Synapsin III (SYN3) (NM_003490) Human Tagged ORF Clone

CAT#: RG219395

SYN3 (tGFP-tagged) - Human synapsin III (SYN3), transcript variant IIIa

Product Images

Circular map for RG219395

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Synapsin III
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219395 representing NM_003490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTCCTCCGGCGACGTCTCTCTGACAGCAGCTTCATGGCCAACCTGCCTAATGGCTATATGACGG
  ACCTGCAACGCCCAGATAGCTCCACCAGCTCACCTGCTTCCCCCGCCATGGAGAGGAGGCACCCCCAGCC
  CCTGGCTGCCTCCTTCTCCTCTCCAGGATCCAGCCTTTTTAGCTCCCTCTCCAGTGCCATGAAGCAGGCC
  CCTCAGGCCACCTCAGGACTGATGGAGCCTCCAGGTCCCTCCACGCCCATTGTTCAAAGACCCAGGATCC
  TGTTGGTGATCGATGATGCCCATACAGACTGGTCGAAGTATTTCCATGGGAAGAAGGTGAATGGAGAGAT
  TGAGATCCGAGTGGAGCAGGCTGAATTCTCAGAGTTGAACCTAGCTGCCTATGTGACCGGGGGCTGCATG
  GTGGACATGCAGGTCGTGAGAAATGGGACCAAAGTGGTGAGATCCTTCAAGCCAGACTTCATCCTGGTCC
  GCCAGCATGCCTACAGCATGGCCCTGGGGGAAGACTACCGCAGCCTGGTCATCGGCCTGCAGTATGGAGG
  GCTGCCTGCTGTCAACTCTCTCTACTCCGTCTACAACTTCTGCAGCAAGCCCTGGGTGTTCTCTCAGCTC
  ATTAAGATCTTCCATTCCCTGGGTCCTGAGAAGTTCCCGCTTGTGGAGCAAACATTTTTCCCCAACCATA
  AGCCAATGGTCACAGCCCCACACTTCCCGGTGGTAGTCAAGCTGGGACATGCCCACGCTGGAATGGGAAA
  GATCAAAGTGGAAAACCAGCTTGACTTCCAGGACATCACCAGCGTGGTCGCCATGGCCAAAACCTACGCC
  ACCACCGAGGCCTTCATCGACTCCAAGTACGACATCCGCATCCAGAAAATTGGATCCAACTACAAGGCTT
  ACATGAGAACCTCCATCTCTGGGAACTGGAAGGCCAACACAGGCTCTGCCATGCTGGAGCAGGTGGCCAT
  GACAGAGAGGTACAGGCTGTGGGTGGACAGCTGCTCGGAAATGTTTGGCGGCCTGGACATCTGTGCCGTC
  AAGGCTGTCCACAGCAAGGATGGCAGAGATTACATCATCGAGGTAATGGACAGCTCAATGCCGCTGATTG
  GAGAGCATGTGGAAGAGGACAGACAGCTGATGGCCGACCTTGTTGTCTCCAAAATGAGCCAGCTCCCGAT
  GCCAGGAGGCACAGCGCCCTCCCCCCTCAGACCTTGGGCTCCACAGATTAAATCAGCGAAATCCCCAGGG
  CAAGCCCAGCTGGGGCCTCAGCTAGGCCAGCCCCAGCCACGCCCACCTCCGCAAGGAGGCCCTCGCCAAG
  CTCAGTCTCCTCAGCCCCAGAGATCTGGAAGCCCCTCCCAACAGAGGCTCTCCCCACAAGGCCAGCAGCC
  CCTGAGCCCCCAGTCCGGATCTCCACAGCAGCAAAGGTCACCAGGCTCTCCGCAGCTATCCCGGGCATCC
  AGTGGCAGCTCCCCAAACCAGGCCTCCAAGCCAGGTGCCACCCTCGCCTCACAGCCCCGGCCCCCTGTGC
  AGGGCCGTAGTACCTCCCAGCAGGGTGAAGAGTCCAAGAAGCCAGCACCACCCCATCCGCATCTCAACAA
  ATCTCAGTCCCTGACTAACAGCCTCAGCACATCCGACACCTCCCAGCGTGGGACCCCAAGTGAAGACGAG
  GCCAAGGCTGAAACCATCCGCAACCTGAGGAAGTCTTTTGCCAGCCTGTTCTCTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219395 representing NM_003490
  Red=Cloning site Green=Tags(s)
  
  MNFLRRRLSDSSFMANLPNGYMTDLQRPDSSTSSPASPAMERRHPQPLAASFSSPGSSLFSSLSSAMKQA
  PQATSGLMEPPGPSTPIVQRPRILLVIDDAHTDWSKYFHGKKVNGEIEIRVEQAEFSELNLAAYVTGGCM
  VDMQVVRNGTKVVRSFKPDFILVRQHAYSMALGEDYRSLVIGLQYGGLPAVNSLYSVYNFCSKPWVFSQL
  IKIFHSLGPEKFPLVEQTFFPNHKPMVTAPHFPVVVKLGHAHAGMGKIKVENQLDFQDITSVVAMAKTYA
  TTEAFIDSKYDIRIQKIGSNYKAYMRTSISGNWKANTGSAMLEQVAMTERYRLWVDSCSEMFGGLDICAV
  KAVHSKDGRDYIIEVMDSSMPLIGEHVEEDRQLMADLVVSKMSQLPMPGGTAPSPLRPWAPQIKSAKSPG
  QAQLGPQLGQPQPRPPPQGGPRQAQSPQPQRSGSPSQQRLSPQGQQPLSPQSGSPQQQRSPGSPQLSRAS
  SGSSPNQASKPGATLASQPRPPVQGRSTSQQGEESKKPAPPHPHLNKSQSLTNSLSTSDTSQRGTPSEDE
  AKAETIRNLRKSFASLFSD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003490
• ORF Size: 1737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003490.2, NP_003481.2
• RefSeq Size: 2918 bp
• RefSeq ORF: 1743 bp
• Locus ID: 8224
• UniProt ID: O14994
• Cytogenetics: 22q12.3
• Protein Families: Secreted Protein
• Gene Summary: This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. The protein encoded by this gene shares the synapsin family domain model, with domains A, C, and E exhibiting the highest degree of conservation. The protein contains a unique domain J, located between domains C and E. Based on this gene's localization to 22q12.3, a possible schizophrenia susceptibility locus, and the established neurobiological roles of the synapsins, this family member may represent a candidate gene for schizophrenia. The TIMP3 gene is located within an intron of this gene and is transcribed in the opposite direction. Alternative splicing of this gene results in multiple splice variants that encode different isoforms. [provided by RefSeq, Oct 2008]