATCAGCCTTTGACCTCATTGCTGCCTTAGAGATGTACCACCCCAGGACCACGCTGCGCTTCCAGCTTGC
  AAGGGTCCTTGTGCTGTATTTGGGAAATCTCTACAGCCTGATCATTGCTCTCCTGGACAAAGTTAACAGC
  ATGAGCATTGAGGAAATGGCTACCAAAAATAACACAAGTCATTGGATAGATTCCACCACCTTCTTTGCAA
  CCAGGACAGCCCCTGAAGAAGAGAAATGGTCCACATCTCGGCCTGGAATGGGGCTCAGGAGAAACAACAC
  ATGGGCCTTGGAAGAGACCAGCATTTCAGCTTATACCATGCCTCTTATAAAGGCCAACAAGACTAGCCTC
  CACACTCAGAGTCCACAAGACCAGTGCTGGGAGACATACGTTGGTCAGGAGATGCTGAAGCTCTCCATCA
  TTGACATGCTCTTCACCGTGGCGAGCATTCTGCTCATAGACTTCTTCCGAGGACTTTTCGTGCGGTACTT
  AAGTGACTACTGGTGTTGGGATCTGGAGAGCAAGTTTCCTGAATATGGAGAATTCAAAATAGCTGAGAAT
  GTGCTACATTTAGTCTACAACCAAGGAATGATTTGGATGGGGGCCTTCTTCTCCCCATGTCTCCCAGCGT
  TCAACGTTCTCAAACTCATTGGGCTCATGTACCTGAGAAGCTGGGCTGTGCTGACCTGCAATGTGCCCCA
  CCAGCAAGTATTTCGAGCCTCAAGATCCAACAACTTCTACTTGGCAATGCTCCTGTTTATGCTGTTTCTG
  TGCATGCTGCCAACCATTTTTGCTATTGTCCGATACAAGCCATCTCTAAACTGTGGGCCATTCAGTGGAC
  AAGAGAAAATTTATGACATTGTGTCAGAAACGATTGAGAAAGACTTTCCTGTGTGGTTTGGCTCCGTGGT
  TGGACACATCAGTAGCCCCGTGGTCATCCTGCCCGCAGTACTCCTGCTTTTCATGCTCATCTATTATCTC
  CAGAGCATCGCACGGTCTCTAAAGCTCAGCAACCACCAGCTCAAAATGCAGATCCAAAACGCAAGATCAG
  AGGATAAGAAAAAGGTTGCCCAGATGGTAGAAGCCCGAATCCAGACCCAGGAAGAAAGCACCAAGAAGCT
  TCCAAACGACAGTGATCTTACCAGCCAGCTGTCCTCAGCGCACTCGGGCACACCCCAAAACAACGGCAAC
  GTGGCCCATTTTGACTCAGGGAGCAGCAAGAGTGGCAGGATAGAGACAGTCGCACAGTCCATGCCCCAGA
  GCCCGAGGCCAGGGGACAGGGCTCCTAGCTCACCTCTCCCTGGGGTCCCCAAATCCAGGCTAGAGCATGA
  AACTAACAGGTATCTGCACGGTCTGTGTGCAAGCACCAGTGACCTTCACAGGAACAGATCGCGCACACCT
  ATGACATTTACAACGCACATCGAAGATGTACACTCAGAACCTCTTTTCCGAAAAGACTTTCAGCAAATCA
  ACCCTCCTCACCGTGGACCACAGGCCTCGACTTTGCTGGCACAGGGTCCCAGGCCCCACGCCCCCAGATA
  CTATGTCATTAATGAATGTGACTCTTACAAGAAAAAACATCTAAATGTCTGGCCAGAAAGACATTTCAAG
  ATAGATGCTTCAGGTGACATTGTGGAGCTGTACCCCAGGAACGTGCGACAATATGCATCCCGGGTCCCCC
  GCCAGCCTCCCTCCCCACAGCTGAGTGAAGAAGAGGAGGAGACGCCAAGCAGAGATTGGATCAAACGGTC
  TCTTCCCCCACGCTCCCTGATAGACCTCCGTCGGGCTCCTCATTTTTATATTGGGGAGAGGTCCGAAAGT
  CAGACCCGGGATCCCGAGCACCAGGGGAGGGTGCACTACAAGTCGTGGAATGAAGATTTTGAGGGTCACC
