TMEM39B (NM_018056) Human Tagged ORF Clone

CAT#: RG221912

TMEM39B (tGFP-tagged) - Human transmembrane protein 39B (TMEM39B)

Product Images

Circular map for RG221912

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TMEM39B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221912 representing NM_018056
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGACGAAGAGGTCCCAACAGGACATCTTACTGTCGAAATCCGCTCTGTGAGCCGGGATCCTCGG
  GGGGCTCTAGTGGAAGCCACACTTCCAGTGCATCGGTGACCAGTGTTCGTTCCCGCACCAGGAGCAGTTC
  TGGAACAGGCCTCTCCAGCCCTCCTCTGGCCACCCAAACTGTTGTGCCTCTACAGCACTGCAAGATCCCC
  GAGCTGCCAGTCCAGGCCAGCATTCTGTTTGAGTTGCAGCTCTTCTTCTGCCAGCTCATAGCACTCTTCG
  TCCACTACATCAACATCTACAAGACAGTGTGGTGGTATCCACCTTCCCACCCACCCTCCCACACCTCCCT
  GAACTTCCATCTGATCGACTTCAACTTGCTGATGGTGACCACCATCGTTCTGGGCCGCCGCTTCATTGGG
  TCCATCGTGAAGGAGGCCTCTCAGAGGGGGAAGGTCTCCCTCTTTCGCTCCATCCTGCTGTTCCTCACTC
  GCTTCACCGTTCTCACGGCAACAGGCTGGAGTCTGTGCCGATCCCTCATCCACCTCTTCAGGACCTACTC
  CTTCCTGAACCTCCTGTTCCTCTGCTATCCGTTTGGGATGTACATTCCGTTCCTGCAGCTGAATTGCGAC
  CTCCGCAAGACAAGCCTCTTCAACCACATGGCCTCCATGGGGCCCCGGGAGGCGGTCAGTGGCCTGGCAA
  AGAGCCGGGACTACCTCCTGACACTGCGGGAGACGTGGAAGCAGCACACAAGACAGCTGTATGGCCCGGA
  CGCCATGCCCACCCATGCCTGCTGCCTGTCACCCAGCCTCATCCGCAGTGAGGTGGAGTTCCTCAAGATG
  GACTTCAACTGGCGCATGAAGGAAGTGCTCGTCAGCTCCATGCTGAGCGCCTACTATGTGGCCTTTGTGC
  CTGTCTGGTTCGTGAAGAACACACATTACTATGACAAGCGCTGGTCCTGTGAACTCTTCCTGCTGGTGTC
  CATCAGCACCTCCGTGATCCTCATGCAGCACCTGCTGCCTGCCAGCTACTGTGACCTGCTGCACAAGGCC
  GCCGCCCATCTGGGCTGTTGGCAGAAGGTGGACCCAGCGCTGTGCTCCAACGTGCTGCAGCACCCGTGGA
  CTGAAGAATGCATGTGGCCGCAGGGCGTGCTGGTGAAGCACAGCAAGAACGTCTACAAAGCCGTAGGCCA
  CTACAACGTGGCTATCCCCTCTGACGTCTCCCACTTCCGCTTCCATTTCTTTTTCAGCAAACCCCTGCGG
  ATCCTCAACATCCTCCTGCTGCTGGAGGGCGCTGTCATTGTCTATCAGCTGTACTCCCTAATGTCCTCTG
  AAAAGTGGCACCAGACCATCTCGCTGGCCCTCATCCTCTTCAGCAACTACTATGCCTTCTTCAAGCTGCT
  CCGGGACCGCTTGGTATTGGGCAAGGCCTACTCATACTCTGCTAGCCCCCAGAGAGACCTGGACCACCGT
  TTCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221912 representing NM_018056
  Red=Cloning site Green=Tags(s)
  
  MGGRRGPNRTSYCRNPLCEPGSSGGSSGSHTSSASVTSVRSRTRSSSGTGLSSPPLATQTVVPLQHCKIP
  ELPVQASILFELQLFFCQLIALFVHYINIYKTVWWYPPSHPPSHTSLNFHLIDFNLLMVTTIVLGRRFIG
  SIVKEASQRGKVSLFRSILLFLTRFTVLTATGWSLCRSLIHLFRTYSFLNLLFLCYPFGMYIPFLQLNCD
  LRKTSLFNHMASMGPREAVSGLAKSRDYLLTLRETWKQHTRQLYGPDAMPTHACCLSPSLIRSEVEFLKM
  DFNWRMKEVLVSSMLSAYYVAFVPVWFVKNTHYYDKRWSCELFLLVSISTSVILMQHLLPASYCDLLHKA
  AAHLGCWQKVDPALCSNVLQHPWTEECMWPQGVLVKHSKNVYKAVGHYNVAIPSDVSHFRFHFFFSKPLR
  ILNILLLLEGAVIVYQLYSLMSSEKWHQTISLALILFSNYYAFFKLLRDRLVLGKAYSYSASPQRDLDHR
  FS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018056
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018056.4
• RefSeq Size: 1834 bp
• RefSeq ORF: 1479 bp
• Locus ID: 55116
• UniProt ID: Q9GZU3
• Cytogenetics: 1p35.2
• Protein Families: Transmembrane