PODXL (NM_001018111) Human Tagged ORF Clone

CAT#: RG219976

PODXL (tGFP-tagged) - Human podocalyxin-like (PODXL), transcript variant 1

Product Images

Circular map for RG219976

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PODXL
• Synonyms: gp135; Gp200; PC; PCLP; PCLP-1; PDX; PODXL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219976 representing NM_001018111
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCTGCGCGCTGGCGCTCTCGGCGCTGCTGCTACTGTTGTCAACGCCGCCGCTGCTGCCGTCGTCGC
  CGTCGCCGTCGCCGTCGCCCTCCCAGAATGCAACCCAGACTACTACGGACTCATCTAACAAAACAGCACC
  GACTCCAGCATCCAGTGTCACCATCATGGCTACAGATACAGCCCAGCAGAGCACAGTCCCCACTTCCAAG
  GCCAACGAAATCTTGGCCTCGGTCAAGGCGACCACCCTTGGTGTATCCAGTGACTCACCGGGGACTACAA
  CCCTGGCTCAGCAAGTCTCAGGCCCAGTCAACACTACCGTGGCTAGAGGAGGCGGCTCAGGCAACCCTAC
  TACCACCATCGAGAGCCCCAAGAGCACAAAAAGTGCAGACACCACTACAGTTGCAACCTCCACAGCCACA
  GCTAAACCTAACACCACAAGCAGCCAGAATGGAGCAGAAGATACAACAAACTCTGGGGGGAAAAGCAGCC
  ACAGTGTGACCACAGACCTCACATCCACTAAGGCAGAACATCTGACGACCCCTCACCCTACAAGTCCACT
  TAGCCCCCGACAACCCACTTCGACGCATCCTGTGGCCACCCCAACAAGCTCGGGACATGACCATCTTATG
  AAAATTTCAAGCAGTTCAAGCACTGTGGCTATCCCTGGCTACACCTTCACAAGCCCGGGGATGACCACCA
  CCCTACTAGAGACAGTGTTTCACCATGTCAGCCAGGCTGGTCTTGAACTCCTGACCTCGGGTGATCTGCC
  CACCTTGGCCTCCCAAAGTGCTGGGATTACAGCGTCATCGGTTATCTCGCAAAGAACTCAACAGACCTCC
  AGTCAGATGCCAGCCAGCTCTACGGCCCCTTCCTCCCAGGAGACAGTGCAGCCCACGAGCCCGGCAACGG
  CATTGAGAACACCTACCCTGCCAGAGACCATGAGCTCCAGCCCCACAGCAGCATCAACTACCCACCGATA
  CCCCAAAACACCTTCTCCCACTGTGGCTCATGAGAGTAACTGGGCAAAGTGTGAGGATCTTGAGACACAG
  ACACAGAGTGAGAAGCAGCTCGTCCTGAACCTCACAGGAAACACCCTCTGTGCAGGGGGCGCTTCGGATG
  AGAAATTGATCTCACTGATATGCCGAGCAGTCAAAGCCACCTTCAACCCGGCCCAAGATAAGTGCGGCAT
  ACGGCTGGCATCTGTTCCAGGAAGTCAGACCGTGGTCGTCAAAGAAATCACTATTCACACTAAGCTCCCT
  GCCAAGGATGTGTACGAGCGGCTGAAGGACAAATGGGATGAACTAAAGGAGGCAGGGGTCAGTGACATGA
  AGCTAGGGGACCAGGGGCCACCGGAGGAGGCCGAGGACCGCTTCAGCATGCCCCTCATCATCACCATCGT
  CTGCATGGCATCATTCCTGCTCCTCGTGGCGGCCCTCTATGGCTGCTGCCACCAGCGCCTCTCCCAGAGG
  AAGGACCAGCAGCGGCTAACAGAGGAGCTGCAGACAGTGGAGAATGGTTACCATGACAACCCAACACTGG
  AAGTGATGGAGACCTCTTCTGAGATGCAGGAGAAGAAGGTGGTCAGCCTCAACGGGGAGCTGGGGGACAG
  CTGGATCGTCCCTCTGGACAACCTGACCAAGGACGACCTGGATGAGGAGGAAGACACACACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219976 representing NM_001018111
  Red=Cloning site Green=Tags(s)
  
  MRCALALSALLLLLSTPPLLPSSPSPSPSPSQNATQTTTDSSNKTAPTPASSVTIMATDTAQQSTVPTSK
  ANEILASVKATTLGVSSDSPGTTTLAQQVSGPVNTTVARGGGSGNPTTTIESPKSTKSADTTTVATSTAT
  AKPNTTSSQNGAEDTTNSGGKSSHSVTTDLTSTKAEHLTTPHPTSPLSPRQPTSTHPVATPTSSGHDHLM
  KISSSSSTVAIPGYTFTSPGMTTTLLETVFHHVSQAGLELLTSGDLPTLASQSAGITASSVISQRTQQTS
  SQMPASSTAPSSQETVQPTSPATALRTPTLPETMSSSPTAASTTHRYPKTPSPTVAHESNWAKCEDLETQ
  TQSEKQLVLNLTGNTLCAGGASDEKLISLICRAVKATFNPAQDKCGIRLASVPGSQTVVVKEITIHTKLP
  AKDVYERLKDKWDELKEAGVSDMKLGDQGPPEEAEDRFSMPLIITIVCMASFLLLVAALYGCCHQRLSQR
  KDQQRLTEELQTVENGYHDNPTLEVMETSSEMQEKKVVSLNGELGDSWIVPLDNLTKDDLDEEEDTHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001018111
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001018111.3
• RefSeq Size: 5999 bp
• RefSeq ORF: 1677 bp
• Locus ID: 5420
• UniProt ID: O00592
• Cytogenetics: 7q32.3
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]