DAAM2 (NM_015345) Human Tagged ORF Clone

CAT#: RG220800

DAAM2 (tGFP-tagged) - Human dishevelled associated activator of morphogenesis 2 (DAAM2), transcript variant 2

Product Images

Circular map for RG220800

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DAAM2
• Synonyms: dJ90A20A.1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220800 representing NM_015345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCCGCAAGAGGAGCCACCATGGCCTGGGCTTCCTGTGCTGCTTCGGGGGCAGTGACATCCCCG
  AAATCAACCTCCGGGACAACCACCCTCTGCAGTTCATGGAGTTCTCCAGCCCCATCCCGAACGCAGAGGA
  GCTCAACATCCGCTTTGCAGAGCTGGTGGATGAATTGGATCTCACTGACAAAAACCGAGAGGCTATGTTT
  GCACTGCCCCCTGAGAAGAAATGGCAGATCTACTGCAGCAAGAAGAAGGAGCAGGAGGACCCCAACAAGC
  TGGCAACCAGCTGGCCTGACTATTACATCGACCGCATCAATTCCATGGCTGCGATGCAGAGTCTGTACGC
  GTTTGATGAGGAGGAGACGGAGATGAGGAACCAAGTCGTGGAAGACCTGAAGACAGCCCTCCGGACACAG
  CCTATGAGGTTTGTGACCCGCTTCATTGAGCTGGAGGGCTTGACCTGTCTGCTAAATTTCCTCCGGAGCA
  TGGACCACGCCACCTGTGAGAGCCGCATCCACACCTCACTCATTGGCTGCATCAAAGCATTGATGAACAA
  CTCCCAGGGGCGGGCACATGTGCTGGCACAGCCTGAGGCCATTAGTACCATAGCCCAGAGCCTACGCACA
  GAGAACAGCAAGACCAAGGTGGCTGTGCTGGAGATCCTGGGTGCTGTGTGCCTCGTGCCTGGTGGCCACA
  AGAAGGTGCTGCAGGCCATGCTGCACTACCAGGTGTATGCAGCAGAGCGAACCCGCTTCCAGACCCTGCT
  GAACGAGCTAGACCGAAGTCTGGGCCGGTACCGGGATGAAGTGAATCTGAAAACAGCCATCATGTCCTTC
  ATCAATGCTGTCCTCAATGCTGGAGCTGGAGAGGATAATCTGGAGTTCCGCCTACATCTACGGTATGAAT
  TCCTGATGCTGGGTATACAGCCTGTGATTGACAAGCTCCGGCAACATGAAAATGCCATCCTGGACAAACA
  TTTAGACTTCTTCGAGATGGTGCGGAATGAGGATGACCTGGAGCTAGCCAGGAGGTTTGACATGGTCCAC
  ATCGACACCAAGAGTGCTTCCCAGATGTTTGAGTTGATCCACAAGAAGCTGAAGTACACGGAGGCCTACC
  CCTGCCTGCTCTCTGTGCTGCACCACTGCCTGCAGATGCCCTACAAACGGAACGGTGGCTACTTCCAGCA
  GTGGCAGCTCCTGGACCGCATCCTCCAGCAGATTGTCCTCCAGGATGAGCGGGGTGTGGACCCTGACCTG
  GCTCCCTTGGAGAACTTCAATGTCAAGAACATCGTCAACATGCTCATCAACGAGAATGAAGTGAAACAGT
  GGCGAGACCAGGCAGAGAAGTTCCGGAAAGAACACATGGAGCTTGTGAGCCGTCTGGAGAGGAAGGAGCG
  GGAATGCGAGACAAAGACATTGGAGAAGGAAGAGATGATGCGGACGCTGAACAAAATGAAGGACAAGCTG
  GCCCGGGAGTCCCAGGAGCTGCGCCAGGCTCGGGGACAAGTGGCAGAGCTGGTAGCCCAGCTCAGTGAAC
  TCTCAACAGGCCCTGTATCTTCCCCACCACCCCCTGGGGGCCCACTCACCTTGTCTTCCTCAATGACAAC
  CAATGACCTGCCTCCACCCCCTCCTCCTCTGCCCTTTGCCTGTTGTCCCCCTCCCCCACCACCACCCCTT
  CCTCCCGGGGGACCCCCGACTCCCCCAGGTGCCCCACCTTGCCTCGGCATGGGCCTGCCCCTCCCTCAGG
  ACCCCTACCCCAGCAGTGACGTCCCACTCAGGAAAAAGCGTGTCCCCCAGCCTTCTCACCCACTGAAGTC
  CTTCAACTGGGTGAAGCTGAATGAGGAGCGTGTCCCTGGCACCGTATGGAATGAGATTGATGACATGCAG
  GTATTTCGGATCCTGGACCTAGAGGATTTTGAAAAGATGTTTTCAGCCTACCAGAGGCACCAGAAAGAGC
  TGGGCTCCACTGAAGACATCTACCTGGCTTCCCGCAAGGTCAAAGAGCTGTCGGTCATTGATGGCCGGAG
  GGCCCAAAACTGCATCATCCTTCTTTCCAAGTTGAAGCTTTCTAACGAGGAGATCCGGCAGGCCATCTTG
  AAGATGGATGAGCAGGAGGACCTTGCTAAGGACATGCTGGAGCAGCTCCTCAAGTTCATCCCAGAGAAGA
  GTGACATTGACCTCCTGGAGGAGCACAAGCATGAAATTGAGCGGATGGCCCGTGCTGACCGCTTCCTCTA
  TGAAATGAGCAGGATTGACCACTACCAGCAGCGACTGCAAGCCCTCTTCTTCAAGAAGAAATTCCAGGAG
  CGGCTGGCTGAGGCAAAGCCCAAAGTGGAAGCCATCCTGTTGGCCTCCCGGGAGCTGGTCCGCAGCAAGC
  GTCTTAGACAGATGCTAGAGGTCATCCTAGCCATAGGCAACTTCATGAACAAAGGGCAGCGTGGGGGCGC
  CTACGGGTTCCGGGTGGCCAGCCTCAACAAGATCGCTGACACCAAGTCCAGCATCGACAGAAACATCTCT
  CTGCTCCATTACCTGATCATGATCCTGGAGAAGCATTTTCCTGATATTCTAAACATGCCTTCAGAGCTGC
  AACATCTTCCAGAAGCTGCCAAAGTCAACCTAGCAGAACTGGAGAAGGAGGTGGGCAACCTCAGGAGGGG
  CCTGAGAGCGGTGGAGGTGCTGGAGTATCAGAGGCGCCAGGTACGGGAGCCCAGTGACAAGTTTGTCCCT
  GTCATGAGCGACTTCATCACGGTGTCCAGCTTCAGCTTCTCCGAGCTGGAGGACCAGCTAAATGAGGCCA
  GGGACAAGTTCGCCAAGGCCTTGATGCACTTCGGGGAGCATGACAGCAAGATGCAGCCAGACGAATTCTT
  TGGCATCTTTGATACCTTCTTGCAGGCCTTCTCAGAGGCCCGGCAGGATCTAGAGGCCATGAGGAGGAGG
  AAGGAGGAGGAGGAGCGGCGGGCGCGCATGGAAGCCATGCTGAAGGAGCAGAGGGAACGTGAGCGGTGGC
  AGCGGCAGCGGAAGGTCCTGGCTGCAGGCAGCTCGCTGGAGGAGGGAGGAGAGTTCGATGACCTGGTGTC
  GGCCCTGCGCTCTGGGGAGGTCTTCGACAAGGACTTATGCAAGCTCAAGCGCAGCCGCAAGCGATCAGGG
  AGCCAGGCCCTGGAAGTTACCCGGGAGCGGGCAATAAACCGGCTAAATTAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220800 representing NM_015345
  Red=Cloning site Green=Tags(s)
  
