SENP7 (NM_020654) Human Tagged ORF Clone

CAT#: RG221411

SENP7 (tGFP-tagged) - Human SUMO1/sentrin specific peptidase 7 (SENP7), transcript variant 1

Product Images

Circular map for RG221411

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SENP7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221411 representing NM_020654
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGAGAAAGCTCGGGCGACGGCCATCTTCATCCGAAATCATCACAGAAGGAAAAAGGAAAAAGT
  CATCTTCTGATTTATCGGAGATAAGAAAGATGTTAAATGCAAAACCAGAGGATGTCCATGTTCAATCACC
  ACTGTCCAAATTCAGAAGCTCAGAACGCTGGACTCTCCCTTTGCAGTGGGAAAGAAGCCTAAGGAATAAA
  GTCATCTCTCTAGACCATAAAAATCAAAAACATATCCGAGGGTGTCCTGTTACTTCCAAGTCATCACCAG
  AAAGGCAACTCAAAGTTATGTTGACGAATGTCCTATGGACGGATTTAGGACGAAAATTCAGAAAGACCCT
  ACCTAGAAACGATGCTAATTTATGTGATGCCAACAAGGTGCAATCAGACTCATTGCCTTCGACATCTGTT
  GACAGCCTAGAGACATGTCAAAAATTAGAACCTCTTCGCCAAAGCCTTAATTTATCTGAAAGGATACCCA
  GAGTTATATTGACGAATGTCCTGGGAACGGAGTTAGGAAGAAAATACATAAGGACCCCACCTGTAACTGA
  GGGAAGTTTGAGTGATACAGACAACTTGCAATCAGAGCAACTTTCTTCATCATCTGATGGCAGCCTAGAA
  TCTTATCAAAATCTAAACCCTCACAAGAGCTGTTATTTATCTGAAAGGGGCTCACAACGAAGTAAGACAG
  TAGATGACAATTCTGCAAAGCAGACTGCGCACAATAAAGAAAAACGAAGAAAGGATGATGGCATTTCTCT
  TTTAATATCTGATACTCAGCCTGAAGACCTTAACAGTGGAAGTAGAGGTTGTGATCATCTCGAACAGGAA
  AGCAGAAACAAGGATGTTAAATATTCTGATTCAAAAGTGGAACTCACTCTGATTTCCAGGAAGACAAAGA
  GAAGGCTTAGAAATAATTTACCTGATTCTCAATATTGTACTTCTTTGGATAAGTCAACAGAACAGACAAA
  AAAACAAGAAGATGACTCAACAATATCCACTGAGTTTGAAAAGCCAAGTGAAAACTATCATCAGGATCCA
  AAACTGCCTGAAGAAATTACAACTAAACCTACAAAAAGTGATTTTACTAAACTATCCTCACTTAACAGTC
  AGGAGTTGACTTTGAGTAATGCCACCAAAAGTGCCTCTGCCGGTTCAACCACTGAAACCGTTGAGAACTC
  TAATTCCATTGATATTGTGGGGATTTCTTCCCTGGTTGAGAAGGATGAGAATGAGTTGAATACCATAGAA
  AAGCCTATTCTAAGAGGACATAATGAAGGGAACCAATCACTGATCTCAGCTGAACCAATTGTTGTTTCCA
  GTGATGAAGAAGGACCTGTTGAACATAAAAGTTCAGAAATTCTTAAGTTACAATCTAAGCAAGACCGTGA
  GACAACTAATGAAAATGAGAGTACTTCTGAATCAGCATTGTTAGAACTACCATTGATTACATGTGAATCT
  GTACAGATGTCATCTGAATTATGCCCATATAATCCTGTCATGGAGAACATTTCCAGTATTATGCCTAGTA
  ATGAGATGGATCTACAACTGGATTTTATATTTACTTCTGTTTATATTGGTAAAATAAAAGGAGCTTCTAA
  AGGTTGTGTTACAATCACAAAAAAATATATTAAGATCCCATTTCAAGTGTCCCTGAATGAGATTTCATTG
  CTAGTGGATACCACACATTTAAAGCGGTTTGGGTTATGGAAAAGTAAGGATGATAATCACAGTAAAAGGA
  GTCATGCTATTCTTTTCTTCTGGGTCTCTTCAGATTATCTTCAAGAGATTCAGACCCAATTAGAACACTC
  TGTATTAAGCCAGCAATCAAAATCTAGTGAATTCATTTTCCTTGAACTACACAATCCTGTTTCACAAAGA
  GAAGAATTGAAGCTGAAAGATATTATGACGGAAATAAGTATAATCAGTGGAGAATTAGAGCTTTCTTACC
  CGTTGTCTTGGGTTCAGGCATTTCCTTTGTTTCAGAACCTCTCTTCAAAAGAAAGTTCTTTTATTCATTA
  TTACTGTGTTTCAACTTGTTCTTTCCCTGCTGGTGTTGCTGTTGCTGAAGAAATGAAGCTGAAATCAGTA
  TCTCAGCCCTCAAACACAGATGCGGCCAAGCCTACTTACACCTTCCTGCAGAAGCAAAGTAGCGGTTGCT
  ACTCCCTTTCTATTACATCTAATCCAGATGAAGAATGGCGAGAAGTCAGGCACACTGGACTTGTTCAGAA
  GTTGATTGTATATCCTCCACCACCTACTAAGGGGGGATTAGGAGTAACTAATGAAGATCTGGAGTGTTTA
  GAAGAAGGAGAGTTTCTTAATGATGTAATCATTGATTTTTACCTTAAGTATCTTATATTGGAGAAGGCAT
  CAGATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCTTTTTCTATAAATGCTTGACAAGAAAGGAAAA
  TAATTTAACAGAAGATAATCCAAATCTTTCAATGGCACAGAGAAGACATAAAAGAGTAAGAACATGGACT
  CGTCACATAAACATTTTTAATAAAGATTACATCTTTGTACCTGTAAATGAGTCGTCTCACTGGTATCTCG
  CAGTCATTTGTTTTCCATGGTTAGAAGAAGCTGTGTATGAAGATTTTCCACAAACTGTATCCCAGCAGTC
  CCAGGCTCAGCAGTCCCAAAATGACAACAAAACAATAGATAATGATCTACGTACTACTTCGACACTGTCT
  TTGAGTGCAGAGGATTCCCAAAGTACCGAGTCGAATATGTCAGTACCAAAGAAAATGTGTAAAAGGCCAT
  GTATTCTTATACTAGACTCCTTGAAAGCTGCTTCTGTACAAAACACAGTTCAGAATTTACGAGAGTATTT
  AGAGGTAGAGTGGGAAGTTAAACTAAAAACTCATCGTCAATTCAGCAAAACAAACATGGTGGATCTATGC
  CCTAAAGTTCCTAAACAGGACAATAGCAGTGATTGTGGAGTATATTTATTGCAGTATGTGGAAAGCTTCT
  TCAAGGATCCTATTGTTAACTTTGAACTTCCAATTCATTTGGAGAAGTGGTTTCCTCGTCATGTAATAAA
  GACCAAACGGGAAGATATTCGAGAGCTCATCTTGAAACTTCATTTACAGCAACAGAAGGGCAGCAGTAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221411 representing NM_020654
  Red=Cloning site Green=Tags(s)
  
