PI4KA (NM_002650) Human Tagged ORF Clone

CAT#: RG218825

PI4KA (tGFP-tagged) - Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1

Product Images

Circular map for RG218825

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PI4KA
• Synonyms: PI4K-ALPHA; pi4K230; PIK4CA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218825 representing NM_002650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTGGAGCAGAAATTTGGCCTGTTTTCTGCTGAGATAA
  AGGAAGCAGACCCCCTGGCTGCCTCGGAAGCAAGTCAACCCAAACCCTGTCCCCCCGAAGTGACCCCCCA
  CTACATCTGGATCGACTTCCTGGTGCAGCGGTTTGAGATCGCCAAGTACTGCAGCTCTGACCAAGTGGAG
  ATCTTCTCCAGCCTGCTGCAGCGCTCCATGTCCCTGAACATCGGCGGGGCCAAGGGGAGCATGAACCGGC
  ACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGACCCTGGGGCTGTCCCTCCTGCATGCCGATGTGGT
  TCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAAGATCTACTCCACTGCCTTTGACTACTTCAGCTGT
  CCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTGCGTGAAGACATAAGCATCATGATTAAATTTTGGA
  CCGCCATGTTCTCAGATAAGAAGTACCTGACCGCCAGCCAGCTTGTTCCCCCAGATAATCAGGACACCCG
  GAGCAACCTGGACATAACTGTCGGCTCTCGGCAACAAGCCACCCAAGGCTGGATCAACACATACCCCCTG
  TCCAGCGGCATGTCCACCATCTCCAAGAAATCAGGCATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGC
  ACAAATACTACATGAAGCGCAGGACGCTGCTGCTGTCCCTGCTGGCCACTGAGATCGAGCGTCTCATCAC
  ATGGTACAACCCGCTGTCAGCCCCGGAACTGGAACTAGACCAGGCCGGAGAGAACAGCGTGGCCAACTGG
  AGATCTAAGTACATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTCGCCTGGAGCATCTCTC
  CCTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAAGAACACAGAAGCCATTGGGAACGAAGTGACCCGTCT
  CGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCCTGAAGCAATCAAGTTCCTGGTCACCTGGCACACC
  ATCGACGCCGATGCTCCAGAGCTCAGCCATGTGCTGTGCTGGGCGCCCACGGACCCACCCACAGGCCTCT
  CCTACTTCTCCAGCATGTACCCGCCGCACCCTCTCACGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTT
  CCCTCCGGACGCCATCCTCTTCTACATCCCCCAGATTGTGCAGGCCCTCAGGTACGACAAGATGGGCTAT
  GTGCGGGAGTATATTCTGTGGGCAGCGTCTAAATCCCAGCTTCTGGCACACCAGTTCATCTGGAACATGA
  AGACTAACATTTATCTAGATGAAGAGGGCCACCAGAAAGACCCTGACATCGGCGACCTCCTGGATCAGTT
  GGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGCGAAGGACTTTTACCAGCGGGAGTTTGATTTCTTT
  AACAAGATCACCAACGTGTCGGCTATCATCAAGCCCTACCCTAAAGGCGACGAGAGAAAGAAGGCTTGTC
  TGTCGGCCCTGTCTGAAGTGAAGGTGCAGCCGGGCTGCTACCTGCCCAGCAACCCTGAGGCCATTGTGCT
  GGACATCGACTACAAGTCTGGGACCCCGATGCAGAGTGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAG
  GTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAAGGTCTGCGGTGCCGCTCAGACTCCGAGGATGAGT
  GCAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCTGGCAGGCAGCCATCTTCAAGGTGGGAGACGACTG
  CCGGCAGGACATGCTGGCCCTGCAGATCATCGACCTCTTCAAGAACATCTTCCAGCTGGTCGGCCTGGAC
  CTCTTTGTTTTTCCCTACCGCGTGGTGGCCACTGCCCCTGGGTGCGGGGTGATCGAGTGCATCCCCGACT
  GCACCTCCCGGGACCAGCTGGGCCGCCAGACAGACTTCGGCATGTACGACTACTTCACACGCCAGTACGG
  GGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTACAACTTCATCCGAAGCATGGCCGCCTACAGCCTC
  CTGCTGTTCCTGCTGCAGATCAAGGACAGACACAACGGCAACATTATGCTGGACAAGAAGGGTCATATCA
  TCCACATCGACTTTGGCTTCATGTTTGAAAGCTCGCCGGGCGGCAATCTCGGCTGGGAACCCGACATCAA
  GCTGACGGATGAGATGGTGATGATCATGGGGGGCAAGATGGAGGCCACACCCTTCAAGTGGTTCATGGAG
  ATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTACATGGACGCGGTCGTCTCCCTGGTCACTCTCATGT
  TGGACACGGGCCTGCCCTGTTTTCGCGGCCAGACAATCAAGCTCTTGAAGCACAGGTTTAGCCCCAACAT
  GACTGAGCGCGAGGCTGCAAATTTCATCATGAAGGTCATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGG
  ACCTACGACATGATCCAGTACTATCAGAATGACATCCCCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218825 representing NM_002650
  Red=Cloning site Green=Tags(s)
  
  MREMAGAWHMTVEQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVE
  IFSSLLQRSMSLNIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSC
  PPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPL
  SSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANW
  RSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHT
  IDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGY
  VREYILWAASKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFF
  NKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFK
  VKRCGVSELEKEGLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLD
  LFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSL
  LLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFME
  MCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSR
  TYDMIQYYQNDIPY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002650
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002650.2
• RefSeq Size: 3034 bp
• RefSeq ORF: 2565 bp
• Locus ID: 5297
• Cytogenetics: 22q11.21
• Domains: PI3_PI4_kinase, PI3Ka
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2018]