  MAPRKRSHHGLGFLCCFGGSDIPEINLRDNHPLQFMEFSSPIPNAEELNIRFAELVDELDLTDKNREAMF
  ALPPEKKWQIYCSKKKEQEDPNKLATSWPDYYIDRINSMAAMQSLYAFDEEETEMRNQVVEDLKTALRTQ
  PMRFVTRFIELEGLTCLLNFLRSMDHATCESRIHTSLIGCIKALMNNSQGRAHVLAQPEAISTIAQSLRT
  ENSKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQVYAAERTRFQTLLNELDRSLGRYRDEVNLKTAIMSF
  INAVLNAGAGEDNLEFRLHLRYEFLMLGIQPVIDKLRQHENAILDKHLDFFEMVRNEDDLELARRFDMVH
  IDTKSASQMFELIHKKLKYTEAYPCLLSVLHHCLQMPYKRNGGYFQQWQLLDRILQQIVLQDERGVDPDL
  APLENFNVKNIVNMLINENEVKQWRDQAEKFRKEHMELVSRLERKERECETKTLEKEEMMRTLNKMKDKL
  ARESQELRQARGQVAELVAQLSELSTGPVSSPPPPGGPLTLSSSMTTNDLPPPPPPLPFACCPPPPPPPL
  PPGGPPTPPGAPPCLGMGLPLPQDPYPSSDVPLRKKRVPQPSHPLKSFNWVKLNEERVPGTVWNEIDDMQ
  VFRILDLEDFEKMFSAYQRHQKELGSTEDIYLASRKVKELSVIDGRRAQNCIILLSKLKLSNEEIRQAIL
  KMDEQEDLAKDMLEQLLKFIPEKSDIDLLEEHKHEIERMARADRFLYEMSRIDHYQQRLQALFFKKKFQE
  RLAEAKPKVEAILLASRELVRSKRLRQMLEVILAIGNFMNKGQRGGAYGFRVASLNKIADTKSSIDRNIS
  LLHYLIMILEKHFPDILNMPSELQHLPEAAKVNLAELEKEVGNLRRGLRAVEVLEYQRRQVREPSDKFVP
  VMSDFITVSSFSFSELEDQLNEARDKFAKALMHFGEHDSKMQPDEFFGIFDTFLQAFSEARQDLEAMRRR
  KEEEERRARMEAMLKEQRERERWQRQRKVLAAGSSLEEGGEFDDLVSALRSGEVFDKDLCKLKRSRKRSG
  SQALEVTRERAINRLNY
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015345
• ORF Size: 3201 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015345.3
• RefSeq Size: 6215 bp
• RefSeq ORF: 3204 bp
• Locus ID: 23500
• UniProt ID: Q86T65
• Cytogenetics: 6p21.2
• Protein Pathways: Wnt signaling pathway
• Gene Summary: Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system. Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity. During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A. Also regulates non-canonical Wnt signaling pathway. Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery: affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation. Together with DAAM1, required for myocardial maturation and sarcomere assembly.[UniProtKB/Swiss-Prot Function]