DOT1L (NM_032482) Human Tagged ORF Clone

CAT#: RG218547

DOT1L (tGFP-tagged) - Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)

Product Images

Circular map for RG218547

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DOT1L
• Synonyms: DOT1; KMT4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218547 representing NM_032482
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGAAGCTGGAGCTGAGACTGAAGTCGCCCGTGGGGGCTGAGCCCGCCGTCTACCCGTGGCCGC
  TGCCGGTCTACGATAAACATCACGATGCTGCTCATGAAATCATCGAGACCATCCGATGGGTCTGTGAAGA
  AATCCCGGATCTCAAGCTCGCTATGGAGAATTACGTTTTAATTGACTATGACACCAAAAGCTTCGAGAGC
  ATGCAGAGGCTCTGCGACAAGTACAACCGTGCCATCGACAGCATCCACCAGCTGTGGAAGGGCACCACGC
  AGCCCATGAAGCTGAACACGCGGCCGTCCACTGGACTCCTGCGCCATATCCTGCAGCAGGTCTACAACCA
  CTCGGTGACCGACCCCGAGAAGCTCAACAACTACGAGCCCTTCTCCCCCGAGGTGTACGGGGAGACCTCC
  TTCGACCTGGTGGCCCAGATGATTGATGAGATCAAGATGACCGACGACGACCTGTTTGTGGACTTGGGGA
  GCGGTGTGGGCCAGGTCGTGCTCCAGGTTGCTGCTGCCACCAACTGCAAACATCACTATGGCGTCGAGAA
  AGCAGACATCCCGGCCAAGTATGCGGAGACCATGGACCGCGAGTTCAGGAAGTGGATGAAATGGTATGAA
  AAAAAGCATGCAGAATACACATTGGAGAGAGGCGATTTCCTCTCAGAAGAGTGGAGGGAGCGAATCGCCA
  ACACGAGTGTTATATTTGTGAATAATTTTGCCTTTGGTCCTGAGGTGGATCACCAGCTGAAGGAGCGGTT
  TGCAAACATGAAGGAAGGTGGCAGAATCGTGTCCTCGAAACCCTTTGCACCTCTGAACTTCAGAATAAAC
  AGTAGAAACTTGAGTGACATCGGCACCATCATGCGCGTGGTGGAGCTCTCGCCCCTGAAGGGCTCGGTGT
  CGTGGACGGGGAAGCCAGTCTCCTACTACCTGCACACTATCGACCGCACCATACTTGAAAACTATTTTTC
  TAGTCTGAAAAACCCAAAACTCAGGGAGGAACAGGAGGCAGCCCGGCGCCGCCAGCAGCGCGAGAGCAAG
  AGCAACGCGGCCACGCCCACTAAGGGCCCAGAGGGCAAGGTGGCCGGCCCCGCCGACGCCCCCATGGACT
  CTGGTGCTGAGGAAGAGAAGGCGGGAGCAGCCACCGTGAAGAAGCCGTCTCCCTCCAAAGCCCGCAAGAA
  GAAGCTAAACAAGAAGGGGAGGAAGATGGCTGGCCGCAAGCGCGGGCGCCCCAAGAAGATGAACACTGCG
  AACCCCGAGCGGAAGCCCAAGAAGAACCAAACTGCACTGGATGCCCTGCACGCTCAGACCGTGTCTCAGA
  CGGCGGCCTCCTCACCCCAGGATGCCTACAGATCCCCTCACAGCCCGTTCTACCAGCTACTTCCGAGCGT
  GCAGCGGCACTCCCCCAACCCGCTGCTGGTGGCGCCCACCCCGCCCGCGCTGCAGAAGCTTCTAGAGTCC
  TTCAAGATCCAGTACCTGCAGTTCCTGGCATACACAAAGACCCCCCAGTACAAGGCCAGCCTGCAGGAGC
  TGCTGGGCCAGGAGAAGGAGAAGAACGCCCAGCTCCTGGGTGCGGCTCAGCAGCTCCTCAGCCACTGCCA
  GGCCCAGAAGGAGGAGATCAGGAGGCTGTTTCAGCAAAAATTGGATGAGCTGGGTGTGAAGGCGCTGACC
  TACAACGACCTGATTCAAGCGCAGAAGGAGATCTCCGCCCATAACCAGCAGCTGCGGGAGCAGTCGGAGC
  AGCTGGAGCAGGACAACCGCGCGCTCCGCGGCCAGAGCTTGCAGCTGCTCAAGGCTCGCTGCGAGGAGCT
  GCAGCTGGACTGGGCCACGCTGTCGCTGGAGAAGCTGTTGAAGGAGAAGCAGGCCCTGAAGAGCCAGATC
  TCGGAGAAGCAGAGGCACTGCCTGGAGCTGCAGATCAGCATTGTGGAGCTAGAGAAGAGCCAGCGGCAGC
  AGGAGCTCCTGCAGCTCAAGTCCTGTGTGCCGCCTGACGACGCCCTGTCCCTGCACCTGCGTGGGAAGGG
  CGCCCTGGGCCGCGAGCTGGAGCCTGACGCCAGCCGGCTGCACCTGGAGCTGGACTGCACCAAGTTCTCG
  CTGCCTCACTTGAGCAGCATGAGCCCGGAGCTCTCCATGAACGGCCAGGCTGCTGGCTATGAGCTCTGCG
