FACL4 (ACSL4) (NM_022977) Human Tagged ORF Clone

CAT#: RG221413

ACSL4 (tGFP-tagged) - Human acyl-CoA synthetase long-chain family member 4 (ACSL4), transcript variant 2

Product Images

Circular map for RG221413

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FACL4
• Synonyms: ACS4; FACL4; LACS4; MRX63; MRX68
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221413 representing NM_022977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTTAAGCTAAATGTGCTCACCATTATTTTGCTGCCTGTCCACTTGTTAATAACAATATACAGTG
  CCCTTATATTTATTCCATGGTATTTTCTTACCAATGCCAAGAAGAAAAACGCTATGGCAAAGAGAATAAA
  AGCTAAGCCCACTTCAGACAAACCTGGAAGTCCATATCGCTCTGTCACACACTTCGACTCACTAGCTGTA
  ATAGACATCCCTGGAGCAGATACTCTGGATAAATTATTTGACCATGCTGTATCCAAGTTTGGGAAGAAGG
  ACAGCCTTGGGACCAGGGAAATCCTAAGTGAAGAAAATGAAATGCAGCCAAATGGAAAAGTTTTTAAGAA
  GTTAATTCTTGGGAATTATAAATGGATGAACTATCTTGAAGTGAATCGCAGAGTGAATAACTTTGGTAGT
  GGACTCACTGCACTGGGACTAAAACCAAAGAACACCATTGCCATCTTCTGTGAGACCAGGGCCGAATGGA
  TGATTGCAGCACAGACCTGCTTTAAGTACAACTTTCCTCTTGTGACTTTATATGCCACACTTGGCAAAGA
  AGCAGTAGTTCATGGGCTAAATGAATCTGAGGCTTCCTATCTGATTACCAGTGTTGAACTTCTGGAAAGT
  AAACTTAAGACTGCATTGTTAGATATCAGTTGTGTTAAACATATCATTTATGTGGACAATAAGGCTATCA
  ATAAAGCAGAGTACCCTGAAGGATTTGAGATTCACAGCATGCAATCAGTAGAAGAGTTGGGATCTAACCC
  AGAAAACTTGGGCATTCCTCCAAGTAGACCAACGCCTTCAGACATGGCCATTGTTATGTATACTAGTGGT
  TCTACTGGCCGACCTAAGGGAGTGATGATGCATCATAGCAATTTGATAGCTGGAATGACAGGCCAGTGTG
  AAAGAATACCTGGACTGGGACCGAAGGACACATATATTGGCTACTTGCCTTTGGCTCATGTGCTAGAACT
  GACAGCAGAGATATCTTGCTTTACCTATGGCTGCAGGATTGGATATTCTTCTCCGCTTACACTCTCTGAC
  CAGTCCAGCAAAATTAAAAAAGGAAGCAAAGGAGACTGTACTGTACTGAAGCCCACACTTATGGCTGCTG
  TTCCGGAAATCATGGATAGAATTTATAAGAATGTTATGAGCAAAGTCCAAGAGATGAATTATATTCAGAA
  AACTCTGTTCAAGATAGGGTATGATTACAAATTGGAACAGATCAAAAAGGGATATGATGCACCTCTTTGC
  AATCTGTTACTGTTTAAAAAGGTCAAGGCCCTGCTGGGAGGGAATGTCCGCATGATGCTGTCTGGAGGGG
  CCCCGCTATCTCCTCAGACACACCGATTCATGAATGTCTGCTTCTGCTGCCCAATTGGCCAGGGTTATGG
  ACTGACAGAATCATGTGGTGCTGGGACAGTTACTGAAGTAACTGACTATACTACTGGCAGAGTTGGAGCA
  CCTCTTATTTGCTGTGAAATTAAGCTAAAAGACTGGCAAGAAGGCGGTTATACAATTAATGACAAGCCAA
  ACCCCAGAGGTGAAATCGTAATTGGTGGACAGAACATCTCCATGGGATATTTTAAAAATGAAGAGAAAAC
  AGCAGAAGATTATTCTGTGGATGAAAATGGACAAAGGTGGTTTTGCACTGGTGATATTGGAGAATTCCAT
  CCCGATGGATGTTTACAGATTATAGATCGTAAGAAAGATCTAGTGAAGTTACAAGCAGGAGAGTATGTAT
  CTCTTGGGAAAGTAGAAGCTGCACTGAAGAATTGTCCACTTATTGACAACATCTGTGCTTTTGCCAAAAG
  TGATCAGTCCTATGTGATCAGTTTTGTGGTTCCTAACCAGAAAAGGTTGACACTTTTGGCACAACAGAAA
  GGGGTAGAAGGAACTTGGGTTGATATCTGCAATAATCCTGCTATGGAAGCTGAAATACTGAAAGAAATTC
  GAGAAGCTGCAAATGCCATGAAATTGGAGCGATTTGAAATTCCAATCAAGGTTCGATTAAGCCCAGAGCC
  ATGGACCCCTGAAACTGGTTTGGTAACTGATGCTTTCAAACTGAAAAGGAAGGAGCTGAGGAACCATTAC
  CTCAAAGACATTGAACGAATGTATGGGGGCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221413 representing NM_022977
  Red=Cloning site Green=Tags(s)
  
  MKLKLNVLTIILLPVHLLITIYSALIFIPWYFLTNAKKKNAMAKRIKAKPTSDKPGSPYRSVTHFDSLAV
  IDIPGADTLDKLFDHAVSKFGKKDSLGTREILSEENEMQPNGKVFKKLILGNYKWMNYLEVNRRVNNFGS
  GLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLES
  KLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSG
  STGRPKGVMMHHSNLIAGMTGQCERIPGLGPKDTYIGYLPLAHVLELTAEISCFTYGCRIGYSSPLTLSD
  QSSKIKKGSKGDCTVLKPTLMAAVPEIMDRIYKNVMSKVQEMNYIQKTLFKIGYDYKLEQIKKGYDAPLC
  NLLLFKKVKALLGGNVRMMLSGGAPLSPQTHRFMNVCFCCPIGQGYGLTESCGAGTVTEVTDYTTGRVGA
  PLICCEIKLKDWQEGGYTINDKPNPRGEIVIGGQNISMGYFKNEEKTAEDYSVDENGQRWFCTGDIGEFH
  PDGCLQIIDRKKDLVKLQAGEYVSLGKVEAALKNCPLIDNICAFAKSDQSYVISFVVPNQKRLTLLAQQK
  GVEGTWVDICNNPAMEAEILKEIREAANAMKLERFEIPIKVRLSPEPWTPETGLVTDAFKLKRKELRNHY
  LKDIERMYGGK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022977
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022977.2
• RefSeq Size: 5356 bp
• RefSeq ORF: 2136 bp
• Locus ID: 2182
• UniProt ID: O60488
• Cytogenetics: Xq23
• Domains: AMP-binding
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• Gene Summary: The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants. [provided by RefSeq, Jan 2016]