HS6ST3 (NM_153456) Human Tagged ORF Clone

CAT#: RG221187

HS6ST3 (tGFP-tagged) - Human heparan sulfate 6-O-sulfotransferase 3 (HS6ST3)

Product Images

Circular map for RG221187

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HS6ST3
• Synonyms: HS6ST-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221187 representing NM_153456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAAGGTTCAACAAGTGGCTGCTGACGCCGGTGCTCACTCTCCTCTTCGTGGTCATCATGTACC
  AGTACGTGTCCCCCTCCTGCACCAGCTCCTGCACCAACTTCGGGGAGCAGCCCCGCGCGGGGGAGGCCGG
  CCCGCCCGCCGTCCCGGGTCCCGCCCGCCGGGCTCAGGCGCCGCCGGAGGAGTGGGAGCGGCGGCCCCAG
  TTGCCCCCGCCGCCCCGGGGGCCCCCCGAGGGACCTCGGGGGGCCGCGGCGCCGGAGGAGGAGGACGAGG
  AGCCCGGAGACCCCCGGGAGGGGGAGGAAGAGGAGGAGGAAGACGAGCCGGACCCCGAGGCCCCGGAAAA
  CGGCTCCCTGCCCCGATTCGTGCCGCGCTTCAACTTCAGCCTGAAGGACCTGACCCGCTTCGTGGATTTC
  AACATCAAAGGGCGCGACGTGATCGTGTTCCTCCACATCCAGAAGACGGGGGGCACCACTTTCGGCCGGC
  ACCTGGTGAAGAACATCCGGCTGGAGCAGCCTTGTAGCTGCAAAGCGGGTCAGAAGAAGTGCACCTGCCA
  CCGGCCTGGCAAGAAGGAGACGTGGCTCTTCTCCCGCTTCTCCACCGGCTGGAGCTGCGGGCTGCACGCC
  GACTGGACGGAGCTCACCAACTGCGTGCCGGCCATCATGGAGAAGAAGGACTGTCCCCGCAACCACAGCC
  ACACCAGGAATTTCTATTACATCACAATGTTACGGGATCCAGTGTCACGTTACCTGAGCGAGTGGAAACA
  TGTCCAGAGAGGGGCCACTTGGAAAACCTCTCTTCATATGTGTGATGGAAGAAGCCCCACCCCAGATGAG
  CTGCCTACCTGCTACCCTGGGGATGACTGGTCTGGGGTCAGCTTGCGGGAGTTTATGGATTGCACCTACA
  ACCTGGCTAACAATCGCCAGGTGCGCATGCTGGCTGACCTCAGCCTGGTGGGCTGCTATAACTTGACTTT
  CATGAACGAGAGTGAAAGAAACACCATCCTGTTGCAGAGTGCAAAGAACAACCTGAAGAACATGGCCTTC
  TTTGGGCTCACTGAGTTCCAGAGGAAGACACAGTTTCTCTTTGAGAGAACATTCAACCTCAAGTTCATCT
  CCCCCTTCACACAGTTCAACATCACGCGGGCTTCTAACGTGGAGATCAACGAGGGTGCCCGCCAACGCAT
  TGAGGATCTAAACTTCCTGGACATGCAGCTTTACGAGTATGCAAAAGATCTCTTCCAGCAGCGCTACCAC
  CACACCAAGCAGCTAGAGCACCAGAGGGACCGCCAGAAGCGGCGGGAGGAGCGGAGGCTGCAGCGAGAGC
  ACAGGGACCACCAGTGGCCCAAAGAAGATGGGGCTGCAGAAGGGACTGTCACCGAGGACTACAACAGCCA
  GGTGGTGAGATGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221187 representing NM_153456
  Red=Cloning site Green=Tags(s)
  
  MDERFNKWLLTPVLTLLFVVIMYQYVSPSCTSSCTNFGEQPRAGEAGPPAVPGPARRAQAPPEEWERRPQ
  LPPPPRGPPEGPRGAAAPEEEDEEPGDPREGEEEEEEDEPDPEAPENGSLPRFVPRFNFSLKDLTRFVDF
  NIKGRDVIVFLHIQKTGGTTFGRHLVKNIRLEQPCSCKAGQKKCTCHRPGKKETWLFSRFSTGWSCGLHA
  DWTELTNCVPAIMEKKDCPRNHSHTRNFYYITMLRDPVSRYLSEWKHVQRGATWKTSLHMCDGRSPTPDE
  LPTCYPGDDWSGVSLREFMDCTYNLANNRQVRMLADLSLVGCYNLTFMNESERNTILLQSAKNNLKNMAF
  FGLTEFQRKTQFLFERTFNLKFISPFTQFNITRASNVEINEGARQRIEDLNFLDMQLYEYAKDLFQQRYH
  HTKQLEHQRDRQKRREERRLQREHRDHQWPKEDGAAEGTVTEDYNSQVVRW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153456
• ORF Size: 1413 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153456.3
• RefSeq Size: 7817 bp
• RefSeq ORF: 1416 bp
• Locus ID: 266722
• UniProt ID: Q8IZP7
• Cytogenetics: 13q32.1
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis
• Gene Summary: Heparan sulfate (HS) sulfotransferases, such as HS6ST3, modify HS to generate structures required for interactions between HS and a variety of proteins. These interactions are implicated in proliferation and differentiation, adhesion, migration, inflammation, blood coagulation, and other diverse processes (Habuchi et al., 2000 [PubMed 10644753]).[supplied by OMIM, Mar 2008]