  GTGTGCTGAGCCGGCCTTCGTCGAAGCAGAACACGCCCCAGTACCTGGCCTCACCCCTGGACCAGGAGGT
  GGTGCCCTGTACCCCTAGCCACGTCGGCCGGCCGCGCCTGGAGAAGCTGTCTGGCCTAGCCGCACCCGAC
  TACACTAGGCTGTCCCCGGCCAAGATTGTGCTGAGGCGGCACCTGAGCCAGGACCACACGGTGCCCGGCA
  GGCCGGCTGCCAGTGAGCTGCATTCGAGAGCTGAGCACACCAAGGAGAACGGCCTTCCCTACCAGAGCCC
  CAGCGTGCCTGGCAGCATGAAGCTGAGCCCTCAGGACCCGCGGCCCCTGTCCCCTGGGGCCTTGCAGCTT
  GCTGGAGAGAAGAGCAGTGAGAAGGGCCTGAGAGAGCGCGCCTACGGCAGCAGCGGGGAGCTCATCACCA
  GCCTGCCCATCAGCATCCCGCTCAGCACCGTGCAGCCCAACAAGCTCCCGGTCAGCATTCCCCTGGCCAG
  CGTGGTGCTGCCCAGCCGCGCCGAGAGGGCGAGGAGCACCCCCAGTCCCGTGCTGCAGCCCCGTGACCCC
  TCGTCCACACTTGAAAAGCAGATTGGTGCTAATGCCCACGGTGCTGGGAGCAGAAGCCTTGCCCTGGCCC
  CCGCAGGCTTCTCCTACGCTGGCTCGGTGGCCATCAGCGGGGCCTTGGCGGGCAGCCCGGCCTCTCTCAC
  ACCTGGAGCCGAGCCGGCCACCTTGGATGAGTCCTCCAGCTCTGGGAGCCTTTTTGCCACCGTGGGGTCC
  CGCAGCTCCACGCCACAGCACCCCCTGCTGCTGGCACAGCCCCGGAACTCGCTTCCTGCCTCTCCCGCCC
  ACCAGCTCTCCTCCAGTCCCCGGCTTGGTGGGGCCGCCCAGGGCCCGTTGCCCGAGGCCAGCAAGGGAGA
  CCTGCCCTCCGATTCCGGCTTCTCAGATCCTGAGAGTGAAGCCAAGAGGAGGATTGTGTTCACCATCACC
  ACTGGTGCGGGCAGTGCCAAGCAGTCGCCCTCCAGCAAGCACAGCCCCCTGACCGCCAGCGCCCGTGGGG
  ACTGTGTGCCGAGCCACGGGCAGGACAGTCGCAGGCGCGGCCGGCGGAAGCGAGCATCTGCGGGGACGCC
  CAGCTTGAGCGCAGGCGTGTCCCCCAAGCGCCGAGCCCTGCCGTCCGTCGCTGGCCTTTTCACACAGCCT
  TCGGGGTCTCCCCTCAACCTCAACTCCATGGTCAGTAACATCAACCAGCCCCTGGAGATTACAGCCATCT
  CGTCCCCGGAGACCTCCCTGAAGAGCTCCCCTGTGCCCTACCAGGACCACGACCAGCCCCCCGTGCTCAA
  GAAGGAGCGGCCTCTGAGCCAGACCAATGGGGCACACTACTCCCCACTCACCTCAGACGAGGAGCCAGGC
  TCTGAGGACGAGCCCAGCAGTGCTCGAATTGAGAGAAAAATTGCAACAATCTCCTTAGAAAGCAAATCTC
  CCCCGAAAACCTTGGAAAATGGTGGTGGCTTGGCGGGAAGGAAGCCCGCGCCCGCCGGCGAGCCAGTCAA
  TAGCAGCAAGTGGAAGTCCACCTTCTCGCCCATCTCCGACATCGGCCTGGCCAAGTCGGCGGACAGCCCG
  CTGCAGGCCAGCTCCGCCCTCAGCCAGAACTCCCTGTTCACGTTCCGGCCCGCCCTGGAGGAGCCCTCTG
  CCGATGCCAAGCTGGCCGCTCACCCCAGGAAAGGCTTTCCCGGCTCCCTGTCGGGGGCTGACGGACTCAG
  CCCGGGCACCAACCCTGCCAACGGCTGCACCTTCGGCGGGGGCCTGGCCGCGGACCTGAGTTTACACAGC
  TTCAGTGATGGTGCTTCTCTTCCCCACAAGGGCCCCGAGGCGGCCGGCCTGAGCTCCCCGCTGAGCTTCC
  CCTCGCAGCGCGGCAAGGAGGGCTCGGACGCCAACCCTTTCCTGAGCAAGAGGCAGCTGGACGGCCTGGC
  TGGGCTGAAGGGCGAGGGCAGCCGCGGCAAGGAGGCAGGGGAGGGCGGCCTACCGCTGTGCGGGCCCACG
  GACAAGACCCCACTGCTGAGCGGCAAGGCCGCCAAGGCCCGGGACCGCGAGGTCGACCTCAAGAATGGCC
  ACAACCTCTTCATCTCTGCGGCGGCCGTGCCTCCCGGAAGCCTCCTCAGCGGCCCCGGCCTGGCCCCGGC
  GGCGTCCTCCGCAGGCGGCGCGGCGTCCTCCGCCCAGACGCACCGGTCCTTCCTGGGCCCCTTCCCGCCG
  GGACCGCAGTTCGCGCTCGGCCCCATGTCCCTGCAGGCCAACCTCGGCTCCGTGGCCGGCTCCTCCGTGC
  TGCAGTCGCTGTTCAGCTCTGTGCCGGCCGCCGCAGGCCTGGTGCACGTGTCGTCCGCTGCCACCAGACT
  GACCAACTCGCACGCCATGGGCAGCTTTTCCGGGGTGGCAGGCGGCACAGTTGGAGGTAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218547 representing NM_032482
  Red=Cloning site Green=Tags(s)
  
  MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFES
  MQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETS
  FDLVAQMIDEIKMTDDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYE
  KKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRIN
  SRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESK
  SNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTA
  NPERKPKKNQTALDALHAQTVSQTAASSPQDAYRSPHSPFYQLLPSVQRHSPNPLLVAPTPPALQKLLES
  FKIQYLQFLAYTKTPQYKASLQELLGQEKEKNAQLLGAAQQLLSHCQAQKEEIRRLFQQKLDELGVKALT
  YNDLIQAQKEISAHNQQLREQSEQLEQDNRALRGQSLQLLKARCEELQLDWATLSLEKLLKEKQALKSQI
  SEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDDALSLHLRGKGALGRELEPDASRLHLELDCTKFS
  LPHLSSMSPELSMNGQAAGYELCGVLSRPSSKQNTPQYLASPLDQEVVPCTPSHVGRPRLEKLSGLAAPD
  YTRLSPAKIVLRRHLSQDHTVPGRPAASELHSRAEHTKENGLPYQSPSVPGSMKLSPQDPRPLSPGALQL
  AGEKSSEKGLRERAYGSSGELITSLPISIPLSTVQPNKLPVSIPLASVVLPSRAERARSTPSPVLQPRDP
  SSTLEKQIGANAHGAGSRSLALAPAGFSYAGSVAISGALAGSPASLTPGAEPATLDESSSSGSLFATVGS
  RSSTPQHPLLLAQPRNSLPASPAHQLSSSPRLGGAAQGPLPEASKGDLPSDSGFSDPESEAKRRIVFTIT
  TGAGSAKQSPSSKHSPLTASARGDCVPSHGQDSRRRGRRKRASAGTPSLSAGVSPKRRALPSVAGLFTQP
  SGSPLNLNSMVSNINQPLEITAISSPETSLKSSPVPYQDHDQPPVLKKERPLSQTNGAHYSPLTSDEEPG
  SEDEPSSARIERKIATISLESKSPPKTLENGGGLAGRKPAPAGEPVNSSKWKSTFSPISDIGLAKSADSP
  LQASSALSQNSLFTFRPALEEPSADAKLAAHPRKGFPGSLSGADGLSPGTNPANGCTFGGGLAADLSLHS
  FSDGASLPHKGPEAAGLSSPLSFPSQRGKEGSDANPFLSKRQLDGLAGLKGEGSRGKEAGEGGLPLCGPT
  DKTPLLSGKAAKARDREVDLKNGHNLFISAAAVPPGSLLSGPGLAPAASSAGGAASSAQTHRSFLGPFPP
  GPQFALGPMSLQANLGSVAGSSVLQSLFSSVPAAAGLVHVSSAATRLTNSHAMGSFSGVAGGTVGGN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032482
• ORF Size: 4611 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032482.1, NP_115871.1
• RefSeq Size: 7455 bp
• RefSeq ORF: 4614 bp
• Locus ID: 84444
• UniProt ID: Q8TEK3
• Cytogenetics: 19p13.3
• Domains: AT_hook
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• Gene Summary: The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]