HCN1 (NM_021072) Human Tagged ORF Clone

CAT#: RG220956

HCN1 (tGFP-tagged) - Human hyperpolarization activated cyclic nucleotide-gated potassium channel 1 (HCN1)

Product Images

Circular map for RG220956

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HCN1
• Synonyms: BCNG-1; BCNG1; DEE24; EIEE24; GEFSP10; HAC-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220956 representing NM_021072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGAGGCGGCAAGCCCAACTCTTCGTCTAACAGCCGGGACGATGGCAACAGCGTCTTCCCCGCCA
  AGGCGTCCGCGACGGGCGCGGGGCCGGCCGCGGCCGAGAAGCGCCTGGGCACCCCGCCGGGGGGCGGCGG
  GGCCGGCGCGAAGGAGCACGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGTGGCGGCGGTGGCGGCGGC
  GGCGGCGGCGGCGAGGAGCCGGCGGGGGGCTTCGAAGACGCCGAGGGGCCCCGGCGGCAGTACGGCTTCA
  TGCAGAGGCAGTTCACCTCCATGCTGCAGCCCGGGGTCAACAAATTCTCCCTCCGCATGTTTGGGAGCCA
  GAAGGCGGTGGAAAAGGAGCAGGAAAGGGTTAAAACTGCAGGCTTCTGGATTATCCACCCTTACAGTGAT
  TTCAGGTTTTACTGGGATTTAATAATGCTTATAATGATGGTTGGAAATCTAGTCATCATACCAGTTGGAA
  TCACATTCTTTACAGAGCAAACAACAACACCATGGATTATTTTCAATGTGGCATCAGATACAGTTTTCCT
  ATTGGACCTGATCATGAATTTTAGGACTGGGACTGTCAATGAAGACAGTTCTGAAATCATCCTGGACCCC
  AAAGTGATCAAGATGAATTATTTAAAAAGCTGGTTTGTGGTTGACTTCATCTCATCCATCCCAGTGGATT
  ATATCTTTCTTATTGTAGAAAAAGGAATGGATTCTGAAGTTTACAAGACAGCCAGGGCACTTCGCATTGT
  GAGGTTTACAAAAATTCTCAGTCTCTTGCGTTTATTACGACTTTCAAGGTTAATTAGATACATACATCAA
  TGGGAAGAGATATTCCACATGACATATGATCTCGCCAGTGCAGTGGTGAGAATTTTTAATCTCATCGGCA
  TGATGCTGCTCCTGTGCCACTGGGATGGTTGTCTTCAGTTCTTAGTACCACTACTGCAGGACTTCCCACC
  AGATTGCTGGGTGTCTTTAAATGAAATGGTTAATGATTCTTGGGGAAAGCAGTATTCATACGCACTCTTC
  AAAGCTATGAGTCACATGCTGTGCATTGGGTATGGAGCCCAAGCCCCAGTCAGCATGTCTGACCTCTGGA
  TTACCATGCTGAGCATGATCGTCGGGGCCACCTGCTATGCCATGTTTGTCGGCCATGCCACCGCTTTAAT
  CCAGTCTCTGGATTCTTCGAGGCGGCAGTATCAAGAGAAGTATAAGCAAGTGGAACAATACATGTCATTC
  CATAAGTTACCAGCTGATATGCGTCAGAAGATACATGATTACTATGAACACAGATACCAAGGCAAAATCT
  TTGATGAGGAAAATATTCTCAATGAACTCAATGATCCTCTGAGAGAGGAGATAGTCAACTTCAACTGTCG
  GAAACTGGTGGCTACAATGCCTTTATTTGCTAATGCGGATCCTAATTTTGTGACTGCCATGCTGAGCAAG
  TTGAGATTTGAGGTGTTTCAACCTGGAGATTATATCATACGAGAAGGAGCCGTGGGTAAAAAAATGTATT
  TCATTCAACACGGTGTTGCTGGTGTCATTACAAAATCCAGTAAAGAAATGAAGCTGACAGATGGCTCTTA
  CTTTGGAGAGATTTGCCTGCTGACCAAAGGACGTCGTACTGCCAGTGTTCGAGCTGATACATATTGTCGT
  CTTTACTCACTTTCCGTGGACAATTTCAACGAGGTCCTGGAGGAATATCCAATGATGAGGAGAGCCTTTG
  AGACAGTTGCCATTGACCGACTAGATCGAATAGGAAAGAAAAATTCAATTCTTCTGCAAAAGTTCCAGAA
  GGATCTGAACACTGGTGTTTTCAACAATCAGGAGAACGAAATCCTCAAGCAGATTGTGAAACATGACAGG
  GAGATGGTGCAGGCAATCGCTCCCATCAATTATCCTCAAATGACAACCCTGAATTCCACATCGTCTACTA
  CGACCCCGACCTCCCGCATGAGGACACAATCTCCACCGGTGTACACAGCGACCAGCCTGTCTCACAGCAA
  CCTGCACTCCCCCAGTCCCAGCACACAGACCCCCCAGCCATCAGCCATCCTGTCACCCTGCTCCTACACC
  ACCGCGGTCTGCAGCCCTCCTGTACAGAGCCCTCTGGCCGCTCGAACTTTCCACTATGCCTCCCCCACCG
  CCTCCCAGCTGTCACTCATGCAACAGCAGCCGCAGCAGCAGGTACAGCAGTCCCAGCCGCCGCAGACTCA
  GCCACAGCAGCCGTCCCCGCAGCCACAGACACCTGGCAGCTCCACGCCGAAAAATGAAGTGCACAAGAGC
  ACGCAGGCGCTTCACAACACCAACCTGACCCGGGAAGTCAGGCCACTCTCCGCCTCGCAGCCCTCGCTGC
  CCCATGAGGTGTCCACTCTGATTTCCAGACCTCATCCCACTGTGGGCGAGTCCCTGGCCTCCATCCCTCA
  ACCCGTGACGGCGGTCCCCGGAACGGGCCTTCAGGCAGGGGGCAGGAGCACTGTCCCGCAGCGCGTCACC
  CTCTTCCGACAGATGTCGTCGGGAGCCATCCCCCCGAACCGAGGAGTCCCTCCAGCACCCCCTCCACCAG
  CAGCTGCTCTTCCAAGAGAATCTTCCTCAGTCTTAAACACAGACCCAGACGCAGAAAAGCCACGATTTGC
  TTCAAATTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220956 representing NM_021072
  Red=Cloning site Green=Tags(s)
  
  MEGGGKPNSSSNSRDDGNSVFPAKASATGAGPAAAEKRLGTPPGGGGAGAKEHGNSVCFKVDGGGGGGGG
  GGGGEEPAGGFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSD
  FRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDP
  KVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQ
  WEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALF
  KAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSF
  HKLPADMRQKIHDYYEHRYQGKIFDEENILNELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSK
  LRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCR
  LYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDR
  EMVQAIAPINYPQMTTLNSTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYT
  TAVCSPPVQSPLAARTFHYASPTASQLSLMQQQPQQQVQQSQPPQTQPQQPSPQPQTPGSSTPKNEVHKS
  TQALHNTNLTREVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPVTAVPGTGLQAGGRSTVPQRVT
  LFRQMSSGAIPPNRGVPPAPPPPAAALPRESSSVLNTDPDAEKPRFASNL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021072
• ORF Size: 2670 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021072.4
• RefSeq Size: 9685 bp
• RefSeq ORF: 2673 bp
• Locus ID: 348980
• UniProt ID: O60741
• Cytogenetics: 5p12
• Protein Families: Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane
• Gene Summary: The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. [provided by RefSeq, Oct 2011]