  MDKRKLGRRPSSSEIITEGKRKKSSSDLSEIRKMLNAKPEDVHVQSPLSKFRSSERWTLPLQWERSLRNK
  VISLDHKNQKHIRGCPVTSKSSPERQLKVMLTNVLWTDLGRKFRKTLPRNDANLCDANKVQSDSLPSTSV
  DSLETCQKLEPLRQSLNLSERIPRVILTNVLGTELGRKYIRTPPVTEGSLSDTDNLQSEQLSSSSDGSLE
  SYQNLNPHKSCYLSERGSQRSKTVDDNSAKQTAHNKEKRRKDDGISLLISDTQPEDLNSGSRGCDHLEQE
  SRNKDVKYSDSKVELTLISRKTKRRLRNNLPDSQYCTSLDKSTEQTKKQEDDSTISTEFEKPSENYHQDP
  KLPEEITTKPTKSDFTKLSSLNSQELTLSNATKSASAGSTTETVENSNSIDIVGISSLVEKDENELNTIE
  KPILRGHNEGNQSLISAEPIVVSSDEEGPVEHKSSEILKLQSKQDRETTNENESTSESALLELPLITCES
  VQMSSELCPYNPVMENISSIMPSNEMDLQLDFIFTSVYIGKIKGASKGCVTITKKYIKIPFQVSLNEISL
  LVDTTHLKRFGLWKSKDDNHSKRSHAILFFWVSSDYLQEIQTQLEHSVLSQQSKSSEFIFLELHNPVSQR
  EELKLKDIMTEISIISGELELSYPLSWVQAFPLFQNLSSKESSFIHYYCVSTCSFPAGVAVAEEMKLKSV
  SQPSNTDAAKPTYTFLQKQSSGCYSLSITSNPDEEWREVRHTGLVQKLIVYPPPPTKGGLGVTNEDLECL
  EEGEFLNDVIIDFYLKYLILEKASDELVERSHIFSSFFYKCLTRKENNLTEDNPNLSMAQRRHKRVRTWT
  RHINIFNKDYIFVPVNESSHWYLAVICFPWLEEAVYEDFPQTVSQQSQAQQSQNDNKTIDNDLRTTSTLS
  LSAEDSQSTESNMSVPKKMCKRPCILILDSLKAASVQNTVQNLREYLEVEWEVKLKTHRQFSKTNMVDLC
  PKVPKQDNSSDCGVYLLQYVESFFKDPIVNFELPIHLEKWFPRHVIKTKREDIRELILKLHLQQQKGSSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020654
• ORF Size: 3150 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020654.3, NP_065705.3
• RefSeq Size: 4934 bp
• RefSeq ORF: 3153 bp
• Locus ID: 57337
• UniProt ID: Q9BQF6
• Cytogenetics: 3q12.3
• Domains: Peptidase_C48
• Protein Families: Druggable Genome, Protease
• Gene Summary: The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for many cellular processes. SUMO-specific proteases, such as SENP7, process SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009]