RNF39 (NM_025236) Human Tagged ORF Clone

CAT#: RG219332

RNF39 (tGFP-tagged) - Human ring finger protein 39 (RNF39), transcript variant 1

Product Images

Circular map for RG219332

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RNF39
• Synonyms: FAP216; HZF; HZFW; LIRF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219332 representing NM_025236
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTGGAGAGATTTGACGAGACTGAGACTCTGGTTGAAGAGAGAGGCAATCCCAGGAGAGGGGCGGA
  AAGCGGCAAAAGTTAATGCGGGAGTCGGAGAGAAGGGCATCTACACAGCAAGCAGCAGGGGCGGCCCGCC
  ATCTGCGCGCTCGAAGGCGGTCACGGTGGTCGCGGAAGGGGCGGCGTCCAGATCCTGGCTTTCCATGGAT
  GCGCCCGAGCTGGGCCCGGGGCTGGTGGAGCGTCTGGAGCAGCTGGCGACGTGTCCTCTGTGCGGGGGCT
  CCTTCGAGGACCCGGTGCTTCTGGCGTGCGAGCACAGCTTCTGCCGCGCGTGTCTGGCCCGCCGCTGGGG
  GACTCCGCCGGCGACCGGCACCGAGGCTTCCCCCACCGCCTGTCCCTGCTGCGGCCTGCCGTGTCCCCGC
  CGCAGCCTGAGGTCTAATGTGCGGCTGGCGGTGGAGGTGCGAATCAGCCGCGAGCTGCGAGAGAAGCTGG
  CTGAGCCTGGGGCCCGTGCGGGGAGACGCCGAGGGGGGCGCATCCCCACCATGGGCTGCCTGGACCTGCC
  CGGAGAGGATATGAGGAAGACATGGAGACGATTTGAAGTCCCAACATCCAAGTCATCTAATTCAGAGGAT
  GATCTCCCTGAAGATTATCCAGTGGTCAAAAAAATGCTTCATAGACTGACAGCCGACCTGACCCTGGACC
  CTGGGACCGCACACCGCCGCCTGCTCATCTCCGCCGACCGCCGCAGCGTACAACTGGCCCCACCAGGGAC
  GCCCGCGCCCCCTGACGGCCCCAAGCGCTTCGATCAGCTCCCAGCTGTGCTGGGTGCGCAGGGCTTCGGG
  GCCGGCCGCCACTGCTGGGAGGTGGAGACTGCGGACGCCGCCTCCTGCAGAGACTCTTCTGGGGAGGATG
  CGGACGACGAGGAGAGCCACTATGCAGTGGGCGCGGCCGGGGAATCAGTGCAACGCAAGGGCTGCGTAAG
  GCTGTGCCCTGCGGGGGCCGTGTGGGCCGTGGAGGGCCGCGGCGGCCGCCTGTGGGCCCTCACGGCACCC
  GAACCCACCCTGCTGGGCGGTGTTGAGCCCCCGCCGCGGCGCATTCGCGTGGACCTGGACTGGGAGCGGG
  GCCGCGTGGCCTTCTACGACGGCCGCTCACTCGACCTGCTTTACGCCTTCCAGGCGCCTGGCCCCCTGGG
  GGAGCGCATCTTCCCGCTGTTCTGCACCTGCGACCCTCGTGCTCCGCTCCGCATTGTACCAGCGGAAAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219332 representing NM_025236
  Red=Cloning site Green=Tags(s)
  
  MWWRDLTRLRLWLKREAIPGEGRKAAKVNAGVGEKGIYTASSRGGPPSARSKAVTVVAEGAASRSWLSMD
  APELGPGLVERLEQLATCPLCGGSFEDPVLLACEHSFCRACLARRWGTPPATGTEASPTACPCCGLPCPR
  RSLRSNVRLAVEVRISRELREKLAEPGARAGRRRGGRIPTMGCLDLPGEDMRKTWRRFEVPTSKSSNSED
  DLPEDYPVVKKMLHRLTADLTLDPGTAHRRLLISADRRSVQLAPPGTPAPPDGPKRFDQLPAVLGAQGFG
  AGRHCWEVETADAASCRDSSGEDADDEESHYAVGAAGESVQRKGCVRLCPAGAVWAVEGRGGRLWALTAP
  EPTLLGGVEPPPRRIRVDLDWERGRVAFYDGRSLDLLYAFQAPGPLGERIFPLFCTCDPRAPLRIVPAES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025236
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025236.3
• RefSeq Size: 2187 bp
• RefSeq ORF: 1059 bp
• Locus ID: 80352
• UniProt ID: Q9H2S5
• Cytogenetics: 6p22.1
• Protein Families: Druggable Genome
• Gene Summary: This gene lies within the major histocompatibility complex class I region on chromosome 6. Studies of a similar rat protein suggest that this gene encodes a protein that plays a role in an early phase of synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]