  TTGAGAGGCCAGCCTATGTGCCCAGAAAGCCACGATCCCGGAATTTCCAATACCCACAGCCCCCTCTGAA
  GCCCAGAGGGAAGCCCAGGTTCGAGCCATCCCTCACGGAATCTGACTCCGTGTCGGCAGCATCCAGCAGT
  GACCAGCAGAACAGCAGCGCTGACCAGTACCTGCAGGTCACCCACAGCCAGGGCAGGTTCCCGAGGTCCG
  TGGGCCAGCCCAGCAGGAGGAAGGCCAAGTCGGGGCAGGAGCTGACCGTGGATCTGGATGACTTGATTTG
  TTCAGATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222033 representing NM_001080532
  Red=Cloning site Green=Tags(s)
  
  MKTSKASQRYRGIRRNASQCYLYQESLLLSNLDDSFSADETGDSNDPEQIFQNIQFQKDLMANIRCRPWT
  MGQKLRALRQAKNIVLKFEGRLTRTRGYQAAGAELWRKFARLACNFVVIFIPWEMRIKKIESHFGSGVAS
  YFIFLRWLFGINIVLTIMTGAFIVIPELIAGQPFGSTARKTIPKEQVSSAQDLDTVWSLGGYLQYSVLFY
  GYYGRERKIGRAGYRLPLAYFLVGMAVFAYSFIILLKKMAKNSRTSLASASNENYTFCWRVFCAWDYLIG
  NPEAAESKTAAIVNSIREAILEEQEKKKSKNLAVTICLRIIANILVLLSLAGSIYLIYFVVDRSQKLEQS
  KKELTLWEKNEVSVVVSLVTMIAPSAFDLIAALEMYHPRTTLRFQLARVLVLYLGNLYSLIIALLDKVNS
  MSIEEMATKNNTSHWIDSTTFFATRTAPEEEKWSTSRPGMGLRRNNTWALEETSISAYTMPLIKANKTSL
  HTQSPQDQCWETYVGQEMLKLSIIDMLFTVASILLIDFFRGLFVRYLSDYWCWDLESKFPEYGEFKIAEN
  VLHLVYNQGMIWMGAFFSPCLPAFNVLKLIGLMYLRSWAVLTCNVPHQQVFRASRSNNFYLAMLLFMLFL
  CMLPTIFAIVRYKPSLNCGPFSGQEKIYDIVSETIEKDFPVWFGSVVGHISSPVVILPAVLLLFMLIYYL
  QSIARSLKLSNHQLKMQIQNARSEDKKKVAQMVEARIQTQEESTKKLPNDSDLTSQLSSAHSGTPQNNGN
  VAHFDSGSSKSGRIETVAQSMPQSPRPGDRAPSSPLPGVPKSRLEHETNRYLHGLCASTSDLHRNRSRTP
  MTFTTHIEDVHSEPLFRKDFQQINPPHRGPQASTLLAQGPRPHAPRYYVINECDSYKKKHLNVWPERHFK
  IDASGDIVELYPRNVRQYASRVPRQPPSPQLSEEEEETPSRDWIKRSLPPRSLIDLRRAPHFYIGERSES
  QTRDPEHQGRVHYKSWNEDFEGHLERPAYVPRKPRSRNFQYPQPPLKPRGKPRFEPSLTESDSVSAASSS
  DQQNSSADQYLQVTHSQGRFPRSVGQPSRRKAKSGQELTVDLDDLICSDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080532
• ORF Size: 3300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080532.3
• RefSeq Size: 3303 bp
• RefSeq ORF: 3303 bp
• Locus ID: 342125
• UniProt ID: Q7Z5M5
• Cytogenetics: 15q25.1
• Protein Families: Transmembrane
• Gene Summary: Probable ion channel.[UniProtKB/Swiss-Prot Function]