LCHN (KIAA1147) (NM_001080392) Human Tagged ORF Clone

CAT#: RG221277

KIAA1147 (tGFP-tagged) - Human KIAA1147 (KIAA1147)

Product Images

Circular map for RG221277

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LCHN
• Synonyms: KIAA1147; LCHN; PRO2561
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221277 representing NM_001080392
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGCAGGGAGACGCGGCGCCGCTGCTGCGCTGGGCCGAGGGCCCCGCCGTCTCCCTGCCGCAGG
  CCCCGCAGCCGCAGGCGGGAGGCTGGGGCCGGGGCGGCGGCGGGGGCGCCCGGCCGGCCGCGGAGCCGCC
  CCGGAGGCGGGAGCCCGAGGAGCCGGCCGCCCCGGAGGTGCTGCTGCAGCCCGGGCGCCTGGAGCTGGGC
  GACGTGGAGGAGGACCAGGTGGTGGCCGTGTTCGTGGTCACCTTCGACCCCCGCTCGGGAAACATGGTAG
  AATGGTGCTTACCTCAAGATATTGACCTTGAAGGTGTTGAGTTCAAGTCTATGGCCAGTGGGTCCCATAA
  AATCCAATCTGATTTCATCTATTTCCGAAAGGGGCCCTTCTTCGGCCTGGCCTGCTTTGCCAACATGCCC
  GTGGAGAGCGAGCTGGAACGTGGCGCGCGGATGAAGTCTGTGGGCATCCTCTCTCCCTCCTACACACTGC
  TTTACCGCTACATGCACTTCTTGGAGAACCAGGTTCGGCACCAGTTGGAGATGCCAGGACATTACTCTCA
  TCTGGCTGCCTTCTATGAGGACAAAAAGGGGGTGCTCCATGCTGGTCCCGGCAGAGGCAGCAGCCTGCCC
  CCTGTCTACTGGCTGCCTTCCATCCACCGATACATGTACCCTGAGATGAAGATCACACACCCAGCTGGCT
  GCATGTCTCAGTTTATAAAGTTCTTTGGAGAACAGATCCTCATCCTCTGGAAATTTGCCTTACTTCGAAA
  GCGCATTTTGATATTTTCTCCCCCACCTGTGGGCGTGGTGTGCTATAGAGTGTACTGCTGCTGCTGCTTG
  GCCAACGTTTCACTGCCTGGCATCGGGGGCACCATTCCTGAGTCCAAACCTTTCTTCTACGTGAACGTGG
  CTGACATCGAGAGCCTGGAGGTAGAGGTGTCCTATGTGGCCTGCACCACAGAGAAGATATTCGAGGAGAA
  GCGGGAGCTGTATGACGTCTACGTGGATAACCAGAATGTGAAGACACACCACGACCACCTGCAGCCGCTG
  CTGAAGATCAACAGTGCTGACAGGGAGAAGTACCGCCGGCTCAACGAGCAGAGGCAGATGCTGTTGTACT
  CCCAGGAAGTAGAAGAAGACTACAACCCTTGTGAAGAGGACCTCTTCGTGCTGTTTTTCCTAGAACAAAA
  CAACCGGATATTTCAGACTTTGTTGGAGGTGTCTGCCAGTCAAGACAAAACTCTGACAGCAGAGCATGCC
  CGGGGCATGGGCCTAGACCCCCAAGGAGACCGGAGCTTTCTCCTGGACCTGCTGGAGGCCTATGGCATTG
  ATGTCATGCTGGTTATCGACAACCCCTGTTGCCCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221277 representing NM_001080392
  Red=Cloning site Green=Tags(s)
  
  MVEQGDAAPLLRWAEGPAVSLPQAPQPQAGGWGRGGGGGARPAAEPPRRREPEEPAAPEVLLQPGRLELG
  DVEEDQVVAVFVVTFDPRSGNMVEWCLPQDIDLEGVEFKSMASGSHKIQSDFIYFRKGPFFGLACFANMP
  VESELERGARMKSVGILSPSYTLLYRYMHFLENQVRHQLEMPGHYSHLAAFYEDKKGVLHAGPGRGSSLP
  PVYWLPSIHRYMYPEMKITHPAGCMSQFIKFFGEQILILWKFALLRKRILIFSPPPVGVVCYRVYCCCCL
  ANVSLPGIGGTIPESKPFFYVNVADIESLEVEVSYVACTTEKIFEEKRELYDVYVDNQNVKTHHDHLQPL
  LKINSADREKYRRLNEQRQMLLYSQEVEEDYNPCEEDLFVLFFLEQNNRIFQTLLEVSASQDKTLTAEHA
  RGMGLDPQGDRSFLLDLLEAYGIDVMLVIDNPCCP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080392
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080392.2
• RefSeq Size: 7296 bp
• RefSeq ORF: 1368 bp
• Locus ID: 57189
• UniProt ID: A4D1U4
• Cytogenetics: 7q34
• Gene Summary: May play a role in neuritogenesis, as well as in neuronal recovery and/or restructuring in the hippocampus following transient cerebral ischemia.[UniProtKB/Swiss-Prot Function]